Biochemical characterization of somatic embryogenesis and genetic transformation studies in Terminalia chebula Retz.: An immensely valuable medicinal tree

Anjaneyulu, Ch.; Giri, Charu Chandra

doi:10.21276/ap.2018.7.1.5

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…SE is the capacity of differentiated cells to acquire embryogenic competence (Robert et al, 2015). The feasibility of plantlet regeneration from single cell origin, faster multiplication rate, the possibility of automating the large-scale production of embryos in bioreactors and eld planting as synthetic seeds, direct development of produced embryos into plantlet for their bipolar nature so bypassing the rooting stage needed in organogenic regeneration, are some of the precious advantages of SE (Guan et al, 2016, Ammirato 1983. Plant regeneration via SE passes four steps: (i) initiation of somatic embryos from the primary explant, (ii) proliferation of embryogenic cultures, (iii) maturation of somatic embryos, and (iv) regeneration of plants from somatic embryos (Santos et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis; A Highly Promising Technique for Conservation of Tecomella Undulata – A Multi-Purpose Tree on the Verge of Extinction

Rastgoo

2021

Preprint

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Over-exploitation of trees by increasing human population has put an enormous pressure especially on those species having slow growth and low reproduction rate. Desert teak (Tecomella undulata (Sm.) Seem) a multipurpose agroforestry tree of arid zone has fallen on the verge of extinction in Iran due to lack of efficient seed reproduction. Inherent low adventitious rooting has caused vegetative mass macro- and micropropagation (organogenesis pathway) of this tree to be unsuccessful. Somatic embryogenesis (SE) pathway by production of bipolar embryo capable of development into a complete plantlet can solve the problem. Hence, a research was carried out to evaluate the feasibility of in vitro regeneration of the tree by SE. Ovary extracted from un-pollinated flower was cultured in the media supplemented with different auxins and cytokinins (CKs). The results showed α-naphthalene acetic acid (NAA) was superior in induction of embryogenic callus (EC). NAA ranging 5.4–21.5 µM could induce the highest ECs which exhibited developing pro-embryogenic masses (PEMs) and globular somatic embryos. Both CKs, thidiazuron (TDZ) and N6-benzyladenine (BA), though induced good callogenesis at low concentrations but the formed calli were non-embryogenic. Proliferation of NAA–induced ECs revealed that hormone-free medium is the best choice. However, the media containing 40.5 and 54 µM NAA alone could also induce somatic embryos along with callus proliferation. Effort for maturation of the obtained globular, hear-shaped and torpedo embryos did not yield satisfactory results. Low BA-contained medium led to secondary SE. More research is expected to be undertaken to optimize SE in desert teak.

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Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis; A Highly Promising Technique for Conservation of Tecomella Undulata – A Multi-Purpose Tree on the Verge of Extinction

Rastgoo

2021

Preprint

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“…Wight and Arn. (Family Combertaceae) is most popular and used frequently in many ayurvedic preparations (Alam and Sharma, 2013;Anjaneyulu and Giri, 2018). Every part of this plant contains medicinal value, consequently, Arjuna hold a remarkable position in both Ayurvedic, Siddha and Unani systems of medicine.…”

Section: Introductionmentioning

confidence: 99%

Original article Contemporary medicinal uses of ethnomedicinally important plant: Arjuna [Terminalia arjuna (Roxb.) Wight and Arn.]

Alam¹,

Kanchan²,

Emmanuel³

2019

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Terminalia arjuna (Roxb.) Wight and Arn. (Arjuna tree) is one of the important medicinal plants which belong to the family Combertaceae. This plant is widely distributed in Asia, especially in India and Bangladesh, and has been used extensively in modern herbal preparations related to heart diseases. In ethnobotanical perspective, this plant has great significance and utilized variously by the indigenous tribe. Through, this article is an attempt, has been made to recapitulate its ethnobotanical importances, current uses in healthcare in treatment of various aliments, and its future prospective. The potential of this plant is also highlighted in the context of genetic engineering and tissue culture approaches through that the production of plant cell products can be improved many folds without any serious loss to the diversity of this imperative medicinal plant.

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“…(Gordan and Antolic, 2001). Traditional plants have been played an imperative role in major healthcare needs (Anjaneyulu and Giri, 2018). Plant based natural product including extracts, essential oils, phytomolecules, etc., attract the pharmaceutical industry (Nooreen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Original article In vitro study of antimicrobial activity of Tinosporacordifolia (Thunb.) Miers plant extracts against selected clinical isolates

Devi¹,

Sharma²,

Navdeep³

et al. 2018

Ann. Phytomed

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The present investigation was aimed to study the antimicrobial potential of Tinospora cordifolia (Thunb.) Miers commonly known as "Giloy" which is used by the herbal practitioners for curing several ailments. The antimicrobial potential was explored by using solvent extracts of T. cordifolia stem aga inst clinical pa thogenic isola tes, i.e., S tap hyloco ccu s a ure us, Ba cillus ce reu s, Pseudomonas aeruginosa, Streptococcus pneumoniae and Yersinia pestis. Extraction was carried out by cold percolation method, using various solvents, viz., chloroform, acetone, methanol and water. Among all, the chloroform and acetone extracts of T. cordifolia showed maximum activity against S. aureus (18.22 mm at 4 mg/ml). The minimum inhibitory concentration (MIC) of solvent extract of T. cordifolia was 6 mg in case of S. aureus. It was found that extremely small quantity of crude extract of T. cordifolia was sufficient to inhibit the growth of pathogenic microbes and it was quite comparable to the positive control used in many cases. These encouraging results can be taken further to purify and characterize the plant extracts and to check their antimicrobial activity against the pathogenic bacteria so as to utilize the pharmacological potential of the plant extract at higher scale after extensive R & D efforts.

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Biochemical characterization of somatic embryogenesis and genetic transformation studies in Terminalia chebula Retz.: An immensely valuable medicinal tree

Cited by 3 publications

References 30 publications

Somatic Embryogenesis; A Highly Promising Technique for Conservation of Tecomella Undulata – A Multi-Purpose Tree on the Verge of Extinction

Somatic Embryogenesis; A Highly Promising Technique for Conservation of Tecomella Undulata – A Multi-Purpose Tree on the Verge of Extinction

Original article Contemporary medicinal uses of ethnomedicinally important plant: Arjuna [Terminalia arjuna (Roxb.) Wight and Arn.]

Original article In vitro study of antimicrobial activity of Tinosporacordifolia (Thunb.) Miers plant extracts against selected clinical isolates

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