Biochemical, haematological and morphological variations in juvenile<i>Clarias gariepinus</i>exposed to Carbendazim<sup>®</sup>fungicide

Nwani, CD; Omah, MC; Ivoke, Njoku; Ho, Nwamba; Ani, C. E.; Ogbonna, SU

doi:10.2989/16085914.2015.1014022

Cited by 16 publications

(15 citation statements)

References 38 publications

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“…To our knowledge the only study available concerning carbendazim effects on fish behaviour is the recent study conducted in juveniles of the african sharptooth catfish (Claria gariepinus). Fish exposed to sublethal concentrations of carbendazim (0.22 -0.43 mg/L) showed abnormal behavioural responses such as spiral swimming, hyperactivity, frequent surfacing to gulp water, erratic movement and loss of equilibrium (Nwani et al, 2015). Alteration of locomotor behaviour is very often linked with neurological impairment mediated by ChE inhibition, however this is not the case for carbendazim since this compound does not inhibit ChE as it is known from the literature (Kegley et al, 2014) and also confirmed in this work.…”

Section: Discussionmentioning

confidence: 99%

Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos

et al. 2016

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Carbendazim is a widely used broad spectrum benzimidazole fungicide; however, its effects to non-target aquatic organisms are poorly studied. The aim of this study was to investigate the toxic effects of carbendazim to zebrafish early life stages at several levels of biological organization, including developmental, biochemical and behavioural levels. The embryo assay was done following the OECD guideline 236 and using a concentration range between 1.1 and 1.8mg/L. Lethal and developmental endpoints such as hatching, edemas, malformations, heart beat rate, body growth and delays were assessed in a 96h exposure. A sub-teratogenic range (from 0.16 to 500μg/L) was then used to assess effects at biochemical and behavioural levels. Biochemical markers included cholinesterase (ChE), glutathione-S-transferase (GST), lactate dehydrogenase (LDH) and catalase (CAT) and were assessed at 96h. The locomotor behaviour was assessed using an automated video tracking system at 120h. Carbendazim (96h-LC50 of 1.75mg/L) elicited several developmental anomalies in zebrafish embryos with EC50 values ranging from 0.85 to 1.6mg/L. ChE, GST and LDH activities were increased at concentrations equal or above 4μg/L. The locomotor assay showed to be extremely sensitive, detecting effects in time that larvae spent swimming at concentrations of 0.16μg/L and thus, being several orders of magnitude more sensitive that developmental parameters or lethality. These are ecological relevant concentrations and highlight the potential of behavioural endpoints as early warning signs for environmental stress. Further studies should focus on understanding how the behavioural disturbances measured in these types of studies translate into fitness impairment at the adult stage.

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Section: Discussionmentioning

confidence: 99%

Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos

et al. 2016

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“…One main feature of thiophanate-methyl is its fast absorption on the surface of treated plant, coupled with an ability to spread rapidly and transformed into carbendazim by the effect of plant enzymes or sunlight, which is also responsible for the fungal activity. In addition to carcinogenesis of thiophanate-methyl that has been well reported (U.S.EPA 1999), some other studies have indicated other harmful effects including endocrine disruption and damaging of mammalian genetic system and metabolism (Capriglione et al 2011;Cardone 2012;Feki et al 2017;Nwani et al 2015;Salihu et al 2016;Singh et al 2016). In response to any undesirable impacts of pesticides, set of worldwide measures Editorial Responsibility: Lotfi Aleya Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11356-020-10186-4) contains supplementary material, which is available to authorized users.…”

Section: Introductionmentioning

confidence: 95%

Dissipation behavior of thiophanate-methyl in strawberry under open field condition in Egypt and consumer risk assessment

Malhat

Abdallah

Ahmed

et al. 2020

Environ Sci Pollut Res

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Thiophanate methyl is a broad-spectrum benzimidazole fungicide extensively applied in pre-and post-harvest, for the control of a wide range of fruit and vegetable pathogens. In the current work, the residue behavior of thiophanate methyl after application on strawberries and an estimation of the consumer dietary exposure was performed. Supervised field trials were conducted in Egypt (Qaluobiya Governorate) as to investigate the residue dynamics and terminal residues at different PHIs of thiophanate methyl and its metabolite carbendazim in strawberries under Egyptian conditions. For the measurement of residues in fruits, a QuEChERSbased protocol coupled with LC-MS/MS was optimized and successfully validated at 0.01 mg kg −1. The half-life (t 1/2) of thiophanate methyl in strawberries was estimated, and a dietary risk assessment was performed employing both FAO/WHO and EFSA approaches.

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“…Fish in their natural habitats are extremely sensitive to environmental pollutants. The HSI and CF are morphological indices of fish health [ 21 , 37 ]. The body weight of the exposed fish decreased but was not significantly different from that of the control fish in the present study.…”

Section: Discussionmentioning

confidence: 99%

Biomorphometric and hematobiochemical alterations in the juvenile african catfish Clarias gariepinus exposed to propranolol

Melefa,

Hinmikaiye,

Andong

et al. 2024

BMC Zool

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Propranolol (PRO) is a beta-blocker drug used for the treatment of anxiety, chest pain, migraine and tremors. The present study investigated whether sublethal concentrations of PRO have effects on the body condition, biochemistry, and hematology of Clarias gariepinus juveniles. The 96-h median lethal concentration (LC50) of the drug, which was established through an acute toxicity study, was 9.48 mg/L. Based on these values, the fish were exposed for 21 days to the control and sublethal concentrations of 1.90, 0.95, and 0.63 mg/L, which are equivalent to the 1/5th, 1/10th, and 1/20th of the LC50 of PRO, respectively. After 21 days of exposure, the fish were removed from the toxicant and kept in toxicant-free water for 7 days to recover. The standard length and body weight of each fish were measured after each exposure period. The condition factor (CF) and hepatosomatic index (HSI) were not significantly affected by the drug. The red blood cell (RBC) count, hemoglobin (Hb) count and packed cell volume (PCV) decreased from day 7 to 21 at the tested concentrations, while the white blood cell (WBC) count significantly increased. There were alterations in the mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and mean corpuscular hemoglobin (MCH) in the exposed groups compared to those in the control group. When neutrophil counts increased, the lymphocyte counts decreased, but the monocyte, basophil cell and eosinophil cell counts were not affected. Among the liver enzymes, only aspartate aminotransferase was significantly stimulated in the groups that were exposed to the drug. The protein and glucose levels of fish exposed to the drug decreased. Most of the studied parameters returned to their original values after the 7-day recovery period. The information provided in the current study will be helpful in the monitoring of PRO contamination in aquatic environments.

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Biochemical, haematological and morphological variations in juvenileClarias gariepinusexposed to Carbendazim^®fungicide

Cited by 16 publications

References 38 publications

Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos

Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos

Dissipation behavior of thiophanate-methyl in strawberry under open field condition in Egypt and consumer risk assessment

Biomorphometric and hematobiochemical alterations in the juvenile african catfish Clarias gariepinus exposed to propranolol

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Biochemical, haematological and morphological variations in juvenileClarias gariepinusexposed to Carbendazim®fungicide

Cited by 16 publications

References 38 publications

Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos

Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos

Dissipation behavior of thiophanate-methyl in strawberry under open field condition in Egypt and consumer risk assessment

Biomorphometric and hematobiochemical alterations in the juvenile african catfish Clarias gariepinus exposed to propranolol

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Biochemical, haematological and morphological variations in juvenileClarias gariepinusexposed to Carbendazim^®fungicide