Biochemical Indices of Blood Serum of Rubella-Resistant Carp Breed at the End of the Feeding Period

Abstract

Biochemical parameters of blood serum in resistant and susceptible to rubella pathogens groups of carp at the end of the feeding period are studied. The material was taken from 6 individuals of the Angelina scaly rubella-resistant breed kept at the experimental pond base "Sunoga" of the Papanin Institute for Biology of Inland Waters RAS, Yaroslavl oblast. For comparison, we used same-age carps from the fish farm "Kirya", Chuvash Republic, susceptible to rubella pathogens: 8 specimens of scaly and 8 mirror carps. Blood samples were taken from the caudal vein of the fish under study. The tube with blood was left at room temperature. After coagulation, the serum was taken with a fine needle syringe and transferred to an Eppendorf tube. Serum samples were frozen prior to testing. The analyzed individuals of the Angelina rubella-resistant breed differ from the scaly and mirror carp in high levels of total protein, albumins, glucose, urea, alkaline phosphatase, triglycerides, cholesterol and low levels of lipids and alanine aminotransferase. A comparative analysis with previously obtained data after wintering shows differences between the autumn and spring data. In the Angelina breed, the levels of most indicators significantly increase during the fattening period. The content of total protein increases several times, mainly due to globulins, glucose, urea, alkaline phosphatase, triglycerides. Thus, the metabolic processes aimed at the accumulation of proteins, stored lipids and carbohydrates increase in the organism of rubella-resistant carps. This is probably due to some specific characteristics of the Angelina breed, which allows the fish organism to successfully resist an infection with rubella pathogens in spring. The results of this study can be used in breeding for immunity resistance and for assessing the health status of fish in aquaculture.

Some Indicators of Nonspecific Immunity of Various Breeding Groups of Carp in Different Periods of the Annual Cycle

“…The study of the indices of nonspecific immunity in rubella-resistant carp will make it possible to understand the mechanisms that ensure the immunity of fish to infectious diseases. Previously, the authors have shown differences in some immunobiochemical parameters between rubella-resistant carp and breeds susceptible to disease (Mikryakov et al, 2019a(Mikryakov et al, , 2019bSuvorova et al, 2019). It was found that individuals of the rubellaresistant breed differ from carp of other breeding groups in the proportions of the content of various forms of leukocytes; the intensity of leukopoiesis; the level of protein, glucose, and total lipids; oxidative processes; and antioxidant protection.…”