Biochemical composition of microfilarial and adult stages ofSetaria cervi

Abstract

The microfilariae and adults of Setaria cervi, a filarial parasite of Indian water-buffalo (Bubalus bubalis Linn.) and of other ruminants were analysed for various biochemical constituents. Both stages of the parasite were rich in protein, carbohydrate, and lipids while nucleic acids were detectable in only small amounts. Microfilariae and adults contained 10 and 25% dry matter, 57.4 and 63.0% protein, 8.9 and 14.8% carbohydrate, and 12.8 and 9.2% lipid, respectively. Reducing sugars accounted for 52 and 67% respectively of the total carbohydrate in these two stages. Glycogen accounted for 95% of total carbohydrate in the cold PCA soluble fraction of adult worms while its concentration in microfilariae was very low. Glucose was the main constituent of reducing sugars while concentrations of fructose were very low. Microfilariae contained more lipids, nucleic acids, mucopolysaccharides, and total phosphorus than adults. The phospholipid percentage was higher in microfilariae than in adults. The amino acid pattern of both stages was very similar.

Two decades of antifilarial drug discovery: a review

“…Dopamine-b-hydroxylase has also been observed in L. carinii and D. viteae. [212][213][214] Octapamine, an amine identied in B. pahangi, was found to regulate various important processes in nematodes. This biogenic amine was also the only single amine without an important role in mammalian biological processes.…”

Biochemical composition and metabolic pathways of filarial worms Setaria cervi: search for new antifilarial agents

“…Adults and mf of S . cervi have been analysed for various biochemical constituents (Rathaur et al , 1980; Malhotra et al , 1986; Kumar, 1987a, b). Both stages of the parasite are rich in protein, carbohydrate and lipids, while nucleic acids are detectable in only small amounts.…”

“…All the major classes of lipids have been demonstrated in S . cervi adults (Ansari et al , 1973) as well as mf (Rathaur et al , 1980). The phospholipid percentage is higher in mf than in adults.…”

“…The phospholipid percentage is higher in mf than in adults. The nucleic acid content of adults and mf revealed 0.3 and 1.44% of DNA and 0.6 and 1.29% RNA of the dry weight respectively (Rathaur et al , 1980). The lipid composition of S .…”

Immunohistochemical Study on lipid Catabolism in Adult Onchocerca Volvulus

“…It is known that the worms readily incorporate labelled lipid precursors into their neutral and phospholipid fractions in their lipid bio-synthesis [14,15,16]. The entire major classes of neutral lipids namely, triacylglycerols, diacylglycerols sterols, sterol esters, hydrocarbons and traces of free fatty acids have been verified in Dirofilaria immitis [13,16,17,18] while triacylglycerols and sterols have been demonstrated in adult Setaria cervi [19,20].The adult Brugia pahangi has been reported to have triacylglycerols, sterols, as well as free fatty acids and hydrocarbon fractions in their tissues [18]. All the major phospholipid classes have also been identified in adult filarial worms of D. immitis, B. pahangi, Litomosoides carinii and S. cervi [13,19,21,22].…”