Biochemical characterization of progesterone-induced alterations in bovine uterine fluid amino acid and carbohydrate composition during the conceptus elongation window†

Simintiras, Constantine A; Sánchez, José María; McDonald, Michael; Martins, Thiago; Binelli, Mário; Lonergan, P.

doi:10.1093/biolre/ioy234

Cited by 28 publications

(34 citation statements)

References 89 publications

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“…More specifically, only these two metabolites increased on Days 13 and 14 vs. 12 within the normal P4 group. Moreover, fructose and mannitol/sorbitol were elevated by 18.4 and 28.4-fold, respectively, in the ULF of high vs. normal P4 heifers on Day 12 (Simintiras et al, 2019a) -the greatest differences observed throughout the study -suggestive of a key role for these metabolites in sustaining, in addition to initiating, conceptus elongation.…”

Section: Uterine Lumen Fluid Compositonmentioning

confidence: 89%

“…Given that conceptus elongation coincides with a period of significant bovine pregnancy loss, our aim was to achieve a better understanding of the biochemical landscape surrounding the peri-elongation conceptus. Over 5000 metabolites were screened for by highthroughput untargeted ultra-high-performance liquid chromatography tandem mass spectroscopy, with 233 consistently identified, clustering within 8 superpathways: amino acids, carbohydrates (Simintiras et al, 2019a), lipids (Simintiras et al, 2019b), cofactors, vitamins, nucleotides, peptides, energy substrates, and xenobiotics (Simintiras et al, 2019c). A global analysis of this dataset revealed three core 'strategies' likely utilised by the bovine endometrium to facilitate conceptus elongation, discussed below.…”

Section: Uterine Lumen Fluid Compositonmentioning

confidence: 99%

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Aspects of embryo-maternal communication in establishment of pregnancy in cattle

2019

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Establishment of pregnancy in mammals requires reciprocal molecular communication between the conceptus and endometrium that modifies the endometrial transcriptome and uterine luminal milieu to support pregnancy. Due to the small size of the early embryo and elongating conceptus relative to the volume of the uterine lumen, collection of endometrium adjacent to the developing conceptus is difficult following conventional uterine flushing methods in cattle. Use of endometrial explants in culture can overcome this challenge and reveal information about the dialogue between the developing embryo and the uterus. The aim of this short review is to summarize some of our recent findings in relation to embryo maternal interaction during bovine pregnancy establishment and to put them in the wider context of fertility in cattle.

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Section: Uterine Lumen Fluid Compositonmentioning

confidence: 89%

Section: Uterine Lumen Fluid Compositonmentioning

confidence: 99%

Aspects of embryo-maternal communication in establishment of pregnancy in cattle

2019

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“…In vitro studies have shown that the bovine embryo begins to consume substantial arginine at the 8-cell stage [72], and that arginine addition to culture medium improve blastocyst formation and hatching [73]. In vivo, dietary arginine supplementation improves porcine reproductive performance [74], and in the bovine, porcine, and ovine, arginine has been identified as a key player in peri-elongation embryo development [75][76][77][78]. These results, coupled with the increased arginine observed in the isthmus-but not the ampulla-of pregnant vs. cyclic heifers ( Figure 5A), suggest an important role for arginine in early bovine embryo development.…”

