Biochemical Mechanisms of Fatty Liver and Bioactive Foods

Sharma, Rajeev

doi:10.1016/b978-0-12-397154-8.00181-0

Cited by 5 publications

(4 citation statements)

References 116 publications

(105 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the last ten years, a correlation between an elevated lipid profile and liver enzymes has been demonstrated [ 30 , 31 ]. Therefore, in this work, we also investigated the effect of spirulina on the two major liver enzymes (i.e., ALT and AST), which are considered indicators of fatty liver infiltration due to lipid status disorders [ 32 ]. In agreement with the literature [ 33 ], the animals fed a high-fat diet showed higher AST and ALT levels compared to the control group ( Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…Increased faecal excretion of bile acids (e.g., using resins that sequester bile acids) stimulates ex novo synthesis of the same at the hepatic level, which determines the consumption of cholesterol withdrawn from the liver and plasma of the depot [ 41 , 42 ]. To meet the increased demand, the liver increases the expression of its receptors for LDL (bad cholesterol), which lowers total and LDL cholesterol levels [ 32 , 33 ]. To determine the effect of spirulina on the enterohepatic circulation of bile acids, in this work the content of the main primary and secondary bile acids (i.e., βMCA, CA, hyocholic acid, hyodeoxycholic acid, deoxycholic acid and lithocholic acid) in faeces was determined.…”

Section: Resultsmentioning

confidence: 99%

“…Our experiments showed that the levels of βMCA, CA, hyocholic acid, hyodeoxycholic acid, deoxycholic acid and lithocholic acids were significantly increased in the faeces of animals fed atherogenic diets compared to the control group (Table 3 In the last ten years, a correlation between an elevated lipid profile and liver enzymes has been demonstrated [30,31]. Therefore, in this work, we also investigated the effect of spirulina on the two major liver enzymes (i.e., ALT and AST), which are considered indicators of fatty liver infiltration due to lipid status disorders [32]. In agreement with the literature [33], the animals fed a high-fat diet showed higher AST and ALT levels compared to the control group (Figure 3).…”

Section: Chemical Composition Cholesterol and Bile Acid Content Of Fa...mentioning

confidence: 98%

See 2 more Smart Citations

Role of Arthrospira Platensis in Preventing and Treating High-Fat Diet-Induced Hypercholesterolemia in Adult Rats

Cacciola,

De Cicco,

Milanović

et al. 2024

Nutrients

View full text Add to dashboard Cite

Hyperlipidaemia is a recognised risk factor for cardiovascular disease. In this study, the antihyperlipidaemic properties of spirulina (Arthrospira platensis, strain S2 from Serbia) were tested in adult Wistar rats before and after induction of hypercholesterolaemia by a high-fat diet (HFD) to compare the preventive with the curative effect. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), alanine transaminase (ALT) and aspartate transaminase (AST) levels were measured in the blood samples. The chemical composition (lipids, proteins and cholesterol) and the content of bile acids in the faeces of the animals were also analysed. Feeding rats with an atherogenic diet for 10 weeks led to the successful development of hyperlipidaemia, as serum TC and LDL-C levels as well as lipids, cholesterol and bile acids in the animals’ faeces were significantly increased. Pre- and post-treatment with spirulina led to a reduction in serum LDL, TC and ALT levels. Administration of spirulina resulted in both a significant increase in primary bile acids excretion and a decrease in bile acids metabolism, with pre-treatment being more effective than post-treatment in some cases. These results suggest that increased excretion of bile acids as well as an effect on the gut microbiota may be the mechanism responsible for the anti-hyperlipidaemic activity of the tested spirulina strain.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Chemical Composition Cholesterol and Bile Acid Content Of Fa...mentioning

confidence: 98%

See 1 more Smart Citation

Role of Arthrospira Platensis in Preventing and Treating High-Fat Diet-Induced Hypercholesterolemia in Adult Rats

Cacciola,

De Cicco,

Milanović

et al. 2024

Nutrients

View full text Add to dashboard Cite

show abstract

“…Environmental pH is one the factors that affects protein structure (Monahan et al 1995). The appropriate protein structure is crucial for the protein to function (Sharma 2013). Thus, pH optimization was crucial for SR74 α-amylase production in the BMMY medium.…”

