Biochemical Profile, Liver and Kidney Selenium (Se) Status during Acanthamoebiasis in a Mouse Model

Łanocha-Arendarczyk, Natalia; Baranowska-Bosiacka, Irena; Kot, Karolina; Pilarczyk, Bogumiła; Tomza–Marciniak, Agnieszka; Kabat‐Koperska, Joanna; Kosik-Bogacka, Danuta

doi:10.3409/fb_66-1.04

Cited by 17 publications

(21 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the re-isolation of Acanthamoeba spp. from the mouse kidneys, the kidney pro le performed in mouse serum indicated normal renal function [7]. Therefore, based on previous studies, the a nity of Acanthamoeba spp.…”

Section: Discussionmentioning

confidence: 94%

“…In our earlier study concerning hematological and biochemical pro les in the blood of experimentally Acanthamoeba spp. infected mice, we found only higher level of lymphocytes, monocytes, thrombocytes and aspartate aminotransferase [7,8], despite the re-isolation of these amoebae from the brain, eyeball, lungs, kidneys, spleen and heart of the mice [7-11, unpublished data]. In another study concerning histopathological changes in the kidneys and heart of mice infected with Acanthamoeba spp., we observed some histopathological changes, including relaxation of muscle bres, elevated proliferation of cellular nuclei in the proximal/distal tubule epithelium and areas with less acidic cytoplasm [unpublished data].…”

Section: Introductionmentioning

confidence: 83%

See 1 more Smart Citation

The Expression of TLR2 and TLR4 in the Kidneys and Heart of Mice Infected with Acanthamoeba Spp.

Kot

Kosik-Bogacka

Wojtkowiak-Giera

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Acanthamoeba spp. are cosmopolitan protozoa that cause infections in the brain, as well as extracerebral infections in the cornea, lungs and skin. Little is known about the mechanisms of the immunological response to these parasites in organs which are not their main biotope. Therefore, the purpose of this study was to determine the expression of TLR2 and TLR4 in the kidneys and heart of Acanthamoeba spp. infected mice, with respect to the host’s immunological status. Results: In the kidneys, we observed a higher expression of TLR2 in immunosuppressed mice at 24 days post Acanthamoeba spp. infection (dpi) compared to the uninfected mice. There were no statistically significant differences in TLR4 expression in the kidneys between the immunocompetent and immunosuppressed mice, both of infected and uninfected mice. In the heart, we observed a difference in TLR2 expression in immunocompetent mice at 24 dpi compared to immunocompetent mice at 8 dpi. The immunocompetent Acanthamoeba spp. infected mice had higher TLR4 expression at 8 dpi compared to the immunocompetent uninfected mice. Conclusions: Our results indicate that TLR2 is involved in response to Acanthamoeba spp. infection in the kidneys, whereas in the heart, both studied TLRs are involved.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 83%

The Expression of TLR2 and TLR4 in the Kidneys and Heart of Mice Infected with Acanthamoeba Spp.

Kot

Kosik-Bogacka

Wojtkowiak-Giera

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…In our experimental model, despite the re-isolation of Acanthamoeba spp. from the mouse kidneys, the kidney profile performed in mouse serum indicated normal renal function [ 7 ]. In the study concerning histopathological changes in the kidneys of mice infected with Acanthamoeba spp.…”

Section: Discussionmentioning

confidence: 99%

“…Disseminated acanthamoebiasis is predominantly confirmed post-mortem by histopathological examination of the organs and/or re-isolation of amoebae from tissue fragments because biochemical and hematological tests are difficult to interpret; blood parameters may be elevated, lower or not deviating from the norm [ 5 , 6 ]. In our earlier study concerning hematological and biochemical profiles in the blood of experimentally Acanthamoeba spp.-infected mice, we found only higher level of lymphocytes, monocytes, thrombocytes and aspartate aminotransferase [ 7 , 8 ], despite the re-isolation of these amoebae from the brain, eyeball, lungs, kidneys, and spleen of the mice [ 7 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

The expression of TLR2 and TLR4 in the kidneys and heart of mice infected with Acanthamoeba spp.

