Biochemical Effects of Aqueous Extract of Persea americana (Mill) on the Myocardium of Left Ventricle of High Salt–Fed Adult Wistar Rats

Abstract

Background.The cardioprotective effects of Persea americana extract was investigated on biochemical activities of high salt–fed adult Wistar rats in this study.Method.Forty healthy Wistar rats of both sexes weighing 120 to 150 g were randomly assigned into 8 groups of 5 rats each (groups A, B, C, D, E, F, G, and H). Rats in groups A, F, G, and H were fed with standard laboratory pellets, while groups B, C, D, and E were fed on the high-salt diet for 4 weeks. Concomitantly, daily administration of 50, 100, and 150 mg/kg of the P americana extract were given orally to groups C and F, D and G, and E and H, respectively, while rats in groups A and B were administered distilled water. Blood samples were taken by cardiac puncture; concentration of sodium ion, potassium ion, nitric oxide, and activity of lactate dehydrogenase were determined. One-way analysis of variance was used to analyze data, followed by Student-Newman-Keuls (SNK) test for multiple comparison.Results.Results revealed that concentration of potassium ion and nitric oxide was significantly lower (P < .05) in high salt–fed groups. Sodium ion concentration and activity of lactate dehydrogenase were higher in high salt–fed group while P americana prevented biochemical perturbations in other experimental groups.Conclusion.In conclusion, high salt–diet induced biochemical alterations which were significantly protected by oral administration of P americana extract.

Reversal of Hypertension and Amelioration of Oxidative Stress by Persea americana and Allium sativum in Experimentally-Induced Hypertensive Wistar Rats

“…The antihypertensive mechanism of P. americana was previously reported to be similar to that of ACE inhibitors with decreased resorption of fluid from the distal convoluted tubule, resulting in natriuresis and decreased plasma volume in high salt-fed Wistar rats [22]. Similarly, an earlier report stated that A. sativum lowered BP by stimulating production of vascular gasotransmitter H 2 S and enhancing nitric oxide regulation leading to smooth muscle cell relaxation, dilatation and lowered BP [23].…”

Persea Americana Seeds Cause Ileal Smooth Muscle Relaxation Via Stimulation of α-1 Adrenoceptors

“…which are currently been explored as a viable therapeutic approach in management of heart failure [24]. The probable mechanism of antihypertensive effect of P. americana [12,14,27] may be explained by the above mechanisms, justifying the traditional management of hypertension with P. americana. Stimulation of α1adrenoceptors is of clinical benefit to hypertensive patients and this plant may be developed as a drug candidate for management of heart failure.…”