Biochemical insight into pseudouridine synthase 7 (PUS7) as a novel interactor of sirtuin, SIRT1

Dalal, Sayli; Deshmukh, Prashant; Unni, Sruthi; Padavattan, Sivaraman; Padmanabhan, Balasundaram

doi:10.1016/j.bbrc.2019.08.097

Cited by 2 publications

(4 citation statements)

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“…Previous investigation has highlighted PUS7 directly interacts with SIRT1 using biochemical approaches 30 . To verify the interaction between PUS7 and SIRT1, Co‐IP assays were conducted in HCT116 cells.…”

Section: Resultsmentioning

confidence: 99%

“…Previous investigation has highlighted PUS7 directly interacts with SIRT1 using biochemical approaches. 30 To verify the interaction between PUS7 and SIRT1, Co-IP assays were conducted in HCT116 cells. Western blot confirmed that PUS7 and SIRT1 could be coprecipitated with each other (Figure 5A).…”

Section: T a B L E 1 Correlation Between Pus7 Expression And The Clin...mentioning

confidence: 99%

“…Moreover, aberrant SIRT1 expression was associated with CRC proliferation, 25 metastasis, 26,27 stem‐cell‐like features, 28 and senescence‐like growth arrest 29 . Additionally, PUS7 was discovered to directly interact with SIRT1 via in silico and biochemical approaches 30 . In addition, SIRT1 plays a crucial role in stimulating the Wnt/β‐catenin pathway in many tumor types 31–33 .…”

Section: Introductionmentioning

confidence: 99%

“…29 Additionally, PUS7 was discovered to directly interact with SIRT1 via in silico and biochemical approaches. 30 In addition, SIRT1 plays a crucial role in stimulating the Wnt/β-catenin pathway in many tumor types. [31][32][33] However, the role of the interaction between PUS7 and SIRT1 in the development of CRC is unknown.…”

mentioning

confidence: 99%

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PUS7 promotes the proliferation of colorectal cancer cells by directly stabilizing SIRT1 to activate the Wnt/β‐catenin pathway

Zhang

Fei

Zhao

et al. 2022

Molecular Carcinogenesis

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Pseudouridine synthase 7 (PUS7) may play key roles in cancer development. However, few studies have been conducted in this area. In the present study, we explored the function and potential mechanisms of PUS7 in colorectal cancer (CRC) progression. We found that PUS7 had higher expression in CRC tissues and cell lines. Clinically, high expression of PUS7 was associated with an unfavorable prognosis for CRC patients. Functionally, knockdown of PUS7 suppressed the proliferation of CRC cells in vitro and inhibited tumorigenicity in vivo. Mechanistically, RNA sequencing and coimmunoprecipitation (Co‐IP) indicated that PUS7 exhibited oncogenic functions through the interaction of Sirtuin 1 (SIRT1) and activated the Wnt/β‐catenin signaling pathway. Thus, our findings suggest that PUS7 promotes the proliferation of CRC cells by directly stabilizing SIRT1 to activate the Wnt/β‐catenin pathway.

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Section: Resultsmentioning

confidence: 99%

Section: T a B L E 1 Correlation Between Pus7 Expression And The Clin...mentioning

confidence: 99%