Biochemical Markers and Enzyme Assays for Herbicide Mode of Action and Resistance Studies

Dayan, Franck E.; Owens, Daniel K.; Corniani, N.; Silva, Ferdinando Marcos Lima; Watson, Susan B.; Howell, J. F.; Shaner, Dale L.

doi:10.1614/ws-d-13-00063.1

Cited by 128 publications

(125 citation statements)

References 67 publications

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“…Reversible and irreversible changes in plant metabolism can be used as bioindicators for the action of xenobiotics (Boutin et al, 2014). Some of the biomarkers used in biomonitoring are the changes in the structure of nucleic acids (Wang et al, 2016), chlorophyll a fluorescence, gas exchange (Duke et al, 2003;MacFarlane, 2003), chloroplastid pigment content (Prasad et al, 2015), metabolites, proteins (Schrübbers et al, 2014;Dayan et al, 2015;Hattab et al, 2016), and the occurrence of chlorosis and necrosis in leaves (De-Temmerman et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Pouteria torta: a native species of the Brazilian Cerrado as a bioindicator of glyphosate action

et al. 2017

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In Brazil, the expansion of agricultural activity and the associated indiscriminate use of herbicides such as glyphosate is directly related to the loss of biodiversity in the Cerrado. The identification of plant species as bioindicators of herbicide action, especially species native to the area, can help in monitoring the impacts of xenobiotics in the remaining Cerrado. Thus, this study was designed to evaluate the possible use of the native Cerrado species Pouteria torta as a bioindicator of glyphosate action via changes in physiological performance. At 16 months after sowing, the effect of glyphosate was evaluated by applying the following doses: 0 (control), 25, 50, 100, 200, 400, 800, and 1200 g a.e. ha -1 . In response to glyphosate, P. torta exhibited reductions in photosynthesis and chloroplastid pigment content, as well as accumulation of shikimic acid and the occurrence of chlorosis and necrosis. These changes demonstrate the high sensitivity of P. torta to glyphosate and its potential for use as a bioindicator of this herbicide.Keywords: phytotoxicity, photosynthetic processes, biomarker, guapeva, herbicide. Pouteria torta: espécie native do Cerrado Brasileiro como bioindicadora da ação do glyphosate ResumoNo Brasil, a expansão da atividade agrícola, aliada a utilização indiscriminada de herbicidas como o glyphosate, possui relação direta com a perda da biodiversidade no Cerrado. A identificação de espécies vegetais bioindicadoras da ação de herbicidas, particularmente as nativas do Cerrado, pode auxiliar em processos de monitoramento dos impactos desse xenobiótico nas remanescentes do Cerrado. Assim, este estudo foi projetado para avaliar o possível uso de Pouteria torta, espécie nativa do cerrado, como bioindicadora da ação do glyphosate via mudanças na sua performance fisiológica. Após 16 meses de semeadura, o efeito do glyphosate foi avaliado quando aplicadas as seguintes doses: 0 (controle), 25, 50, 100, 200, 400, 800 e 1200 g e. a. ha -1 . Em reposta ao glyphosate, as plantas de P. torta apresentaram redução na sua performance do processo fotossintético e no conteúdo de pigmentos cloroplastídicos, além do acúmulo de ácido chiquímico e da ocorrência de cloroses e necroses. Essas alterações demonstram a alta sensibilidade de P. torta ao glyphosate, o que potencializa a sua utilização como bioindicadora da ação desse herbicida.

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Section: Introductionmentioning

confidence: 99%

Pouteria torta: a native species of the Brazilian Cerrado as a bioindicator of glyphosate action

et al. 2017

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“…The quantification of shikimic acid in glyphosate-treated plants has been used as metabolic marker of glyphosate sensitivity (Dayan et al, 2015). The shikimate pathway consumes ≥30% of carbon fixed through the Calvin cycle (Maeda and Dudareva, 2012) and the inhibition of EPSPS causes a non-regulated carbon flux into the shikimate pathway through induction of the expression of 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase, the first enzyme of this metabolic pathway (Zabalza et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The inhibition of EPSPS triggers the accumulation of shikimate, a substrate of the enzyme. Therefore, the effect of glyphosate on plants can be easily measured by monitoring the accumulation of this substrate of EPSPS (Dayan et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Resistance to Glyphosate in Lolium perenne from Argentina

et al. 2017

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In Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.

