Biochemical effects of three commercial formulations of Bacillus thuringiensis (Agerin, Dipel 2X and Dipel DF) on Spodoptera littoralis larvae

Abstract

Effectiveness of commercial formulations of Bacillus thuringiensis var Kurstaki "Agerin, Dipel 2X and Dipel DF" on total protein and lipid contents were studied. Activities of detoxification and metabolic enzymes were also measured in treated and control larvae of cotton leafworm Spodoptera littoralis. Collected samples from treated larvae at intervals of 48 and 120 hrs post treatment were analyzed to assess the total protein and lipid contents as well as the enzymes activities. Significant reductions were observed in total protein content at 120 hrs larvae in all treatments compared with control. In all larvae treated with Agerin and Dipel DF the lipid content was significantly elevated after 48 and 120 hrs of treatment while Dipel 2X showed no significant difference in lipid content. However, fluctuated changes in the enzymes activity of treated larvae were found. We conclude that there are differences in biochemical effects between various commercial formulations of Bacillus thuringiensis kurstaki. In most cases Agerin was found more effective on S. littoralis than others.

Biological and Biochemical Effects of Bacillus thuringiensis, Serratia marcescens and Teflubenzuron and their Sequential Combined Effects on Cotton Leafworm, Spodoptera littoralis (Boisd.).

“…The results either of S. marcescens or Teflubenzuron were in agreement with those reported by Tolba (2006) when A. ipsilon pupae were treated either with S. marcescens or Flufenoxuron. On the other hand, the decreased activity in acid phosphatase due to B. thureinginsis treatment is similar to Kamel et al (2010) who found decrease in acid phosphatase activity of S. littoralis larvae after 48hrs post-treatment with B. thureinginsis commercial formulations (Agerin, Dipel 2x and Dipel DF ), whereas , it was increased after 120 hrs. for Agerin.…”

Some Toxicological and Physiological Aspects Induced by Camphor oil, Cinnamomum Camphora on the Cotton Leafworm, Spodoptera littoralis (Boisduval). (Lepidoptera: Noctuidae).

“…In addition, the decrease in total soluble protein might be due to the breakdown of protein into amino acids, so with the entrance of these amino acids to TCA cycle as keto acids, they will lead to supply energy for the insect. So, protein depletion in tissues may cause physiological mechanisms and might play a role in compensatory mechanisms under insecticidal stress, to provide intermediates to the Krebs cycle, by keeping free amino acids content in haemolymph (Nath et al, 1997 andKamel et al, 2010).…”

Biochemical studies of Bacillus Thuringiensis var.kurstaki, Serratia marcescns and Teflubenzurone on cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptea: Noctuidae)

“…kurstaki caused a significant reduction in the protein content of S.littoralis larvae. Kamel et al (2010) observed significant reduction in total protein content of S. littoralis larvae after treatment with B. thuringiensis var. kurstaki.…”