Biochemical Markers of Oxidative Stress in Saudi Women with Recurrent Miscarriage

Abstract

This study was undertaken to investigate the antioxidant/oxidant status in recurrent miscarriage patients. Antioxidants including glutathione peroxidase (GPx), catalase (CAT), glutathione reductase (GR), reduced glutathione (GSH) and selenium (Se), as well as the oxidants hydrogen peroxide (H2O2), oxidised glutathione (GSSG) and lipid peroxidation were assayed in plasma, whole blood and placental tissue of non-pregnant women (NP), healthy pregnant women (HP), and recurrent miscarriage (RM) patients. Results indicated that all antioxidant activities and levels in plasma and whole blood of HP women were consistently moderately lower, and much more significantly lower in RM patients when both were compared to those seen in NP women (P<0.05 and P<0.001, respectively). Furthermore, whereas plasma antioxidant activities and levels were significantly lower in RM patients, those of whole blood and placental tissue were much more significantly lower when compared with HP women (P<0.001). Concurrent with these findings there were consistent increases of equal statistical significance and magnitude in the levels of all investigated oxidants assayed in all samples when compared in between subjects of the study as indicated above. Data thus illustrated a distinct shift in favor of oxidative reactions and reactive oxygen species (ROS) generation, and very significant decreases in the GSH/GSSG ratios in whole blood and placental tissue of RM patients when compared to HP and NP women (P<0.001). The above noted oxidative stress could have been a major causative factor of recurrent miscarriage.

Pleiotropic roles of melatonin in endometriosis, recurrent spontaneous abortion, and polycystic ovary syndrome

“…79 The impaired placental development or degeneration of syncytiotrophoblast in early pregnancy may be caused by oxidative stress that leads to RSA. 81 Although reports suggest that the use of antioxidant supplements is beneficial in vitro fertilization, 82 studies related to their use in preventing miscarriage are very rare. A recent study has also found the enhanced ROS in blood and placental tissue of RSA patients.…”

“…A recent study has also found the enhanced ROS in blood and placental tissue of RSA patients. 81 Although reports suggest that the use of antioxidant supplements is beneficial in vitro fertilization, 82 studies related to their use in preventing miscarriage are very rare. Concerning the current research results, we speculate strongly that supplementary antioxidant therapy including Se, Zn, Cu, Mn, ascorbate, GSH, and αtocopherol, as well as melatonin, may be beneficial to such patients during pre-conception and the first-trimester post-conception.…”

The effect of Echis coloratus venom on biochemical and molecular markers of the antioxidant capacity in human fibroblasts

“…[33] The assay mixture contained sodium phosphate buffer (150 µl, pH 7.4), 8 mM EDTA (50 µl), DTNB solution (50 µl) and 2 mM NADPH (100 µl). GSH standards (20–80 µM) and fibroblast sonicates (50 µl) were then added and the tubes incubated at 37°C for 3 min.…”

Mutations in the zebrafishhmgcs1gene reveal a novel function for isoprenoids during red blood cell development

“…We first detected the number of We next quantified the decrease in circulating RBCs in homozygous mutants and their siblings. We performed a quantitative measure of total hemoglobin content using a colorimetric assay in which endogenous hemoglobin can be measured quantitatively at a wavelength of 400nm 44 . In order to measure the levels of hemoglobin in siblings and Vu57 carriers, homozygous Vu57 larval were separated according phenotype at 3 dpf 34 and the total hemoglobin content of homozygous carriers was compared to the hemoglobin content of wildtype and heterozygous siblings at 96 hpf.…”