Biochemical Characterization of the Phagocytic Giant Cell Receptor forDictyostelium discoideumAmoebae: Identification by Cell Blotting

Lewis, Keith E.; Jamieson, J.C.

doi:10.1006/bbrc.1996.5990

Cited by 2 publications

(4 citation statements)

References 16 publications

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“…While their function in phagocytosis remain questionable, glycoconjugates containing β ‐ D ‐galactose increase in expression/presence in phagocytic ZGCs (O’Day et al , 1987). A novel approach using cell blotting identified two high‐mannose glycoprotein receptors at 40 kDa and 130 kDa that mediated the uptake of amoebae by ZGCs (Lewis & Jamieson, 1997). The addition of tunicamycin, an N ‐linked glycoslation inhibitor, and treatments with mevinolin or mevastatin, both inhibitors of effective mannose glycoprotein formation, were found to inhibit sexual phagocytosis (Lewis & Jamieson, 1997).…”

Section: Heterothallic Sexual Development Of Dictyostelium Discoimentioning

confidence: 99%

“…A central issue during sexual development is the issue of self/non-self recognition. While important in gamete recognition, the issue is especially relevant as the recognition systems developed by the cannibalistic zygote cell come into play (Lewis & Jamieson, 1997). Phagocytosis in sexual development is selective.…”

Section: (D) Self Versus Non-self Recognitionmentioning

confidence: 99%

“…A novel approach using cell blotting identified two high-mannose glycoprotein receptors at 40 kDa and 130 kDa that mediated the uptake of amoebae by ZGCs (Lewis & Jamieson, 1997). The addition of tunicamycin, an N -linked glycoslation inhibitor, and treatments with mevinolin or mevastatin, both inhibitors of effective mannose glycoprotein formation, were found to inhibit sexual phagocytosis (Lewis & Jamieson, 1997). Whether these receptors are directly involved in the phagocytic process or serve to anchor the target cell at the cell surface remains to be elucidated.…”

Section: (D) Self Versus Non-self Recognitionmentioning

confidence: 99%

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Signalling and sex in the social amoebozoans

O’Day

Keszei

2011

Biological Reviews

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The social amoebozoans have a life tricycle consisting of asexual multicellular development leading to fruiting bodies, sexual multicellular development resulting in macrocysts, and unicellular development generating microcysts. This review covers the events of sexual development in the best-studied heterothallic (Dictyostelium discoideum) and homothallic (D. mucoroides) mating systems. Sexual development begins with pheromonal interactions that produce fusion-competent cells (gametes) which undergo cell and pronuclear fusion. Calcium- and calmodulin-mediated signalling mediates these early events. As they initiate chemotactic signalling, each zygote increases in size becoming a zygote giant cell. Using cyclic AMP (cAMP), the zygote chemotactically lures in amoebae and engulfs them in an act of cannibalistic phagocytosis. Chemotaxis proceeds more quickly than endocytosis because the breakdown products of cAMP (5-AMP, adenosine) bind to a presumptive adenosine receptor to inhibit sexual phagocytosis. This slowing of phagocytosis allows amoebae to accumulate around the zygote to form a precyst aggregate. Zygote giant cells also produce several other signalling molecules that feed back to regulate early events. The amoebae surrounding the zygote seal their fate as zygotic foodstuff by secreting a primary cellulose wall, the extracellular sheath, around the zygote and aggregated amoebae, which prevents their escape. Phagocytosis within this precyst continues until all peripheral amoebae are internalized as endocytes and the final macrocyst wall is formed. Endocyte digestion results in a mature macrocyst with a uniform cytoplasm containing a diploid nucleus. After detailing the morphological events of heterothallic and homothallic mating, we review the various intercellular signalling events and other mechanisms involved in each stage. This complete and comprehensive review sets the stage for future research on the unique events that characterize sex in the social amoebozoans.

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Section: Heterothallic Sexual Development Of Dictyostelium Discoimentioning

confidence: 99%

Section: (D) Self Versus Non-self Recognitionmentioning

confidence: 99%

Section: (D) Self Versus Non-self Recognitionmentioning

confidence: 99%

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Signalling and sex in the social amoebozoans

O’Day

Keszei

2011

Biological Reviews

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“…Within the Dictyostelium clade, Mizutani, Hagiwara, and Yanagisawa [55][56][57] have described several secreted factors from Polysphondylium pallidum that seem to be small proteins that inhibit the growth of other isolates of P. pallidum or the cells of The sexual development of D. discoideum and related species is not as well studied as asexual development, but is significant for our purposes [69,70]. During sexual development, favored by the presence of two mating types (A and a) and moist conditions, two haploid cells fuse to form what is known as a zygote [71][72][73][74]. This large and motile cell then does the extraordinary.…”

Section: Inhibitory Moleculesmentioning

confidence: 99%

The Cold War of the Social Amoebae

Shaulsky

Kessin

2007

Current Biology

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When confronted with starvation, the amoebae of Dictyostelium discoideum initiate a developmental process that begins with cell aggregation and ends with a ball of spores supported on a stalk. Spores live and stalk cells die. Because the multicellular organism is produced by cell aggregation and not by growth and division of a single cell, genetically diverse amoebae may enter an aggregate and, if one lineage has a capacity to avoid the stalk cell fate, it may have a selective advantage. Such cheater mutants have been found among wild isolates and created in laboratory strains. The mutants raise a number of questions--how did such a cooperative system evolve in the face of cheating? What is the basis of self recognition? What genes are involved? How is cheating constrained? This review summarizes the results of studies on the social behavior of Dictyostelium and its relatives, including the familiar asexual developmental cycle and the lesser known, but puzzling, sexual cycle.

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Biochemical Characterization of the Phagocytic Giant Cell Receptor forDictyostelium discoideumAmoebae: Identification by Cell Blotting

Cited by 2 publications

References 16 publications

Signalling and sex in the social amoebozoans

Signalling and sex in the social amoebozoans

The Cold War of the Social Amoebae

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