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background Acanthamoeba spp. are cosmopolitan protozoans that cause infections in the brain, as well as extracerebral infections in the cornea, lungs and skin. Little is known about the mechanisms of the immunological response to these parasites in organs which are not their main biotope. Therefore, the purpose of this study was to determine the expression of TLR2 and TLR4 in the kidneys and heart of Acanthamoeba spp.-infected mice, with respect to the host’s immunological status. Methods The mice were grouped into four groups: immunocompetent control mice; immunosuppressed control mice; immunocompetent Acanthamoeba spp.-infected mice; and immunosuppressed Acanthamoeba spp. infected mice. In the study, we used the amoebae T16 genotype which was isolated from a patient. The TLRs expressions in the kidneys and heart of mice were assessed by quantitative real-time polymerase chain reaction. Moreover, we visualized TLR2 and TLR4 proteins in the organs by immunohistochemical staining. Results In the kidneys, we observed a higher TLR2 expression in immunosuppressed mice at 24 days post-Acanthamoeba spp. infection (dpi) compared to the uninfected mice. There were no statistically significant differences in TLR4 expression in the kidneys between the immunocompetent and immunosuppressed mice, both of infected and uninfected mice. In the heart, we observed a difference in TLR2 expression in immunocompetent mice at 24 dpi compared to immunocompetent mice at 8 dpi. The immunocompetent Acanthamoeba spp.-infected mice had higher TLR4 expression at 8 dpi compared to the immunocompetent uninfected mice. Conclusions Our results indicate that TLR2 is involved in response to Acanthamoeba spp. infection in the kidneys, whereas in the heart, both studied TLRs are involved.

show abstract

“…Our experimental animal model has been described in our previous research [33,34]. Adult male, Balb/c mice (~23 g, 6-10 months, Center of Experimental Medicine, Medical University in Białystok, Poland) were housed individually on a 12h:12h light/dark cycle under controlled temperature with free access to food and water.…”

Section: Animal Modelmentioning

confidence: 99%

Expression of Toll-Like Receptors (TLR2 and TLR4) in the Eyes of Mice Intranasally Infected with <em>Acanthamoeba</em> sp.

Kot¹,

Kosik-Bogacka²,

Łanocha-Arendarczyk³

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Toll-like receptors (TLRs) play a key role in the innate immune response to numerous pathogens, including Acanthamoeba sp. The aim of this study was to determine the expression of TLR2 and TLR4 in the eyes of mice following intranasal infection with Acanthamoeba sp. Amoebae used in this study were isolated from the bronchial aspirate of a patient with acute myeloid leukemia (AML) and atypical symptoms of pneumonia. We found statistically significant differences in the expression of TLR2 and TLR4 in the eyes of immunocompetent mice at 8, 16, and 24 days post Acanthamoeba sp. infection (dpi) compared to control. Immunosuppressed mice showed significant differences in the expression of TLR2 at 16 and 24 dpi compared to uninfected animals. Our results indicate that TLR2 and TLR4 are upregulated in the eyes of mice in response to Acanthamoeba sp. We suggest that it is possible for trophozoites to migrate through the optic nerve from the brain to the eyes.

show abstract

Biochemical Profile, Liver and Kidney Selenium (Se) Status during Acanthamoebiasis in a Mouse Model

Cited by 17 publications

References 0 publications

The Expression of TLR2 and TLR4 in the Kidneys and Heart of Mice Infected with Acanthamoeba Spp.

The Expression of TLR2 and TLR4 in the Kidneys and Heart of Mice Infected with Acanthamoeba Spp.

The expression of TLR2 and TLR4 in the kidneys and heart of mice infected with Acanthamoeba spp.

Expression of Toll-Like Receptors (TLR2 and TLR4) in the Eyes of Mice Intranasally Infected with <em>Acanthamoeba</em> sp.

Contact Info

Product

Resources

About