Biochemical investigation of cholinesterases and carboxylesterases from the cotton bollwormHeliothis armigera

Kulieva, A. M.; Dalimov, D. N.; Dorenskaya, G. M.; Charieva, O. V.; Rozengart, V. I.; Kugusheva, L. I.; Moralev, S. N.; Babaev, B. N.; Abduvakhabov, A. A.

doi:10.1007/bf00638435

Cited by 2 publications

(1 citation statement)

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“…Surprisingly, to overcome the toxicity of gossypol, Helicoverpa armigera has developed a powerful detoxification system comprising detoxification enzymes such as CarE [25,26]. CarEs are α/β hydrolase proteins [27,28]. These are important detoxifying enzymes in insects and play an important role in the metabolism of toxic plant chemosensitive substances [29,30].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Free Gossypol by Helicoverpa armigera Carboxylesterase Expressed in Pichia pastoris

Zhang

Yang

Huang

et al. 2022

Toxins

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Gossypol is a polyphenolic toxic secondary metabolite derived from cotton. Free gossypol in cotton meal is remarkably harmful to animals. Furthermore, microbial degradation of gossypol produces metabolites that reduce feed quality. We adopted an enzymatic method to degrade free gossypol safely and effectively. We cloned the gene cce001a encoding carboxylesterase (CarE) into pPICZαA and transformed it into Pichia pastoris GS115. The target protein was successfully obtained, and CarE CCE001a could effectively degrade free gossypol with a degradation rate of 89%. When esterase was added, the exposed toxic groups of gossypol reacted with different amino acids and amines to form bound gossypol, generating substances with (M + H) m/z ratios of 560.15, 600.25, and 713.46. The molecular formula was C27H28O13, C34H36N2O6, and C47H59N3O3. The observed instability of the hydroxyl groups caused the substitution and shedding of the group, forming a substance with m/z of 488.26 and molecular formula C31H36O5. These properties render the CarE CCE001a a valid candidate for the detoxification of cotton meal. Furthermore, the findings help elucidate the degradation process of gossypol in vitro.

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