2011
DOI: 10.4014/jmb.1106.06046
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Biochemical Characterization of the Exopolysaccharide Purified from Laetiporus sulphureus Mycelia

Abstract: The extracellular polysaccharide (EPS) was isolated from mycelial cultures of Laetiporus sulphureus var. miniatus and purified by DEAE cellulose and Sephadex G-50 column chromatography. The purified EPS (EPS-2-1) was composed of only glucose units and its molecular mass was 6.95 kDa. The chemical structure of EPS-2-1 consisted of a main chain containing (1→4)-Glcp units with branches at the C-6 position of the chain carrying-Glcp-(1→4)-linked residues. The effect of purified EPS on immunomodulatory genes and p… Show more

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Cited by 10 publications
(6 citation statements)
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“…While there are many protocols in the literature for isolation of capsular polysaccharides or for the extraction of total bacterial EPS, only a few have attempted to fractionate exopolysaccharides by size [16] and none have been specifically tailored for the isolation of high molecular weight backbone exopolysaccharides from biofilms. Bulk EPS extraction requires methods that physically break up the biofilm matrix such as ultrasonication or EDTA, which promotes EPS separation by chelating cations that are thought to crosslink polysaccharide chains within the EPS.…”
Section: Introductionmentioning
confidence: 99%
“…While there are many protocols in the literature for isolation of capsular polysaccharides or for the extraction of total bacterial EPS, only a few have attempted to fractionate exopolysaccharides by size [16] and none have been specifically tailored for the isolation of high molecular weight backbone exopolysaccharides from biofilms. Bulk EPS extraction requires methods that physically break up the biofilm matrix such as ultrasonication or EDTA, which promotes EPS separation by chelating cations that are thought to crosslink polysaccharide chains within the EPS.…”
Section: Introductionmentioning
confidence: 99%
“…The most important bioactive compounds of this mushroom are lectins Tateno et al, [9][10][11] polysaccharides [12][13][14][15][16][17][18], phenols Olennikov et al, [1,19,20]m terpenoids León et al, [21][22][23], enzymes [24,25], polyene pigments [26], and polyunsaturated fatty acids [27]. The chemical formulas of some of the major bioactive substances L. sulphureus are shown in fi gure 2.…”
Section: Bioactive Substancesmentioning
confidence: 99%
“…Despite the importance of exopolysaccharide in bacterial cariogenicity, how the vicX gene modulates the biofilm exopolysaccharide characteristics has not yet been elucidated well. Although there are many protocols for the extraction of total bacterial polysaccharides in the planktonic growth, only a few methods have attempted to fractionate and analyze the backbone glucans from biofilms (Seo et al, 2011 ; Bales et al, 2013 ).…”
Section: Introductionmentioning
confidence: 99%