Biochemical Effect of Carbaryl on Oxidative stress, Antioxidant enzymes and Osmolytes of Cyanobacterium Calothrix brevissima

Habib, Khalid; Kumar, Satyendra; Manikar, Ningthoujam; Zutshi, Sunaina; Fatma, Tasneem

doi:10.1007/s00128-011-0410-0

Cited by 21 publications

(8 citation statements)

References 28 publications

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“…Although carbaryl and other insecticides can cause oxidative stress and cell lysis in cyanobacteria, the potential effect of carbaryl on the cyanobacteria itself can be excluded. Indeed, the concentrations needed to elicit such a response are a factor 1000 higher than the concentrations used in the present study . Alternatively, the targeted mode of action of carbaryl is the inhibition of the enzyme acetylcholinesterase.…”

Section: Discussionmentioning

confidence: 72%

Combined exposure to cyanobacteria and carbaryl results in antagonistic effects on the reproduction of daphnia pulex

Asselman

Meys

Waegeman

et al. 2013

Environmental Toxicology and Chemistry

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In aquatic ecosystems, Daphnia are exposed to a wide variety of natural and chemical stressors that can cause interactive effects resulting in an increased impact on aquatic ecosystems. The authors therefore investigated the interactive effects of harmful cyanobacteria (cyanoHABs) with carbaryl in Daphnia pulex, because cyanobacteria have become an important concern for aquatic ecosystems. Daphnia were exposed for 21 d to 4 selected cyanobacteria (Aphanizomenon sp., Cylindrospermopsis raciborskii, Microcystis aeruginosa, and Oscillatoria sp), carbaryl, and all binary combinations of carbaryl and each individual cyanobacterium. Results were analyzed with both the independent action and the concentration addition model. The estimated median effect concentration (EC50) for carbaryl was comparable between the experiments, ranging from 2.28 µg/L to 5.94 µg/L. The EC50 for cyanobacteria ranged from 13.45% of the total diet ratio for Microcystis to 66.69% of the diet ratio for Oscillatoria. In combination with carbaryl, the response of Daphnia to 3 of the 4 cyanobacteria demonstrated antagonistic deviation patterns (p< 0.05). Exposure to combinations of carbaryl and Cylindrospermopsis did not result in statistically significant deviations from both reference models. The results may have important implications for pesticide risk assessment, underlining the impact of interactive effects on aquatic organisms. Based on these results, the authors suggest that both the independent action and the concentration addition model can serve as a protective approach in risk assessment of carbaryl in the presence of cyanobacterial blooms.

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Section: Discussionmentioning

confidence: 72%

Combined exposure to cyanobacteria and carbaryl results in antagonistic effects on the reproduction of daphnia pulex

Asselman

Meys

Waegeman

et al. 2013

Environmental Toxicology and Chemistry

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“…CSCs have been described as a subset of cells within a heterogeneous tumor that share a number of features with normal stem cells. CSCs are characterized by self-renewal, the expression of specific surface markers, and aldehyde dehydrogenase (ALDH) activity 8,9 . CSCs are also involved in tumor initiation, metastasis, and chemoresistance 10 .…”

Section: Introductionmentioning

confidence: 99%

High Skp2 expression is associated with a mesenchymal phenotype and increased tumorigenic potential of prostate cancer cells

Šimečková

Kahounová

Fedr

et al. 2019

Sci Rep

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Skp2 is a crucial component of SCF Skp2 E3 ubiquitin ligase and is often overexpressed in various types of cancer, including prostate cancer (PCa). The epithelial-to-mesenchymal transition (EMT) is involved in PCa progression. The acquisition of a mesenchymal phenotype that results in a cancer stem cell (CSC) phenotype in PCa was described. Therefore, we aimed to investigate the expression and localization of Skp2 in clinical samples from patients with PCa, the association of Skp2 with EMT status, and the role of Skp2 in prostate CSC. We found that nuclear expression of Skp2 was increased in patients with PCa compared to those with benign hyperplasia, and correlated with high Gleason score in PCa patients. Increased Skp2 expression was observed in PCa cell lines with mesenchymal and CSC-like phenotype compared to their epithelial counterparts. Conversely, the CSC-like phenotype was diminished in cells in which SKP2 expression was silenced. Furthermore, we observed that Skp2 downregulation led to the decrease in subpopulation of CD44 + CD24 − cancer stem-like cells. Finally, we showed that high expression levels of both CD24 and CD44 were associated with favorable recurrence-free survival for PCa patients. This study uncovered the Skp2-mediated CSC-like phenotype with oncogenic functions in PCa.

