Biochemical, histologic, and biomechanical characterization of native and decellularized flexor tendon specimens harvested from the pelvic limbs of orthopedically normal dogs

Balogh, Daniel G.; Biskup, Jeffery J.; O’Sullivan, M. Gerard; Scott, Ruth; Groschen, D.M.; Evans, Richard B.; Conzemius, Michael G.

doi:10.2460/ajvr.77.4.388

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…All DDFT allografts were obtained from a commercial source (Veterinary Transplant Services, Kent, Washington) with standard operating procedures regarding collection, processing, and storage of grafts as previously described . Whole DDFT tendons were decellularized by using a protocol based on a published method …”

Section: Methodsmentioning

confidence: 99%

Long‐term arthroscopic assessment of intra‐articular allografts for treatment of spontaneous cranial cruciate ligament rupture in the dog

Biskup

Conzemius

2020

Veterinary Surgery

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Objective: To assess the 12-month survival of intra-articular, decellularized allografts for the treatment of cranial cruciate ligament disease (CCLD) in dogs. Study design: Prospective case series.Animals: Twenty client-owned dogs with spontaneous CCL rupture. Methods: Affected stifles were treated solely by implantation of an intra-articular, decellularized, deep digital flexor tendon allograft. Functional outcome was evaluated at baseline and at 6 and 12 months after surgery with the Liverpool Osteoarthritis for Dogs questionnaire and force platform gait analysis. At 12 months after surgery, dogs underwent arthroscopy to visually assess the allografts.Results: Scores on owner questionnaires (improved 62.5%; P = .005) and ground reaction forces (improved 34.4%; P = .017) were improved in all dogs 12 months after surgery. Dogs with an intact graft (IG) were more likely to have a successful outcome compared with dogs with a torn graft (P = .011).According to arthroscopy, 45% of grafts seemed to survive at 12 months after surgery. Conclusion: About half of intra-articular allografts survived 12 months after implantation in CCL-deficient stifles. Dogs with IG had successful outcomes at 12 months after surgery according to assessment by force platform gait analysis. Clinical significance: These results preclude immediate clinical application but provide evidence to justify further investigation of intra-articular grafts as a repair option for CLLD.

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Section: Methodsmentioning

confidence: 99%

Long‐term arthroscopic assessment of intra‐articular allografts for treatment of spontaneous cranial cruciate ligament rupture in the dog

Biskup

Conzemius

2020

Veterinary Surgery

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“…The ligaments were wrapped in sterile gauze, soaked in phosphate‐buffered saline, and frozen at −80°C. Whole tendons were then decellularized using a protocol derived from previous publications …”

Section: Methodsmentioning

confidence: 99%

“…Medial femoral transfix reconstruction with a DDFT allograft, tibial spiked washers, and an interference screw, reproduced the salient mechanical properties of the native CCL . Finally, a study evaluating the histological, biochemical, and mechanical effects of decellularization on DDFTs reported unchanged mechanical properties in spite of a decrease in DNA content of the graft …”

Section: Introductionmentioning

confidence: 99%