Biochemical identification of viruses causing the 1981 outbreaks of foot and mouth disease in the UK

King, Andrew M. Q.; Underwood, B. O.; McCahon, D.; Newman, John W.; Brown, F.

doi:10.1038/293479a0

Cited by 163 publications

(57 citation statements)

References 7 publications

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“…In both studies, the virus strains analyzed do not represent all areas of the world where dengue 1 and 2 virus infections occur and, therefore, it is likely that additional genotypic varieties exist. The data suggest that the evolution of dengue virus within a specific geographic region is much slower than that observed for other non-insect-transmitted RNA viruses (44)(45)(46)(47)(48)(49)(50), and, therefore, it may be postulated that the mosquito vector plays an important role in selecting viruses within populations conferring genetic stability on the popUlation of virus transmitted. A second observation relating severity of disease with virus genotype suggests there is no direct relationship between fingerprint type and disease severity; mild to severe disease with hemorrhagic symptoms and shock have been associated with viruses having almost identical fingerprint patterns (D.W. Trent, personal communication).…”

Section: Molecular Epidemiologymentioning

confidence: 87%

Current Research on Dengue

Gubler

1987

Advances in Soil Science

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Section: Molecular Epidemiologymentioning

confidence: 87%

Current Research on Dengue

Gubler

1987

Advances in Soil Science

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“…Sequence heterogeneity among individual cloned viruses recovered from a single animal has been documented [77,124,196]. Genetic and phenotypic heterogeneity has been found in viral populations recovered upon infection of swine with plaque-purified viruses [45,46].…”

Section: Variability In Cell Culturementioning

confidence: 98%

“…FMDV can be mechanically disseminated by animals, farmers, farming equipment, and during animal transport [36]. Long-distance, airborne transmission has also been documented [84,124]. The initial virus multiplication usually takes place in the pharynx epithelium, producing primary vesicles, or "aphthae" [43].…”

Section: The Virus and The Diseasementioning

confidence: 99%

Foot-and-mouth disease virus: a long known virus, but a current threat

et al. 2001

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-Foot-and-mouth disease virus (FMDV) was the first animal virus identified. Since then, FMDV has become a model system in animal virology and a considerable amount of information on its structure, biology and vaccinology has been obtained. However, the disease that this virus produces (FMD) still constitutes one of the main animal health concerns. In this review, we have attempted to summarise the state of the knowledge in different basic and applied areas of FMDV research, with emphasis on those aspects relevant to the control of the disease. FMDV / structure / immunity / vaccine / variability / diagnosisRésumé -Le virus de la fièvre aphteuse : un virus connu de longue date, qui demeure une menace. Le virus de la fièvre aphteuse a été le premier virus animal identifié. Depuis lors, il est devenu un système modèle en virologie animale, et une quantité importante d'informations sur sa structure, sa biologie et sa vaccinologie a été obtenue. Cependant la maladie provoquée par ce virus constitue encore une inquiétude majeure en santé animale. Dans cette revue, nous avons tenté de résumer l'état des connaissances dans différents domaines de recherche, à la fois fondamentaux et appliqués, sur le virus de la fièvre aphteuse, en mettant l'accent sur les aspects relatifs au contrôle de la maladie. virus de la fièvre aphteuse / immunité / vaccin / variabilité / diagnostic

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“…At least some of the substitutions fixed in cell culture were biologically relevant, since the passaged virus showed a larger yield of infectious particles per cell than the parental virus (Sobrino et al, 1983). Genetic and antigenic heterogeneity have been documented in field isolates of FMDV (King et al, 1981 ;Rowlands et aL, 1983;Piccone et al, 1988 ;Mateu et al, 1988). The antigenic diversity of the virus limits the geographical area in which a given vaccine can be successfuly applied.…”

Section: Introductionmentioning

confidence: 99%

Selection of Antigenic Variants of Foot-and-Mouth Disease Virus in the Absence of Antibodies, as Revealed by an in situ Assay

Díez

Mateu

Domingo

1989

Journal of General Virology

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SUMMARYAntigenic variants of foot-and-mouth disease virus (FMDV) of serotype C (isolate C-S8cl) were selected upon serial passage of the virus in cell culture in the absence of anti-FMDV antibodies. The variants rose from frequencies of < 10 -2 in the initial plaque-purified FMDV C-S8cl preparation, to 0.1 to 1 in three passaged populations. The proportion of antigenic variants was quantified using a new in situ plaque immunotest. A nitrocellulose filter is applied to the agar overlay of a FMDV plaque assay, and allows recovery of infectious virus from individual plaques. A second filter is placed directly on the cell monolayer and binds enough virus to permit colorimetric visualization of plaques by an enzyme-linked assay using monoclonal antibodies (MAbs). Either all or a fraction of plaques from passaged FMDV failed to react with MAb 4G3, an antibody that recognizes an epitope located within residues 144 to 150 of capsid protein VP1. Some variants rapidly dominated the viral population, and others were maintained at low levels. RNA from unreactive viruses included mutations that resulted in amino acid substitutions at the epitope recognized by MAb 4G3. We discuss models for the selection of antigenic variants of FMDV in the absence of antibodies, and implications for the antigenic diversification of RNA viruses.

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Biochemical identification of viruses causing the 1981 outbreaks of foot and mouth disease in the UK

Cited by 163 publications

References 7 publications

Current Research on Dengue

Current Research on Dengue

Foot-and-mouth disease virus: a long known virus, but a current threat

Selection of Antigenic Variants of Foot-and-Mouth Disease Virus in the Absence of Antibodies, as Revealed by an in situ Assay

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