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“…Carbaryl could also be generating an oxidative stress situation and inducing antioxidant responses in PC12 cells. Pesticideinduced oxidative stress is the ultimate manifestation of a multistep pathway, resulting in an imbalance between prooxidant and antioxidant defense mechanisms (Banerjee, Seth, & Ahmed, 2001), and affects normal cellular redox status (Habib, Kumar, Manikar, Zutshi, & Fatma, 2011). Carbaryl treatment resulted in a significant increase in the levels of MDA, SOD inhibition rate, and RFI, this suggested induction of oxidative stress to PC12 cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of carbaryl on some biochemical changes in PC12 cells: the protective effect of soy isoflavone genistein, and daidzein, and their mixed solution

Gao

Wang

et al. 2016

CyTA - Journal of Food

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(2016) Effect of carbaryl on some biochemical changes in PC12 cells: the protective effect of soy isoflavone genistein, and daidzein, and their mixed solution, CyTA -Journal of Food, 14:4, 587-593, DOI: 10.1080/19476337.2016 To link to this article: https://doi.org/10. 1080/19476337.2016 We planned to investigate the protective effect of soy isoflavone (SIF) genistein (GEN), and daidzein (DAI), and their mixed solution against carbaryl-induced toxicity in PC12 cells. PC12 cells were exposed to carbaryl, pretreated, co-treatment, or posttreatment with three different SIF solutions for indicated times. Exposure to SIF resulted a better survival and cell morphology which were subjected to carbaryl toxicity. Treatment with SIF was also found to reduce malondialdehyde content, superoxide dismutase inhibitory rate, reactive oxygen species generation, acetylcholine levels in medium, and maintain the integrity of mitochondrial membrane potential. It was also found to increase GSH level. In conclusion, SIF can effectively mitigate adverse effects induced by carbaryl. GEN and DAI have a combined effect that is greater than the effect obtained with each isoflavone alone, although they have different effects against carbaryl-induced damage. SIF enhances metabolism of choline and plays a role as both antioxidant and neuroprotectant.Efecto del carbaril en algunos cambios bioquímicos en células PC12: El efecto protector de las isoflavonas genisteína y daidzeína de la soja, además de su solución mixta RESUMEN Nuestro plan fue investigar el efecto protector de las isoflavonas de la soja (SIF), la genisteína (GEN) y la daidzeína (DAI), además de su solución mixta contra la toxicidad inducida por carbaril en células PC12. Las células PC12 estuvieron expuestas a carbaril, tratadas previamente, tratamiento conjunto o tratadas posteriormente con tres soluciones diferentes de SIF durante los tiempos indicados. La exposición a SIF resultó en una mayor supervivencia y morfología celular, las cuales estuvieron sujetas a la toxicidad del carbaril. También se encontró que el tratamiento con SIF redujo el contenido de MDA, el índice de inhibición de SOD, la generación de ROS, los niveles de acetilcolina a intermedio, además de mantener la integridad del potencial de membrana mitocondrial. También se encontró que hizo aumentar el nivel de GSH. En conclusión, SIF puede mitigar de forma eficiente los efectos adversos inducidos por el carbaril. GEN y DAI tienen un efecto combinado que es mayor que el efecto obtenido con cada isoflavona por separado, aunque tienen efectos distintos contra el daño inducido por carbaril. SIF mejora el metabolismo de la colina, además de jugar un papel importante como antioxidante y neuroprotector.

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Biochemical Effect of Carbaryl on Oxidative stress, Antioxidant enzymes and Osmolytes of Cyanobacterium Calothrix brevissima

Cited by 21 publications

References 28 publications

Combined exposure to cyanobacteria and carbaryl results in antagonistic effects on the reproduction of daphnia pulex

Combined exposure to cyanobacteria and carbaryl results in antagonistic effects on the reproduction of daphnia pulex

High Skp2 expression is associated with a mesenchymal phenotype and increased tumorigenic potential of prostate cancer cells

Effect of carbaryl on some biochemical changes in PC12 cells: the protective effect of soy isoflavone genistein, and daidzein, and their mixed solution

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