Biochemical Features and Virulence Gene Profiles of Non-O157/O26 Enterohemorrhagic &lt;i&gt;Escherichia coli&lt;/i&gt; Strains from Humans in the Yamaguchi Prefecture, Japan

Kameyama, Mitsuhiro; Yabata, Junko; Nomura, Yasuharu; Tominaga, Kiyoshi

doi:10.7883/yoken.jjid.2014.303

Cited by 4 publications

(4 citation statements)

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“…It has subsequently been detected in other DEC pathotypes, including EPEC, ETEC, and STEC (25)(26)(27). Although the role of EAST1 in human disease is unknown (28), we have designated E. coli that possess astA but no other identifiable pathogenic properties as EAST1EC.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Diarrheagenic <i>Escherichia coli</i> in Foods and Fecal Specimens Obtained from Cattle, Pigs, Chickens, Asymptomatic Carriers, and Patients in Osaka and Hyogo, Japan

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Prevalence of Diarrheagenic <i>Escherichia coli</i> in Foods and Fecal Specimens Obtained from Cattle, Pigs, Chickens, Asymptomatic Carriers, and Patients in Osaka and Hyogo, Japan

et al. 2017

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“…The astA gene encoding EAEC heat-stable enterotoxin 1 (EAST1) was initially detected in EAEC (Nataro and Kaper, 1998) and subsequently has been detected quite frequently in other DEC pathotypes including EPEC, ETEC, and EHEC (Kameyama et al, 2015;Sirikaew, 2014;Wang et al, 2013). Although the role of EAST1 in human disease still remains to be clarified, EAST1EC, defined as an E. coli possessing only the astA as a possible virulence gene, has been implicated in several outbreaks (Nishikawa et al, 1999;Zhou et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, antimicrobial resistance and multiple-locus variable-number tandem-repeat analysis profiles of diarrheagenic Escherichia coli isolated from different retail foods

Wang

Nakamura

Kage-Nakadai

et al. 2017

International Journal of Food Microbiology

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Diarrheagenic E. coli (DEC) isolates were recovered from local retail markets and the Osaka Municipal Central Wholesale Market in Japan. Retail food samples were collected for analysis in Osaka Japan from 2005 to 2008 and consisted of 32 beef, 28 pork, 20 poultry, 136 fish, 66 fruits and vegetables and 51 ready-to-eat (RTE) food samples. A total of 82 DEC strains were recovered from 64 (19%) food samples with the highest prevalence in poultry (100%, 20/20), followed by pork (54%, 15/28), beef (28%, 9/32), fruits and vegetables (12%, 8/66), fish (6.6%, 9/136) and RTE foods (5.9%, 3/51). Most of the strains belonged to E. coli possessing the enteroaggregative E. coli (EAEC) heat-stable enterotoxin 1 (EAST1) gene (EAST1EC; n=62, P<0.0001) and enteropathogenic E. coli (EPEC; n=16, P<0.01), whereas only 1 strain belonged to Shiga toxin-producing E. coli (STEC), 1 to EAEC and 2 to enterotoxigenic E. coli (ETEC) strains. Of the 82 DEC isolates, 22 O and 13H serogroups were detected, including some specific serogroups (O91, O103, O115, O119, O126, and O157) which have been associated with human diarrheal infections. Phylogenetic group A and B1 were predominant among the DEC isolates. Antimicrobial resistance to tetracycline was most common (49%), followed by nalidixic acid (28%), ampicillin (24%), sulfamethoxazole/trimethoprim (20%), and cephalothin (18%). All isolates were susceptible to aztreonam. Of the resistant strains, 44% (22/50) demonstrated resistance to >3 antimicrobial agents. Isolates resistant to >5 antimicrobials were only found in the meat samples, while isolates from the fruits and vegetables as well as RTE foods showed resistance to only 1 or 2 antimicrobial agents. Sixty one percent of EAST1EC, 56% of EPEC and all of the EAEC and ETEC were resistant to at least 1 antimicrobial agent. Multiple-locus variable-number tandem repeat analysis (MLVA) was used in this study for genotyping of DEC. The 82 isolates collected for this study showed 77 distinct MLVA profiles located among 3 branches. The Simpson's Index of Diversity (D) was 99.9% at its highest. The high diversity of these food strains would suggest their originating from a variety of sources and environments. In conclusion, retail food samples in Japan were contaminated with DEC; EAST1EC, a putative DEC, were detected at high rates in poultry, pork and beef. Isolates resistant to >3 antimicrobials were found only in raw meat and fish. Food animals may act as the reservoir for multi-resistant bacteria. Due to the finding that nearly 1/3 of EAST1EC strains were resistant to >3 antimicrobials, additional surveillance for EAST1EC should be initiated.

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“…A study from Switzerland recorded that the top four serogroups in their country were O103, O26, O91 and O80 [18]. Data from Korea showed O103, O26, O91 and O8 [19] and Japan reported O26, O111, O103, O121 and O145 [20] as their predominant serogroups. British Columbia, Canada (located west of Alberta) found their top serogroups to be O121, O26, O103, O117 and O111 [21].…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Non-O157 Shiga Toxin-Producing Escherichia coli in the Province of Alberta, Canada, from 2018 to 2021

2022

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Non-O157 serogroups contribute significantly to the burden of disease caused by Shiga toxin-producing Escherichia coli (STEC) and have been underrecognized by traditional detection algorithms. We described the epidemiology of non-O157 STEC in Alberta, Canada for the period of 2018 to 2021. All non-O157 STEC isolated from clinical samples were submitted for serotyping and qPCR targeting the stx1 and stx2 genes. A total of 729 isolates were identified. Increased detection occurred over the summer months, peaking in July. Patients 18 years and younger made up 42.4% of cases, with 31.1% in those 0–9 years of age. There was a slight female predominance (399/729, 54.7%) A total of 50 different serogroups were detected; the most common were O26 (30.3%), O103 (15.9%), O111 (12.8%), O121 (11.0%), O118 (3.3%) and O71 (2.9%). These six serogroups made up 76.2% of all isolates. In total, 567 (77.8%) were positive for stx1, 114 (15.6%) were positive for stx2 and 48 (6.6%) were positive for both stx1 and stx2. A wide variety of non-O157 serogroups have been detected in Alberta, with the most frequent serogroups differing from other locations. These results highlight the need for further characterization of their virulence factors and clinical impact.

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Biochemical Features and Virulence Gene Profiles of Non-O157/O26 Enterohemorrhagic <i>Escherichia coli</i> Strains from Humans in the Yamaguchi Prefecture, Japan

Cited by 4 publications

References 25 publications

Prevalence of Diarrheagenic <i>Escherichia coli</i> in Foods and Fecal Specimens Obtained from Cattle, Pigs, Chickens, Asymptomatic Carriers, and Patients in Osaka and Hyogo, Japan

Prevalence of Diarrheagenic <i>Escherichia coli</i> in Foods and Fecal Specimens Obtained from Cattle, Pigs, Chickens, Asymptomatic Carriers, and Patients in Osaka and Hyogo, Japan

Prevalence, antimicrobial resistance and multiple-locus variable-number tandem-repeat analysis profiles of diarrheagenic Escherichia coli isolated from different retail foods

Epidemiology of Non-O157 Shiga Toxin-Producing Escherichia coli in the Province of Alberta, Canada, from 2018 to 2021

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