Biochemical Origin of Raman-Based Diagnostics of Huanglongbing in Grapefruit Trees

Dou, Tianyi; Sanchez, Lee; Irigoyen, Sonia; Goff, Nicolas K.; Niraula, Prakash M.; Mandadi, Kranthi K.; Kurouski, Dmitry

doi:10.3389/fpls.2021.680991

Cited by 25 publications

(16 citation statements)

References 49 publications

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“…In particular, a reduced concentration of carotenoids in grapevine suggests a functional link to either a modulation of transcripts involved in carotenoid metabolism or to their degradation and fragmentation or conversion to apocarotenoids, i.e., signaling molecules produced in response to stress. A decrease in carotenoid concentration has been frequently reported when analyzing by Raman spectroscopy plants infected by pathogens ( Dou et al, 2021 ; Farber et al, 2021 ; Vallejo-Pérez et al, 2021 ) or subjected to abiotic stresses ( Altangerel et al, 2017 ; Sng et al, 2020 ), confirming the role of this class of molecules in plant stress responses.…”

Section: Resultsmentioning

confidence: 55%

“…A near infrared laser wavelength was used to limit the undesired fluorescence effect disturbing Raman signals. Other research paper dealing with Raman measurements on plant tissues report the alternative use of 785 nm ( Dou et al, 2021 ), 830 nm ( Farber et al, 2019b ; Sanchez et al, 2020 ; Payne et al, 2022 ), or 1,064 nm ( Yeturu et al, 2016 ; Farber and Kurouski, 2018 ; Skoczowski et al, 2022 ) laser wavelengths to minimize fluorescence interference and increase signal-to-noise ratio. At the same time, a relatively low laser power and low magnification objective were adopted to avoid thermal stress of the tissue and to collect information from a relatively large area (spot size >2 μm).…”

Section: Resultsmentioning

confidence: 99%

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Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses

Mandrile

D’Errico²,

Nuzzo³

et al. 2022

Front. Plant Sci.

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Grapevine is one of the most cultivated fruit plant among economically relevant species in the world. It is vegetatively propagated and can be attacked by more than 80 viruses with possible detrimental effects on crop yield and wine quality. Preventive measures relying on extensive and robust diagnosis are fundamental to guarantee the use of virus-free grapevine plants and to manage its diseases. New phenotyping techniques for non-invasive identification of biochemical changes occurring during virus infection can be used for rapid diagnostic purposes. Here, we have investigated the potential of Raman spectroscopy (RS) to identify the presence of two different viruses, grapevine fan leaf virus (GFLV) and grapevine rupestris stem pitting-associated virus (GRSPaV) in Vitis vinifera cv. Chardonnay. We showed that RS can discriminate healthy plants from those infected by each of the two viruses, even in the absence of visible symptoms, with accuracy up to 100% and 80% for GFLV and GRSPaV, respectively. Chemometric analyses of the Raman spectra followed by chemical measurements showed that RS could probe a decrease in the carotenoid content in infected leaves, more profoundly altered by GFLV infection. Transcriptional analysis of genes involved in the carotenoid pathway confirmed that this biosynthetic process is altered during infection. These results indicate that RS is a cutting-edge alternative for a real-time dynamic monitoring of pathogens in grapevine plants and can be useful for studying the metabolic changes ensuing from plant stresses.

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Section: Resultsmentioning

confidence: 55%

Section: Resultsmentioning

confidence: 99%

Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses

Mandrile

D’Errico²,

Nuzzo³

et al. 2022

Front. Plant Sci.

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show abstract

“…For example, the peak at 1657 cm -1 could represent vibrations from an amide bond, characteristic of all proteins, or an alkene group in unsaturated fatty acids ( Khan et al., 2016 ; Farber et al., 2020 ; Farber et al., 2021 ). To accurately identify compounds and their functional groups, high performance liquid chromatography (HPLC) could potentially be applied ( Dou et al., 2021 ; Higgins et al., 2022 ). Previous utilization of HPLC coupled to mass spectrometry has successfully identified changes in the metabolic profile of the blood from patients diagnosed with early-stage LD ( Molins et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Testing Raman spectroscopy as a diagnostic approach for Lyme disease patients

Goff

Dou²,

Higgins³

et al. 2022

Front. Cell. Infect. Microbiol.

