Biochemical characterization of recombinant Penaeus vannamei trypsinogen

Identification and in silico structural and functional analysis of a trypsin-like protease from shrimpMacrobrachium carcinus

“…This tetra-Asp sequence together with Asp217 (chymo#) has been involved in an inhibitory function for trypsin-mediated trypsinogen activation (autoactivation), by an electrostatic repulsion between Asp217 (chymo#) and the tetra-aspartate (Nemoda & Sahin-Tóth, 2007). Therefore, the corresponding trypsinogen of the Mc-TryL protease likely has an increased autoactivation rate in comparison with other non-crustacean trypsins in a similar manner as we have recently described for the P. vannamei trypsinogen (Guerrero-Olazarán et al, 2019), although with slight differences due to the lack of Arg or Lys at the end of the propeptide. Since the storage of active trypsin is a risk of tissue damage through hydrolysis of autologous protein, M. carcinus must possess a tightly regulated mechanism for controlling activation until needed for a precise function similar to other crustaceans.…”

“…Since the storage of active trypsin is a risk of tissue damage through hydrolysis of autologous protein, M. carcinus must possess a tightly regulated mechanism for controlling activation until needed for a precise function similar to other crustaceans. The control of trypsin activity in crustacea is a fine-tuning mechanism that involves the continuous regulation of trypsin activity through zymogen storage, secretion and activation like frequent feeder species (Sainz et al, 2004;Guerrero-Olazarán et al, 2019), with a main role of trypsin inhibitors for controlling trypsin activity and/or trypsinogen activation (García-Carreño, Carrillo & Navarrete del Toro, 1999;De Albuquerque-Cavalcanti, García-Carreño & Navarrete del Toro, 2002;Guerrero-Olazarán et al, 2019).…”

“…The conversion of trypsinogen to active trypsin is initiated by the specific cleavage of a small N-terminal peptide by an autoactivation process or in mammals, by the action of enterokinase. In the shrimp Penaeus vannamei, trypsin synthesis from trypsinogen has been proposed from sequence analysis (Klein et al, 1996), the demonstration of trypsinogen storage in the midgut gland has been proved (Sainz et al, 2004), and recently we demonstrated a rapid trypsinogen autoactivation process (Guerrero-Olazarán et al, 2019).…”

Efectos del metabisulfito de sodio sobre la actividad y composición proteásica de los extractos enzimáticos de Pleoticus muelleri

“…Esto indicaría que a pesar de que la actividad de las enzimas alcalinas se vio afectada negativamente por el uso del preservante, las tripsinas se encuentran activas en mayor proporción en la composición enzimática total. Por otro lado, la abrupta reducción de la actividad alcalina en los langostinos tratados con MBS podría deberse a que este compuesto ha provocado la ruptura y/o reordenamiento de enlaces disulfuro en las enzimas provocando su inactivación (Zhang y Sun 2008; Guerrero-Olazarán et al 2019). Esto último concuerda con los resultados obtenidos por Friedman y Gumbmann (1986), quienes detectaron una reducción en la actividad inhibidora de tripsinas de harina de soja en presencia de sulfito de sodio.…”