Biochemical investigations on peroxidase contents of male and female inflorescences of date palm (Phoenix dactylifera L.)

Qacif, N.; Baaziz, M.; Bendiab, K.

doi:10.1016/j.scienta.2007.07.015

Cited by 22 publications

(9 citation statements)

References 13 publications

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“…Therefore, we can keep considering guaiacol as being the best POD substrate for cotton cell suspension. These results are in accordance with those of some authors, which reported that guaiacol is the best commonly used substrate for plant peroxidase activity assay [20,21,[61][62][63].…”

Section: Substrate Specificitysupporting

confidence: 93%

Purification and Characterization of Cell Suspensions Peroxidase from Cotton (Gossypium hirsutum L.)

Kouakou

Dué

Kouadio

et al. 2008

Appl Biochem Biotechnol

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Two peroxidases, cPOD-I and rPOD-II, have been isolated and purified from cotton cell suspension and their biochemical characteristics studied. rPOD-II from R405-2000, a non-embryogenic cultivar, has higher activity than cPOD-I derived from Coker 312, which developed an embryogenic structure. The cPOD-I and rPOD-II had molecular mass of 39.1 and 64 kDa respectively, as determined by SDS-PAGE. Both enzymes showed high efficiency of interaction with the guaiacol at 25 mM. The optimal pH for cPOD-I and rPOD-II activity was 5.0 and 6.0, respectively. The enzyme had an optimum temperature of 25 degrees C and was relatively stable at 20-30 degrees C. The isoenzymes were highly inhibited by ascorbic acid, dithiothreitol, sodium metabisulfite, and beta-mercaptoethanol. Their activities were highly enhanced by Al(3+), Fe(3+), Ca(2+), and Ni(2+), but they were moderately inhibited by Mn(2+) and K(+). The enzyme lost 50% to 62% of its activity in the presence of Zn(2+) and Hg(2+).

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Section: Substrate Specificitysupporting

confidence: 93%

Purification and Characterization of Cell Suspensions Peroxidase from Cotton (Gossypium hirsutum L.)

Kouakou

Dué

Kouadio

et al. 2008

Appl Biochem Biotechnol

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“…There are no easily distinguishable sex chromosomes in date palm, despite some cytological evidence that they exist 5 . As biochemical studies have yielded little insight into how to identify the genders of immature plants 6 , the identification of DNA sequences or sequence polymorphisms that are gender specific offers a promising alternative to efficiently determine date palm gender.…”

Section: A R T I C L E Smentioning

confidence: 99%

De novo genome sequencing and comparative genomics of date palm (Phoenix dactylifera)

et al. 2011

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Date palm is one of the most economically important woody crops cultivated in the Middle East and North Africa and is a good candidate for improving agricultural yields in arid environments. Nonetheless, long generation times (5-8 years) and dioecy (separate male and female trees) have complicated its cultivation and genetic analysis. To address these issues, we assembled a draft genome for a Khalas variety female date palm, the first publicly available resource of its type for a member of the order Arecales. The ~380 Mb sequence, spanning mainly gene-rich regions, includes >25,000 gene models and is predicted to cover ~90% of genes and ~60% of the genome. Sequencing of eight other cultivars, including females of the Deglet Noor and Medjool varieties and their backcrossed males, identified >3.5 million polymorphic sites, including >10,000 genic copy number variations. A small subset of these polymorphisms can distinguish multiple varieties. We identified a region of the genome linked to gender and found evidence that date palm employs an XY system of gender inheritance.

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“…Biochemical studies give in a little information for sex identification of immature plants and gender specific DNA sequences offer a more capable and efficient method for gender determination (Qacif et al 2007 ). Various efforts had been made to comprehend the genetic basis of sex evaluation of plants at early stages of development with molecular tools (Biffi et al 1995 ; Hormaza et al 1994 ).…”

Section: Introductionmentioning

confidence: 99%

Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers

et al. 2016

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Microsatellite markers containing simple sequence repeats (SSRs) are a valuable tool for genetic analysis. Date palm is a dioecious and slow flowering and is very difficult to identify the gender of the trees until it reaches the reproductive age (5–10 years). A total of 12 microsatellite primers were used with 30 date palm samples, 14 parents (8 male + 6 females) and 16 progeny (developed from parents breeding) which showed that microsatellites were highly polymorphic, having a great number of alleles. A total of 124 alleles were characterized in 12 SSR loci. On average, there are 9.08 alleles per locus, with a range from 5 to 16 alleles, for primers mpdCIR15 and mpdCIR57, respectively. These primers produced 15 polymorphic loci specifically in male date palm samples and the seedlings harboring the unique fragments were further characterized as male plants. Increasingly, 38.46 % of these loci were scored as homozygous alleles while 61.53 % heterozygous allelic loci were determined. Primer mpdCIR48 produced a specific locus (250/250) in all male samples whereas the same locus was absent in female samples. Similarly, a locus of 300/310 bp reoccurred in 5 date palm male samples using marker DP-168 which indicated that these are the promising candidate marker to detect the sex in date palm seedlings at early stage. The data resulted from combination of 12 primers enabled the 16 seedling samples progeny (developed from parents breeding) of date palm cultivars to divide into two groups i.e., male and female regarding their sex expression comparative to the parents (male + female) using the principle coordinate analysis.

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Biochemical investigations on peroxidase contents of male and female inflorescences of date palm (Phoenix dactylifera L.)

Cited by 22 publications

References 13 publications

Purification and Characterization of Cell Suspensions Peroxidase from Cotton (Gossypium hirsutum L.)

Purification and Characterization of Cell Suspensions Peroxidase from Cotton (Gossypium hirsutum L.)

De novo genome sequencing and comparative genomics of date palm (Phoenix dactylifera)

Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers

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