Biochemical, Cytochemical and Electron Microscopic Observations on the Enhancement of the Pancreatic Acinar Cell Secretory Activity in the Rat during Early Postnatal Growth

Sesso, Antônio; Carneiro, João José; Cruz, Amanda Rodrigues; Leite, J. B. de Arruda

doi:10.1679/aohc1950.35.343

Cited by 12 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sessoet al [1973], com paring the newly born rat with the adult one, observed in the first instance a very small amount of RER which already develops in the first 48 hours. On the other hand, the level of cytoplasmic RNA is low, as reported by Sesso et al [1973], during the first 2 postnatal weeks, reaching levels from 20 to 40 days for adult animal.…”

Section: Discussionmentioning

confidence: 58%

“…Sesso et al [1973] report a big quan tity of voluminous zymogen granules in the first postnatal days (also a higher number of condensing vacuoles), more numerous and of lesser bulk in the more mature animal, besides a lesser extrusion in the first 15 postnatal days, as well as an increase in synthesis followed by extrusion. Nevertheless, they affirm that there is higher synthesis than extrusion at the 30th-to 40th-day stage, a fact which might corres pond to an increase in the volume occupied in the cell by the zymogen granules; notwith standing, on morphometry there would be a decrease.…”

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

Ultrastructural morphometric study on developing acinar cells of the rat pancreas and parotid gland

Taga

et al. 1978

Cells Tissues Organs

View full text Add to dashboard Cite

The fraction of cellular volume occupied by the cytoplasm has been assessed by point-counting volumetry in 0.5-μm araldite sections. Measurement of the transection radius of the nuclei of acinar cells allowed the assessment of the mean nuclear volume according to the method of Bach. With these data, the cytoplasm volume was calculated in cubic micrometers. Ultrastructural morphometric data were obtained by means of superimposing on the electron photomicrographies (× 21,000 or × 28,500) a test system of 84 evenly spaced segments according to Weibel et al. The analysis of the values obtained for the pancreas and parotid gland allowed for the supposition that the process of maturation in the rat, either for the pancreas or the parotid gland, occurs simultaneously with the mitotic process, this last being held as the predominating one in the first 3–4 postnatal weeks. The cytodifferentiation process becomes more marked in the last phases studied and on the 40th day it already shows parameters held as similar to those of the adult animal.

show abstract

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 95%

Ultrastructural morphometric study on developing acinar cells of the rat pancreas and parotid gland

Taga

et al. 1978

Cells Tissues Organs

View full text Add to dashboard Cite

show abstract

“…Nonetheless, our results show that incorporation of labeled leucine into protein proceeds at a slower rate in the suckling than in the adult rat. Utilizing different experi mental conditions, slower rates of tritiated leucine incorporation into protein in suckling [19] and late fetal [20] pancreas compared to adult have been reported previously. It is not clear whether the decreased rates of incorpo ration are due to changes in the precursor pool of amino acids or to a true difference in the rate of protein synthesis.…”

Section: Discussionmentioning

confidence: 78%

Isolated Pancreatic Acini from Suckling and Weanling Rats: Changes in Amino Acid Incorporation and Carbachol-Stimulated Amylase Secretion with Age

Pollack¹,

Verbridge²,

Thornburg³

et al. 1986

Neonatology

View full text Add to dashboard Cite

To characterize the changes in pancreatic function during postnatal development, isolated pancreatic acini were prepared from rats aged 8–9, 12–14 and 20 days and from adult rats. Isolated acini maintained a normal microscopic appearance and viability as judged by exclusion of trypan blue and linear incorporation of tritiated leucine into total protein. The rate of incorporation in 8-day-old acini was 20% of that observed in adult rats. Significant dose-dependent increases in amylase release in response to carbachol were observed in all age groups; stimulated amylase secretion was significantly less in the 8- to 9- and 12- to 14-day-old animals than in the 20-day-old and adult rats. These data indicate that viable isolated pancreatic acini can be prepared from suckling rats and that these acini exhibit an altered in vitro responsiveness to carbachol. This preparation should therefore be a useful model for in vitro studies of the development of pancreatic function.

show abstract

“…SESSO et al (1973b) and ERMAK and ROTHMAN (1980) observed in newborn rat that the pancreatic acinar cell secretory granules were much larger than those from older rats, and REDMAN and SREEBNY (1971) described an opposite secretory granule diameter variation in acinar cells of the developing parotid gland of the rat. The mechanisms underlying secretory granule diameter increase and decrease in developing terminal tubule and acinar cells are as yet undetermined.…”

Section: Discussionmentioning

confidence: 99%

Morphometric Studies on Terminal Tubule and Acinar Cells in Developing Submandibular Gland of the Rat.

Taga

Alvares

Sesso

1993

Archives of Histology and Cytology

Self Cite

View full text Add to dashboard Cite

Biochemical, Cytochemical and Electron Microscopic Observations on the Enhancement of the Pancreatic Acinar Cell Secretory Activity in the Rat during Early Postnatal Growth

Cited by 12 publications

References 25 publications

Ultrastructural morphometric study on developing acinar cells of the rat pancreas and parotid gland

Ultrastructural morphometric study on developing acinar cells of the rat pancreas and parotid gland

Isolated Pancreatic Acini from Suckling and Weanling Rats: Changes in Amino Acid Incorporation and Carbachol-Stimulated Amylase Secretion with Age

Morphometric Studies on Terminal Tubule and Acinar Cells in Developing Submandibular Gland of the Rat.

Contact Info

Product

Resources

About