Biochemical observations on beagle dog semen

Rw, James; Heywood, R.; Ae, Street

doi:10.1136/vr.104.21.480

Cited by 12 publications

(7 citation statements)

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“…Comparison of our data with the previously published results is complicated because several authors analysed seminal plasma of mixed ejaculate fractions (De Souza, Martins, & Lopes, ) or did not mention which fractions the seminal plasma was obtained from (James, Heywood, & Street, ; Schäfer‐Somi, Blickensdörfer, & Schwendenwein, ). Additionally, preparation of seminal plasma differed considerably between studies in term of centrifugation speed (range: 700 g–4000 g) and duration (range: 15–30 min).…”

Section: Discussionmentioning

confidence: 66%

Concentrations of minerals in the canine prostatic fluid

Umbach¹,

Failing

Goericke-Pesch

et al. 2019

Reprod Domestic Animals

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Current knowledge about the composition of the prostate fluid in healthy male dogs is limited and restricted to small case numbers. Furthermore, published data often vary significantly regarding sample processing and analytical methods. Therefore, we aimed to provide data on the composition of electrolytes and minerals in the canine prostatic fluid in a larger population (n = 30 dogs/samples) and to compare these results with the existing literature. Concentrations of sodium, potassium and copper analysed in our population were most consistent with those in the literature. Different to this, concentrations of total calcium, magnesium, zinc and inorganic phosphate varied. Whereas magnesium, zinc and inorganic phosphate seemed to depend on the analysis method, total calcium concentrations differed if centrifugation was performed or not. Our results clearly indicate a need for standardization of methods for analysis of seminal plasma components. K E Y W O R D S dog, minerals, prostatic fluid, seminal plasma | 1065 UMBACH et Al.

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Section: Discussionmentioning

confidence: 66%

Concentrations of minerals in the canine prostatic fluid

Umbach¹,

Failing

Goericke-Pesch

et al. 2019

Reprod Domestic Animals

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“…Also in line with these findings, Johnston (1991) reported that normospermic dogs have concentrations of AP in seminal plasma that ranged from 5000 to 40 000 U/L. ; whereas James et al (1979) reported AP concentrations that ranged from 5500 to 14 000 U/L. AP concentrations in either semen fractions or in seminal plasma (after centrifugation) are a useful test to differentiate between the collection of an incomplete ejaculate (little or no second fraction collected), obstructive azoospermia (obstruction of the vasa deferentia or epididymides) and azoospermia as a result of gonadal dysfunction (testicular hypoplasia and degeneration) or to unilateral or bilateral aplasia of the epididymides (Johnston 1991, Olson et al 1992, Meyers‐Wallen 1995).…”

Section: Discussionmentioning

confidence: 54%

Unilateral and Bilateral Vasectomy in the Dog: Alkaline Phosphatase as an Indicator of Tubular Patency

Stornelli¹,

Arauz²,

Baschard

et al. 2003

Reprod Domestic Animals

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The objective of this research was to use a model of unilateral and bilateral occlusion of the ductus deferens in the dog to study the use of alkaline phosphatase (AP) as an indicator of tubular patency. Seven healthy cross bred dogs weighing 10-15 kg BW with normal spermiogram and AP concentrations in semen were used. From each dog, three semen samples were obtained before (intact) and after right (unilateral) and left (bilateral) vasectomy. The AP concentrations were measured in duplicates by a colorimetric method in each of the three fractions (first, second (sperm-rich), third) of each ejaculate. In addition, a macroscopic and microscopic evaluation of each ejaculate was carried out to assure its quality. Data were analysed by least squares analysis of variance using SAS(R). In intact and unilateral vasectomized dogs, 96.6% of AP measured in semen corresponded to the second sperm-rich fraction whereas 1.53 and 1.83% corresponded to the first and third fractions respectively. Total AP concentrations (first and second and third fraction) in vasectomized dogs were lower than in intact animals (19.857 vs 2284.431 +/- 4.347 UAL; p < 0.001). AP concentrations were much lower in bilateral than in unilateral vasectomized dogs (142 vs 39.572 +/- 4.347 UL, p < 0.001). In summary, AP concentrations in semen can be used as an early indicator of unilateral or bilateral lack of patency of the epididymal and deferent ducts in the dog.

