Biochemical profile of the Pêga donkey (Equus asinus) breed: influence of age and sex

Girardi, Annita Morais; Marques, Luiz Carlos; Toledo, Carmen Zilda Pereira de; Barbosa, José Carlos; Maldonado, Walter; Jorge, Renata Lemos Nagib; Nogueira, Cláudia Aparecida da Silva

doi:10.1007/s00580-013-1718-4

Cited by 27 publications

(64 citation statements)

References 14 publications

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“…The level of TP was greater for older animals, which is similar to other studies, where an increase level of total protein with age was described (Zinkl et al 1990;Folch et al 1997;Girardi et al 2013), possibly due to the increasing amount of globulins.…”

Section: Discussionsupporting

confidence: 90%

“…This can be due to the difference in muscle mass (due to different exercise) between the studied groups. However, no effect of age on CK was found in other studies (French and Patrick 1995;Girardi et al 2013).…”

Section: Discussionmentioning

confidence: 59%

“…The influence of the donkey's age and sex has also already been reported (Folch et al 1997;Jordana et al 1998;Mori et al 2003Mori et al , 2004Girardi et al 2013Girardi et al , 2014. On the other hand, there is a study which investigated about 4000 donkeys without proof that biochemical variables are affected by the age and sex (French and Patrick 1995).…”

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confidence: 94%

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Biochemical and haematological profile of donkeys in the Czech and Slovak Republics: influence of age and sex

et al. 2017

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The aim of this study was to evaluate the importance of taking into account the age and sex of the donkeys while assessing individual haematological and biochemical variables. Material used for the study were blood samples collected from 112 clinically healthy donkeys (37 males and 75 females) aged from 05 to 30 years old. Selected haematological and biochemical variables were examined and the influence of sex and age was analyzed by multivariate analysis. Significant differences were found in some of biochemical and haematological variables between groups. The results of this study can be useful for appropriate clinical interpretation of laboratory results in donkeys.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 59%

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Biochemical and haematological profile of donkeys in the Czech and Slovak Republics: influence of age and sex

et al. 2017

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“…As a reference, a marker solution (SigmaMarker wide range, Sigma-Aldrich, St Louis, USA) with molecular weights ranging from 6.5 to 200 kDa, was used. The serum protein fraction concentrations (mg/dl) were determined by multiplying the percentage of each fraction by the total protein concentrations, obtained through a semi-automatic spectrophotometer analyser (LabQuest, Labtest Diagnostica S.A., Lagoa Santa, Brazil) using biuret the method as previously described (Girardi et al 2014). These analyses were made at the Research Laboratory of the Department of Veterinary Clinic and Surgery, School of Agrarian and Veterinary Sciences, Sao Paulo State University, Jaboticabal, Brazil.…”

Section: Methodsmentioning

confidence: 99%

“…Currently, the Brazilian Association of Pega Donkey Breeders (ABCJ Pega) has approximately 2000 members and about 20 000 mules and donkeys registered (Canisso and McDonnell 2010). However, only a limited amount of research has been performed on the haematological and serum biochemical variables of this breed (Campos et al 1968;Girardi et al 2014;Girardi et al 2015;Girardi et al 2016).…”

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confidence: 99%

The effect of age and sex on serum proteins in the Pega donkey (Equus asinus)

Girardi¹,

Toledo²,

Silva³

et al. 2017

Vet. Med. - Czech

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ABSTRACT:In this study sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to evaluate the influence of age and sex on serum proteins in 110 Pega donkeys, 79 females and 31 males, classified into three age groups (under one year -eight animals; one to three years -33 animals and over three years old -69 animals). SDS-PAGE allowed identification of 10 serum proteins, some with unknown functions. No age-related differences were observed (P > 0.05) for haptoglobin, α 1 -acid glycoprotein and 23 kDa molecular weight protein (MWP 23 ) and no sex-related differences (P > 0.05) for immunoglobulin A, albumin, MWP 23 and haptoglobin. With advancing age, immunoglobulin A levels decreased (P < 0.001), whereas immunoglobulin G levels increased (P < 0.001). The youngest donkeys showed the lowest albumin (P < 0.001) and 33 kDa molecular weight protein (MWP 33 ) (P < 0.05) means. The oldest group showed a higher transferrin mean (P < 0.05) than the youngest one. Animals between one and three years had the highest mean (P < 0.001) for 138 kDa molecular weight protein (MWP 138 ). Animals over three years showed higher ceruloplasmin levels (P < 0.05) than the one-to-three year old group. Males showed greater means of MWP 138 (P < 0.05), ceruloplasmin (P < 0.05), α 1 -acid glycoprotein (P < 0.001) and MWP 33 (P < 0.01). Females had higher transferrin (P < 0.001) and immunoglobulin G (P < 0.001) concentrations. Sex and age influenced the levels of the majority of proteins in the serum profile of Pega donkeys, including some acute phase proteins. Our results differ from those of previous studies in the effects of breed and environmental factors on some of the measured variables.

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Hematological variables of the Pêga donkey (Equus asinus) breed: influence of age and sex

et al. 2014

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Appropriate hematological reference intervals mitigate the risk of unnecessary investigations and failures on disease diagnosis. Species, breed, environment, handling, and physiologic stage could influence hematological variables. In this context, there is a lack of hematological reference intervals for Pêga donkeys, an important Brazilian asinine breed. This study aimed to establish hematological reference values for Pêga donkeys and determine the influence of age and sex on these variables. The complete blood count of samples collected from 110 animals, 79 females and 31 males was performed, maintained under field conditions. The animals under 1 year old (three females and five males) were monitored by collections on the day of birth, on 3rd, 7th, 15th day, and monthly until 12 months of life, while the other donkeys were sampled once. There were differences in several variables analyzed, confirming the requirement of different reference intervals according to age group and sex. Moreover, differences among the values obtained and those of previous studies show a breed influence on the hematological variables of donkeys. For Pêga breed donkeys, animal's age and sex influence most of the hematological variables. This study can be useful in the clinical routine and serve as basis to other scientific researches related to Pêga donkeys.

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Biochemical profile of the Pêga donkey (Equus asinus) breed: influence of age and sex

Cited by 27 publications

References 14 publications

Biochemical and haematological profile of donkeys in the Czech and Slovak Republics: influence of age and sex

Biochemical and haematological profile of donkeys in the Czech and Slovak Republics: influence of age and sex

The effect of age and sex on serum proteins in the Pega donkey (Equus asinus)

Hematological variables of the Pêga donkey (Equus asinus) breed: influence of age and sex

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