Section: Discussionmentioning

confidence: 99%

Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid

Rodríguez‐Alonso

Maillo

Acuña

et al. 2020

IJMS

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Knowledge of how the biochemical composition of the bovine oviduct is altered due to the oviduct anatomy or the presence of an embryo is lacking. Thus, the aim of this study was to assess the effect of (I) oviduct anatomy and (II) embryo presence on oviductal fluid (OF) protein, amino acid, and carbohydrate composition. Cross-bred beef heifers (n = 19) were synchronized and those in standing estrus were randomly allocated to a cyclic (non-bred) or pregnant (artificially inseminated) group. All heifers were slaughtered on Day 3 after estrus. The oviducts ipsilateral to the corpus luteum from each animal were isolated, straightened and cut, separating ampulla and isthmus. Each portion was flushed with 500 µl of PBS enabling recovery of the oocyte/embryo. Recovered unfertilized oocytes (cyclic group) and embryos (8-cell embryos; pregnant group) were located in the isthmus of the oviduct. Samples of flushing medium from the isthmus and ampulla were used for proteomic (n = 2 per group), amino acid (n = 5), and carbohydrate (n = 5) analysis. For proteomic analysis, total protein from cyclic and pregnant samples were labelled with different cyanine fluorescent probes and separated according to the isoelectric point using immobilized pH gradient strips (pH 3-10, 17 cm, Protean ® IEF cell system, Bio Rad). Second dimension was performed in a polyacrylamide gel (12%) in the presence of SDS using a Protean II XL system (Bio Rad). Images were obtained with a Typhoon 9410 scanner and analyzed with Progenesis SameSpots software v 4.0. Amino acid content in the OF was determined by high performance liquid chromatography (HPLC). Glucose, lactate, and pyruvate were quantified using microfluorometric enzyme-linked assays. For the proteomic assessment, the results of the image analysis were compared by ANOVA. For both amino acid and carbohydrate analyses, statistical analysis was carried out by 2-way ANOVA with the Holm-Sidak nonparametric post hoc analysis. On Day 3 post-estrus, OF composition varied based on (I) anatomical region, where isthmic metabolites were present in lower (i.e., lactate, glycine, and alanine) or higher (i.e., arginine) concentrations compared to the ampulla; and (II) embryo presence, which was correlated with greater, arginine, phosphoglycerate kinase 1, serum albumin, α-1-antiproteinase and IGL@ Int.The oviduct is a tubular seromuscular organ connecting the ovary to the uterus providing a physiological environment for gamete interaction, fertilization, and early embryo development. During the first four days of embryo development, which occurs within the oviduct [1], embryonic nutritional requirements are presumed to be fulfilled largely by oviduct fluid (OF)-a complex mixture of proteins, amino acids, carbohydrates, ions, hormones, and extracellular vesicles, amongst other constituents [2]. OF composition is regulated by (I) selective transudate from the vasculature, and (II) the secretory cells within the oviduct epithelial monolayer [3]. Moreover, OF composition is influenced spatially by the anatomical...

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“…Elevated P4 during the preimplantation stage has been shown to positively influence embryo growth in mice (Aisemberg et al, 2013), ruminants (Kleemann et al, 1994;Inskeep, 2004;Clemente et al, 2009;O'Hara et al, 2014a), alpacas (Bravo and Diaz, 2010), and pigs (Ashworth, 1991;Jindal et al, 1997). This is, at least in part, due to P4-stimulated endometrial secretions, collectively termed histotroph, which support conceptus development, implantation, and placentation (Simintiras et al, 2019). Thus, the SPinduced increase in P4 likely benefits embryo development and subsequent survival.…”

Section: Introductionmentioning

confidence: 99%

Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression

Mateo‐Otero

Sánchez

Recuero

et al. 2020

Front. Cell Dev. Biol.

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A growing body of evidence suggests that paternal factors have an impact on offspring development. These studies have been mainly carried out in mice, where seminal plasma (SP) has been shown to regulate endometrial gene expression and impact embryo development and subsequent offspring health. In cattle, infusion of SP into the uterus also induces changes in endometrial gene expression, however, evidence for an effect of SP on early embryo development is lacking. In addition, during natural mating, the bull ejaculates in the vagina; hence, it is not clear whether any SP reaches the uterus in this species. Thus, the aim of the present study was to determine whether SP exposure leads to improved early embryo survival and developmental rates in cattle. To this end, Day 7 in vitro produced blastocysts were transferred to heifers (12-15 per heifer) previously mated to vasectomized bulls (n = 13 heifers) or left unmated (n = 12 heifers; control). At Day 14, heifers were slaughtered, and conceptuses were recovered to assess size, morphology and expression of candidate genes involved in different developmental pathways. Additionally, CL volume at Day 7, and weight and volume of CL at Day 14 were recorded. No effect of SP on CL volume and weight not on conceptus recovery rate was observed. However, filamentous conceptuses recovered from SP-exposed heifers were longer in comparison to the control group and differed in expression of CALM1, CITED1, DLD, HNRNPDL, PTGS2, and TGFB3. In conclusion, data indicate that female exposure to SP during natural mating can affect conceptus development in cattle. This is probably achieved through modulation of the female reproductive environment at the time of mating.

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Biochemical characterization of progesterone-induced alterations in bovine uterine fluid amino acid and carbohydrate composition during the conceptus elongation window†

Cited by 28 publications

References 89 publications

Aspects of embryo-maternal communication in establishment of pregnancy in cattle

Aspects of embryo-maternal communication in establishment of pregnancy in cattle

Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid

Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression

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