Section: Effect Of Different Media On Recombinant α-Amylase Productionmentioning

confidence: 99%

Molecular expression of a recombinant thermostable bacterial amylase from Geobacillus stearothermophilus SR74 using methanol-free Meyerozyma guilliermondii strain SO yeast system

Nasir

Leow

Oslan

et al. 2020

BioRes

View full text Add to dashboard Cite

α-Amylase, which was isolated from Geobacillus stearothermophilus SR74, has shown its potential to be used in industrial applications. However, its expression in the Pichia pastoris expression system with the alcohol oxidase 1 promoter (PAOX1) requires high methanol consumption and is time-consuming. This study aimed to express SR74 α-amylase in an alternative yeast system, using Meyerozyma guilliermondii strain SO, which was isolated from a spoiled orange (SO) under the regulation of a formaldehyde dehydrogenase promoter (PFLD). Qualitative screening showed that strain SO possessed a native amylase grown on YPD-starch plate at 30 °C. The recombinant SR74 α-amylase was further quantified and validated using the Western blot test. It was confirmed that SR74 α-amylase was expressed by strain SO extracellularly with a size of 59 kDa. Optimization in a shake flask showed that the recombinant SR74 α-amylase, which was regulated by PFLD, was successfully produced (26 U/mL) without any external inducer in the YPT medium after 24 h of cultivation. In conclusion, strain SO was able to produce SR74 amylase without methanol in one-fifth the fermentation time of P. pastoris. Further optimization of the expression may be done to improve the yield, as this methanol-free host is still underexplored.

show abstract

A Systematic Review of Proteomics in Obesity: Unpacking the Molecular Puzzle

Rodriguez-Muñoz,

Motahari-Rad,

Martin-Chaves

et al. 2024

Curr Obes Rep

View full text Add to dashboard Cite

Purpose of Review The present study aims to review the existing literature to identify pathophysiological proteins in obesity by conducting a systematic review of proteomics studies. Proteomics may reveal the mechanisms of obesity development and clarify the links between obesity and related diseases, improving our comprehension of obesity and its clinical implications. Recent Findings Most of the molecular events implicated in obesity development remain incomplete. Proteomics stands as a powerful tool for elucidating the intricate interactions among proteins in the context of obesity. This methodology has the potential to identify proteins involved in pathological processes and to evaluate changes in protein abundance during obesity development, contributing to the identification of early disease predisposition, monitoring the effectiveness of interventions and improving disease management overall. Despite many non-targeted proteomic studies exploring obesity, a comprehensive and up-to-date systematic review of the molecular events implicated in obesity development is lacking. The lack of such a review presents a significant challenge for researchers trying to interpret the existing literature. Summary This systematic review was conducted following the PRISMA guidelines and included sixteen human proteomic studies, each of which delineated proteins exhibiting significant alterations in obesity. A total of 41 proteins were reported to be altered in obesity by at least two or more studies. These proteins were involved in metabolic pathways, oxidative stress responses, inflammatory processes, protein folding, coagulation, as well as structure/cytoskeleton. Many of the identified proteomic biomarkers of obesity have also been reported to be dysregulated in obesity-related disease. Among them, seven proteins, which belong to metabolic pathways (aldehyde dehydrogenase and apolipoprotein A1), the chaperone family (albumin, heat shock protein beta 1, protein disulfide-isomerase A3) and oxidative stress and inflammation proteins (catalase and complement C3), could potentially serve as biomarkers for the progression of obesity and the development of comorbidities, contributing to personalized medicine in the field of obesity. Our systematic review in proteomics represents a substantial step forward in unravelling the complexities of protein alterations associated with obesity. It provides valuable insights into the pathophysiological mechanisms underlying obesity, thereby opening avenues for the discovery of potential biomarkers and the development of personalized medicine in obesity

show abstract

Biochemical Mechanisms of Fatty Liver and Bioactive Foods

Cited by 5 publications

References 116 publications

Role of Arthrospira Platensis in Preventing and Treating High-Fat Diet-Induced Hypercholesterolemia in Adult Rats

Role of Arthrospira Platensis in Preventing and Treating High-Fat Diet-Induced Hypercholesterolemia in Adult Rats

Molecular expression of a recombinant thermostable bacterial amylase from Geobacillus stearothermophilus SR74 using methanol-free Meyerozyma guilliermondii strain SO yeast system

A Systematic Review of Proteomics in Obesity: Unpacking the Molecular Puzzle

Contact Info

Product

Resources

About