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“…Samples were placed in falcon tubes with 50 mL of water acidified with hydrochloric acid (pH 3.5) and placed in an ultrasound bath for 60 min. The ammonia content of the solution was assessed using a spectrophotometer (Cintra 40, GBC Scientific Equipment Ltd.) according to published methods (WENDLER; BARNISKE; WILD, 1990;DAYAN et al, 2015).…”

Section: Study Evaluationsmentioning

confidence: 99%

Variation in the Sensitivity of Wandering Jew Plants to Glufosinate Ammonium

et al. 2017

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-This study aimed to identify the response of wandering jew (Commelina benghalensis L.) plants to different doses of glufosinate ammonium and the sensitivity of plants populations to the herbicide. Two studies were conducted, both in a greenhouse, and were repeated at different times. In the first study, two experiments were conducted to examine the dose-response curve using seven different doses of the glufosinate ammonium herbicide (0, 50, 100, 200, 400, 800, and 1600 g a.i. ha -1 ) with four replicates each. In the second study, which examined the range in sensitivity of wandering jew plants to glufosinate ammonium, 26 plants were sprayed with a dose of 200 g a.i. ha -1 herbicide. Visual assessments of percent injury and measurements of leaf tissue ammonium content were conducted. The use of untreated wandering jew control plants allowed for the correlation of glufosinate ammonium treatment with the ammonium concentrations in treated plant tissues; the ammonium concentration increased as a function of herbicide application, albeit not linearly with the dose. Ammonium content varied among individuals of the wandering jew plant population.Keywords: Ammonia. Commelina benghalensis L.. Glutamine synthetase. Weed. VARIAÇÃO DA SENSIBILIDADE DE PLANTAS DE TRAPOERABA AO AMÔNIO GLUFOSINATERESUMO -Esse trabalho teve como objetivo identificar a resposta de plantas de trapoeraba (Commelina benghalensis L.) a diferentes doses de amônio glufosinate e a sensibilidade de uma população de plantas ao herbicida. Foram realizados dois estudos, ambos implantados em casa de vegetação e repetidos em diferentes momentos. No primeiro, referente à curva de dose-resposta, realizou-se dois experimentos, tendo como tratamentos sete doses do herbicida amônio glufosinate (0; 50; 100; 200; 400; 800; 1600 g i.a. ha -1 ), com quatro repetições cada. Já no segundo, referente à variação da sensibilidade da trapoeraba ao amônio glufosinate, 26 plantas foram pulverizadas com a dose de 200 g i.a. ha -1 do herbicida. Realizou-se avaliações visuais de injúria e análises do teor de amônia nos tecidos foliares. O controle de plantas de trapoeraba pelo amônio glufosinate pode ser correlacionado ao teor de amônia nos tecidos vegetais, que aumenta em função da aplicação do herbicida, porém não de forma linear em função da dose. Houve variabilidade quanto ao teor de amônia entre indivíduos de uma população de trapoeraba.Palavras-chave: Amônia. Commelina benghalensis L.. Glutamina sintetase. Planta daninha.

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Biochemical Markers and Enzyme Assays for Herbicide Mode of Action and Resistance Studies

Cited by 128 publications

References 67 publications

Pouteria torta: a native species of the Brazilian Cerrado as a bioindicator of glyphosate action

Pouteria torta: a native species of the Brazilian Cerrado as a bioindicator of glyphosate action

Mechanism of Resistance to Glyphosate in Lolium perenne from Argentina

Variation in the Sensitivity of Wandering Jew Plants to Glufosinate Ammonium

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