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“…The normal semen quality values in adult Beagle dogs are reported to be: total semen volume, 7-12 ml; total number of sperm, 300-500 × 10 6 ; sperm motility, 80-90%; and percentages of abnormal sperm and immature sperm, 5-12% and 1-2%, respectively [3,17,20,22,38,40]. Normal values of pH, osmotic pressure, and Na + ·K + concentrations in the second semen fraction of dogs are reported to be of 6.7-6.8, 280-300 mOs/kg, 192 m M/l and 12.4 m M/l, respectively [2,6,22,43].…”

Section: Discussionmentioning

confidence: 99%

“…The body weight of the Beagle dogs and the mongrel dogs ranged from 12 kg to 15 kg, and the Shetland sheep dog weighed 20 kg. All of the dogs were diagnosed as having both asthenozoospermia and teratozoospermia, based on a comparison of their semen quality (Table 1) and with other reports on normal semen quality in dogs [17,20,38]. The dogs were housed in pens with runs under natural lighting, and water was available ad libitum.…”

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confidence: 99%

Changes in Semen Quality and In Vitro Sperm Capacitation during Various Frequencies of Semen Collection in Dogs with Both Asthenozoospermia and Teratozoospermia.

1998

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ABSTRACT. Eight male dogs with both asthenozoospermia and teratozoospermia were used in this study. In experiment 1, semen was collected 10 times at intervals of 48 hr, 24 hr and 12 hr in 4 of the 8 dogs, and the semen quality was evaluated. In experiment 2, semen was collected 5 times at 24-hr intervals in the other 4 dogs. The spermatozoa collected on day 1 and day 5 were incubated for 4-6 hr in Canine Capacitation Medium, and the percentages of hyperactivated sperm (%HA), acrosome-reacted sperm (%AR), and the zona pellucida-binding sperm count (ZP sperm count) were assessed. The results of experiment 1 showed that the percentage of motile sperm increased and the percentage of abnormal sperm decreased markedly as the intervals between semen collections became shorter. When semen was collected at 12-hr intervals, the percentage of motile sperm increased from about 65%, the value before frequent collection was started, to about 80%, and the percentage of sperm with abnormal tails decreased from 30% to15%. In experiment 2, the percentages of HA and AR, and the ZP sperm count in specimens collected on day 5 were higher than those in specimens collected on day 1, and the differences in % HA and in ZP sperm count were significant (P<0.05). The results demonstrated that sperm motility, abnormality, and potential fertility in dogs with asthenozoospermia and teratozoospermia can be temporarily improved by frequent semen collection. -KEY WORDS: asthenozoospermia, canine, frequent semen collection, teratozoospermia.J. Vet. Med. Sci. 60 (5): [607][608][609][610][611][612][613][614] 1998 years, were used. The body weight of the Beagle dogs and the mongrel dogs ranged from 12 kg to 15 kg, and the Shetland sheep dog weighed 20 kg. All of the dogs were diagnosed as having both asthenozoospermia and teratozoospermia, based on a comparison of their semen quality (Table 1) and with other reports on normal semen quality in dogs [17,20,38]. The dogs were housed in pens with runs under natural lighting, and water was available ad libitum. Semen evaluation: The male dogs were trained so that their semen could be collected by digital manipulation without an estrous bitch. Semen samples were examined for total semen volume, total number of sperm, and percentages of actively motile sperm, viable sperm, and morphologically abnormal and immature sperm using methods previously described [23]. Briefly, the concentration of sperm in the semen was determined by hematocytometer counts. The percentages of motile sperm and viable sperm were estimated by counting actively motile sperm on a warmed slide glass and by the eosin-nigrosin staining method, respectively. Sperm morphology was examined by the aniline blue and eosin staining method [5]. Spermatozoa with a cytoplasmic droplet attached to the midpiece were counted as immature sperm [39]. It has been known that the ejaculate in dogs can be divided into 3 fractions [15]. In the present study, the pH and osmolarity (Vapor Pressure Osmometer; Model 5100, Wescor, Inc., U.S.A.) of the second s...

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Biochemical observations on beagle dog semen

Cited by 12 publications

References 0 publications

Concentrations of minerals in the canine prostatic fluid

Concentrations of minerals in the canine prostatic fluid

Unilateral and Bilateral Vasectomy in the Dog: Alkaline Phosphatase as an Indicator of Tubular Patency

Changes in Semen Quality and In Vitro Sperm Capacitation during Various Frequencies of Semen Collection in Dogs with Both Asthenozoospermia and Teratozoospermia.

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