Biochemical identification of bacteria by replicator methods on agar plates

Lennox, V.A.; Ackerman, V.P.

doi:10.3109/00313028409084736

Cited by 14 publications

(6 citation statements)

References 16 publications

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“…The biochemical studies of P. rettgeri were performed on MicroScan Walk-Away ® [27,28]. Biochemical reactions patterns were carried out using 32 biochemicals viz.…”

Section: Biochemical Studiesmentioning

confidence: 99%

Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri

Trivedi¹,

Shettigar²,

Jana³

2015

Mol Biol

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Providencia rettgeri (P. rettgeri) is a clinically significant Gram-negative bacterium of genus Providencia, and commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular infections. Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence. The samples of P. rettgeri (ATCC 9250) were divided into three groups: Gr.I (control), Gr.II (treatment, revived), and Gr.III (treatment, lyophilized). The Gr.II and III were treated with Mr. Trivedi's biofield, and then subsequently characterized for antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotype numbering. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of P. rettgeri with other bacterial species. The treated cells of P. rettgeri showed an alteration in susceptibility of about 50% and 53.3% tested antimicrobials of Gr.II on day 5 and 10, respectively; and 53.3% of tested antimicrobials of Gr.III on day 10. MIC results showed a significant decrease in MIC values of 53.1, 56.3, and 56.3% antimicrobials in Gr.II on day 5, Gr.II on day 10, and Gr.III on day 10, respectively, as compared to control. The significant changes in biochemical reactions and biotype numbers were also observed in all the treated groups of P. rettgeri. Based on nucleotides homology and phylogenetic analysis the P. rettgeri was found to be Proteus mirabilis (GenBank Accession Number: AY820623) and nearest homolog species was found to be Proteus vulgaris (Accession No. DQ499636). These findings suggest that biofield treatment can prevent the emergence of absolute resistance of existing antimicrobials to P. rettgeri.

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“…The biochemical studies of P. rettgeri were performed on MicroScan Walk-Away ® [27,28]. Biochemical reactions patterns were carried out using 32 biochemicals viz.…”

Section: Biochemical Studiesmentioning

confidence: 99%

Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri

Trivedi¹,

Shettigar²,

Jana³

2015

Mol Biol

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“…There was strong hybridization with clones that contained known homologous genes, including the original clinical isolate E. coli VOL. 28,1985 on May 10, 2018 by guest http://aac.asm.org/ 100 GROOT OBBINK ET AL. VA292 and its transconjugant E. coli E292.…”

Section: Resultsmentioning

confidence: 99%

“…Susceptibilities to antibiotics were determined by agar dilution (43), and identification of the organisms was carried out by a replica plating method for biochemical analysis (28). Standard strains and plasmids are listed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Construction of a gentamicin resistance gene probe for epidemiological studies

Obbink¹,

Ritchie²,

Cameron³

et al. 1985

Antimicrob Agents Chemother

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A 7.7-kilobase BamHI fragment was cloned from the transconjugant of a clinical isolate of Escherichia coli containing a 120-kilobase multiresistance IncC plasmid. The recombinant plasmid conferred resistance to kanamycin, gentamicin, tobramycin, sulfamethoxazole, and trimethoprim. This clone was used to generate a series of subclones from which a 2.0-kilobase BamHI-HindIII probe containing a gentamicin 2''-O-adenylyltransferase [AAD(2'')] gene was obtained. This probe hybridized specifically in both colony and Southern hybridizations with the AAD(2'') gene but not with other resistance genes, including other aminoglycoside-modifying genes, or with a reference IncC plasmid lacking the AAD(2'') gene. The AAD(2'') gene may be part of a transposon, since hybridization occurred with both nonconjugative plasmids and the chromosome in some isolates.

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“…The received 16S rDNA sequences data were aligned and compared with the sequences stored in Gene Bank database of National Center for Biotechnology Information (NCBI) using the algorithm BLASTn program. Finally, the multiple sequence alignment/phylogenetic tree was constructed with help of MEGA 3.1 software utilizing neighbor joining method [29,30].…”

Section: S Rdna Gene Sequencingmentioning

confidence: 99%

Evaluation of Phenotyping and Genotyping Characteristic of Shigella sonnei after Biofield Treatment

Trivedi¹,

Shettigar²

2015

J Biotechnol Biomater

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Shigella sonnei (S. sonnei) is a non-motile, rod shape, clinically significant, Gram-negative bacterium. It is commonly associated with dysentery (shigellosis). Recently, resistance to third and fourth generation cephalosporins and fluoroquinolones has been reported in S. sonnei. In the present study, we assessed the effect of biofield treatment on phenotyping and genotyping characteristic of S. sonnei (ATCC 9290). The lyophilized samples of S. sonnei were divided in three groups (G): G-I (control, revived), G-II (treatment, revived), and G-III (treatment, lyophilized). All these groups (control and biofield treated) were analyzed against antimicrobial susceptibility, biochemical reactions, and biotype number. The 16S rDNA sequencing was carried out to establish the phylogenetic relationship of S. sonnei with different bacterial species. The treated cells of S. sonnei exhibited an alteration of 3.33%, 10%, and 23.33% of total 30 tested antimicrobials in susceptibility assay for G-II on day 5 and 10 and G-III on day 10, respectively as compared to control. The treated cells of S. sonnei showed a significant change of about 12.12%, 12.12%, and 57.58% biochemical reactions out of 33 tests in treated groups of G-II on day 5 and 10 and G-III on day 10, respectively. The biotype number was also changed in treated samples of S. sonnei. Based on nucleotide homology sequences and phylogenetic analysis, the nearest homolog species of S. sonnei (GenBank Accession Number: EU009190) was identified as Shigella flexneri (EF643608). These results revealed that biofield treatment can prevent the absolute resistance in microbe against the existing antimicrobials.

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Biochemical identification of bacteria by replicator methods on agar plates

Cited by 14 publications

References 16 publications

Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri

Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri

Construction of a gentamicin resistance gene probe for epidemiological studies

Evaluation of Phenotyping and Genotyping Characteristic of Shigella sonnei after Biofield Treatment

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