Biochemical diversity of global tea [Camellia sinensis (L.) O. Kuntze] germplasm and its exploitation: a review

Kottawa-Arachchi, J. Dananjaya; Gunasekare, M. T. K.; Ranatunga, M. A. B.

doi:10.1007/s10722-018-0698-2

Cited by 34 publications

(27 citation statements)

References 108 publications

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“…2a). The wide range of growing areas, long history of cultivation, self-incompatibility and allogamy of tea plants make these cultivars highly heterogeneous, resulting in high diversity in both their genetics and morphology 145,146 . As the key component of crop genetic improvement, the available germplasms and the genetic diversity within a gene pool determine the success of breeding programs.…”

Section: Genetic Diversity and Population Structure Of Tea Plantsmentioning

confidence: 99%

Tea plant genomics: achievements, challenges and perspectives

et al. 2020

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Tea is among the world’s most widely consumed non-alcoholic beverages and possesses enormous economic, health, and cultural values. It is produced from the cured leaves of tea plants, which are important evergreen crops globally cultivated in over 50 countries. Along with recent innovations and advances in biotechnologies, great progress in tea plant genomics and genetics has been achieved, which has facilitated our understanding of the molecular mechanisms of tea quality and the evolution of the tea plant genome. In this review, we briefly summarize the achievements of the past two decades, which primarily include diverse genome and transcriptome sequencing projects, gene discovery and regulation studies, investigation of the epigenetics and noncoding RNAs, origin and domestication, phylogenetics and germplasm utilization of tea plant as well as newly developed tools/platforms. We also present perspectives and possible challenges for future functional genomic studies that will contribute to the acceleration of breeding programs in tea plants.

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Section: Genetic Diversity and Population Structure Of Tea Plantsmentioning

confidence: 99%

Tea plant genomics: achievements, challenges and perspectives

et al. 2020

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“…The main biological source of the (+)-catechin is green tea [Camelia sinensis (L.) O. Kuntze]. Therefore, green tea is exploited to produce the marketed forms of (+)-catechin, i.e., tablets and dietary supplements [13]. Moreover, reports state that the absorption rate of catechin is very low, which means that the intake of (+)-catechin should be in a more substantial amount to reach an efficient concentration [14].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Flux Analysis of Catechin Biosynthesis Pathways Using Nanosensor

et al. 2020

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(+)-Catechin is an important antioxidant of green tea (Camelia sinensis (L.) O. Kuntze). Catechin is known for its positive role in anticancerous activity, extracellular matrix degradation, cell death regulation, diabetes, and other related disorders. As a result of enormous interest in and great demand for catechin, its biosynthesis using metabolic engineering has become the subject of concentrated research with the aim of enhancing (+)-catechin production. Metabolic flux is an essential concept in the practice of metabolic engineering as it helps in the identification of the regulatory element of a biosynthetic pathway. In the present study, an attempt was made to analyze the metabolic flux of the (+)-catechin biosynthesis pathway in order to decipher the regulatory element of this pathway. Firstly, a genetically encoded fluorescence resonance energy transfer (FRET)-based nanosensor (FLIP-Cat, fluorescence indicator protein for (+)-catechin) was developed for real-time monitoring of (+)-catechin flux. In vitro characterization of the purified protein of the nanosensor showed that the nanosensor was pH stable and (+)-catechin specific. Its calculated Kd was 139 µM. The nanosensor also performed real-time monitoring of (+)-catechin in bacterial cells. In the second step of this study, an entire (+)-catechin biosynthesis pathway was constructed and expressed in E. coli in two sets of plasmid constructs: pET26b-PT7-rbs-PAL-PT7-rbs-4CL-PT7-rbs-CHS-PT7-rbs-CHI and pET26b-T7-rbs-F3H-PT7-rbs- DFR-PT7-rbs-LCR. The E. coli harboring the FLIP-Cat was transformed with these plasmid constructs. The metabolic flux analysis of (+)-catechin was carried out using the FLIP-Cat. The FLIP-Cat successfully monitored the flux of catechin after adding tyrosine, 4-coumaric acid, 4-coumaroyl CoA, naringenin chalcone, naringenin, dihydroquercetin, and leucocyanidin, individually, with the bacterial cells expressing the nanosensor as well as the genes of the (+)-catechin biosynthesis pathway. Dihydroflavonol reductase (DFR) was identified as the main regulatory element of the (+)-catechin biosynthesis pathway. Information about this regulatory element of the (+)-catechin biosynthesis pathway can be used for manipulating the (+)-catechin biosynthesis pathway using a metabolic engineering approach to enhance production of (+)-catechin.

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“…1 According to historical records, tea plants are originated from southwest China, Sichuan, Yunnan, and Guizhou provinces. [2][3][4] Many ancient tea plants are distributed in these provenances of tea plants, and they are important genetic resources for breeding of new improved tea cultivars. Among them, Laochuancha (Camellia sinensis (L.) O. Kuntze.…”

Section: Introductionmentioning

confidence: 99%