Biochemical composition of the fluid of ovarian cysts and pre-ovulatory follicles compared to the serum in sows

Stankiewicz, Tomasz

doi:10.15653/tpg-140836

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…We studied protein from follicular fluid of the organ of pig that do not use such as ovaries. The ovaries of animal such as porcine have secreted sex hormones such as estrogen, progesterone for developing of the ovulation (Stankiewicz, T., 2015). The ovaries fall out of the ovary when ovulation.…”

Section: Introductionmentioning

confidence: 99%

Technology Management in Genetics

Tungkasen

2019

JISTM

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Technology management in genetics course utilizes laboratory techniques and instrumentation to study genetics involves molecular genetics such as DNA, RNA or protein. Specifically, the variety of technologies methods to suit different learning styles including media or video bring benefits and the knowledge base is important to develop the learner. This research has been shown the technology of genetics to teach and learning for science by using videos, demonstrations and the journal of protein analysis which is the SDS-PAGE technique used. The result showed the technologies management method is good to be applied for students in the course of genetics. After the student watched media, video, and demonstrations the SDS-PAGE technique they can use the SDS-PAGE to analysis the proteins. The result has shown that the protein patterns from different follicle sizes. The porcine follicles ranged from 1-2 and 3-4 millimeters (mm.) follicles. As a result, showed protein patterns composed of 18 to ≥ 220 kDa which were found in follicle samples from all sizes. Moreover, the notification of the porcine follicles in diameter of 3-4 mm. has been found the protein bands at 100 and 120 kDa, in accordance to a study by Miyamoto et al., 1985 which report 100 kDa was Inhibin. The study of Nagyova in 2004 found that the protein band of 120 kDa was inter alpha trypsin inhibitor family. The information of proteins can collect into the computer system which is able to use to develop technology genetics applications for protein studies. This research shows that technologies’ management will support and have benefits for the learners. They can improve their learning and scientific skills. In the other hands, the development of new instruction media and teaching techniques are important and necessary for continuing works through the scientific process systematically.

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Section: Introductionmentioning

confidence: 99%

Technology Management in Genetics

Tungkasen

2019

JISTM

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Alternariol disturbs oocyte maturation and preimplantation development

Schoevers

Santos

2019

Mycotoxin Res

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Hypothyroidism affects lipid and glycogen content and peroxisome proliferator-activated receptor δ expression in the ovary of the rabbit

Rodríguez-Castelán¹,

Méndez-Tepepa²,

Rodríguez-Antolín³

et al. 2018

Reprod. Fertil. Dev.

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Dyslipidaemia and hyperglycaemia are associated with ovarian failure and both have been related to hypothyroidism. Hypothyroidism promotes anovulation and ovarian cysts in women and reduces the size of follicles and the expression of aromatase in the ovary of rabbits. Considering that ovarian steroidogenesis and ovulation depend on lipid metabolism and signalling, the aim of the present study was to analyse the effect of hypothyroidism on the lipid content and expression of peroxisome proliferator-activated receptor (PPAR) δ in the ovary. Ovaries from female rabbits belonging to the control (n=7) and hypothyroid (n=7) groups were processed to measure total cholesterol (TC), triacylglycerol (TAG) and glycogen content, as well as to determine the presence of granules containing oxidized lipids (oxysterols and lipofuscin) and the relative expression of perilipin A (PLIN-A) and PPARδ. Hypothyroidism increased TC and glycogen content, but reduced TAG content in the ovary. This was accompanied by a reduction in the expression of PLIN-A in total and cytosolic extracts, changes in the presence of granules containing oxidative lipids and low PPARδ expression. The results of the present study suggest that hypothyroidism modifies the content and signalling of lipids in the ovary, possibly affecting follicle maturation. These results could improve our understanding of the association between hypothyroidism and infertility in females.

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Biochemical composition of the fluid of ovarian cysts and pre-ovulatory follicles compared to the serum in sows

Abstract: The differences in the biochemical composition of the fluid in follicular cysts and pre-ovulatory follicles point to the variable intensification of the course of metabolic processes in pathological and physiological ovarian structures.

Cited by 3 publications

References 27 publications

Technology Management in Genetics

Technology Management in Genetics

Alternariol disturbs oocyte maturation and preimplantation development

Hypothyroidism affects lipid and glycogen content and peroxisome proliferator-activated receptor δ expression in the ovary of the rabbit

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