Biochemical characterization of highly organic solvent-tolerant cutinase from Fusarium oxysporum

Structural and functional studies of a Fusarium oxysporum cutinase with polyethylene terephthalate modification potential

“…5A). A crude cutinase preparation from a wild-type F. oxysporum strain CBMAI 1274, exhibited optimal activity at 28 ο C [60], while cut1 from F. solani expressed in P. pastoris showed an optimum temperature of 40 ο C [61]. Fungal cutinases that have been heterologously expressed in different expression systems exhibit optimal catalytic activity at temperatures varying from 25 ο C (a cutinase from G. cingulata expressed in E. coli Origami B [62]) to 50 ο C (a cutinase from H.…”

Characterization of an acidic cold-adapted cutinase from Thielavia terrestris and its application in flavor ester synthesis

“…Cutinases have been purified and characterized from various fungi (Fraga et al, 2012;Koschorreck et al, 2010;Kwon et al, 2009;Roussel et al, 2014;Speranza & Macedo, 2013;Wang et al, 2002), especially phytopathogenic fungi such as Fusarium solani pisi, whose cutinase has been extensively studied with respect to its structure and function. However there are no reports Optimal pH (a), pH stability (b), optimal temperature (c), thermostability (d) and thermal denaturation half lives (e) of TtcutB from T. terrestris.…”

“…Cutinases are mainly found in different species of fungi (Castro-Ochoa et al, 2012;Fraga, Carvalho, & Macedo, 2012;Nyyssölä et al, 2014;Pio & Macedo, 2007;Roussel et al, 2014;Speranza & Macedo, 2013), although several bacterial cutinolytic enzymes have also been reported (Dutta, Krishnamoorthy, & Dasu, 2013;Hegde & Veeranki, 2013;Kitadokoro et al, 2012). A number of cutinases have been purified and characterized from various fungi, including Alternaria brassicicola (Koschorreck, Liu, Kazenwadel, Schmid, & Hauer, 2010), Coprinopsis cinerea (Merz, Schembecker, Riemer, Nimtz, & Zorn, 2009), Fusarium oxysporum (Fraga et al, 2012;Speranza & Macedo, 2013), Fusarium solani (Kwon, Kim, Yang, Song, & Song, 2009), Monilinia fructicola (Wang, Michailides, Hammock, Lee, & Bostock, 2002), Sirococcus conigenus , Trichoderma harzianum (Rubio, Cardoza, Hermosa, Gutierrez, & Monte, 2008) and Trichoderma reesei (Roussel et al, 2014). Most of these are mesophilic fungi; there are no reports on cutinases from thermophilic fungi, except for Humicola insolens (Nielsen, Borch, & Westh, 2007).…”

Lipase catalyzed interesterification of Amazonian patauá oil and palm stearin for preparation of specific-structured oils

“…Palm stearin (melting point 43°C), solid fraction obtained by the fractionation of the palm oil Elaeis guineensis Jacq (Arecaceae), was kindly supplied by Agropalma (Pará, Brazil). Crude lipase from Rhizopus sp was produced in our laboratory (Macedo et al 2004 andMacedo 2013). Commercial, purified and immobilized lipase (Lipozyme TL-IM) was kindly supplied by Novozymes.…”

“…Lipase from Rhizopus sp was produced according to Macedo et al (2004) and Speranza and Macedo (2013) in your laboratory. Briefly, the microorganism was grown in solid medium composed of wheat bran (40 % water w/w) for 72 h at 30°C.…”