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Lyme disease (LD), the leading tick-borne disease in the Northern hemisphere, is caused by spirochetes of several genospecies of the Borreliella burgdorferi sensu lato complex. LD is a multi-systemic and highly debilitating illness that is notoriously challenging to diagnose. The main drawbacks of the two-tiered serology, the only approved diagnostic test in the United States, include poor sensitivity, background seropositivity, and cross-reactivity. Recently, Raman spectroscopy (RS) was examined for its LD diagnostic utility by our earlier proof-of-concept study. The previous investigation analyzed the blood from mice that were infected with 297 and B31 strains of Borreliella burgdorferi sensu stricto (s.s.). The selected strains represented two out of the three major clades of B. burgdorferi s.s. isolates found in the United States. The obtained results were encouraging and prompted us to further investigate the RS diagnostic capacity for LD in this study. The present investigation has analyzed blood of mice infected with European genospecies, Borreliella afzelii or Borreliella garinii, or B. burgdorferi N40, a strain of the third major class of B. burgdorferi s.s. in the United States. Moreover, 90 human serum samples that originated from LD-confirmed, LD-negative, and LD-probable human patients were also analyzed by RS. The overall results demonstrated that blood samples from Borreliella-infected mice were identified with 96% accuracy, 94% sensitivity, and 100% specificity. Furthermore, human blood samples were analyzed with 88% accuracy, 85% sensitivity, and 90% specificity. Together, the current data indicate that RS should be further explored as a potential diagnostic test for LD patients.

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“…A reduced carotene content has a strong physiological correlation with plant defence mechanisms. Dou et al (2021) used Raman spectroscopy (RS) to analyse the spectral signature of citrus leaves and detected reduced concentration of lutein. This information can be used to determine the potential of biotic and abiotic stress in plant molecular mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Nondestructive determination of carotenoids in kiwifruit leaves infected with Pseudomonas syringae pv. actinidiae by surface‐enhanced Raman spectroscopy combined with chemical imaging

et al. 2023

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Surface-enhanced Raman spectroscopy (SERS), a noninvasive and nondestructive technique, was used to distinguish healthy and cankered kiwifruit leaves. Silver nanoparticles with iodide and calcium ions (Ag@ICNPs) were synthesized as SERS substrates and significantly enhanced the characteristic signal peaks of carotenoids.Due to its simple operation and high sensitivity, the detection process is straightforward and efficient. Using a 532 nm laser excitation source, spectra were collected from the leaf surface over a wavelength range of 400-2000 cm −1 , and the characteristic carotenoid peak at 1520 cm −1 was selected for integral imaging. Detection of changes in carotenoid spectral characteristics on the surface of kiwifruit leaves could be used as an early warning of kiwifruit canker. Raman spectroscopy and high-performance liquid chromatography confirmed the existence of two main carotenoids, lutein and β-carotene, and their intensity changed significantly following infection, especially in the later stages of infection. The results of principal component analysis and support vector machine (SVM) analysis of preprocessed spectra showed that samples of healthy, early disease and advanced disease leaves clustered separately, and the accuracy of SVM classification was up to 98%. The results of chemical imaging showed that the average carotenoid signal decreased with extended incubation time and there were significant differences among healthy, early disease and advanced disease stages. Therefore, SERS can be used as a general tool for in situ nondestructive determination of surface carotenoid changes in kiwifruit leaves during disease, and thereby effectively monitor the nutritional quality of kiwifruit before harvest.

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Biochemical Origin of Raman-Based Diagnostics of Huanglongbing in Grapefruit Trees

Cited by 25 publications

References 49 publications

Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses

Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses

Testing Raman spectroscopy as a diagnostic approach for Lyme disease patients

Nondestructive determination of carotenoids in kiwifruit leaves infected with Pseudomonas syringae pv. actinidiae by surface‐enhanced Raman spectroscopy combined with chemical imaging

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