Biochemical Properties of Bovine Granulocytes

Gennaro, Renato; Schneider, Claudio; Nicola, G. de; Cian, Franca; Romeo, Domenico

doi:10.3181/00379727-157-40050

Cited by 48 publications

(26 citation statements)

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“…Secretory granules, as such, have not been described in bovine neutrophils; however, the accepted marker for this granule, alkaline phosphatase, exists in relatively high amounts in bovine neutrophil plasma membrane preparations [18,52]. Furthermore, the total alkaline phosphatase content of bovine neutrophils is 50-55% higher than the unstimulated cell surface alkaline phosphatase activity, indicating latent, potentially granular alkaline phosphatase.…”

Section: Discussionmentioning

confidence: 99%

“…For example, bovine neutrophils contain a unique large granule that is not present in human cells [17]. In addition, bovine neutrophils contain much lower concentrations of lysozyme as compared to human neutrophils [18]. However, one of the most unusual features of bovine neutrophils is their lack of response to N-formyl chemotactic peptides, reportedly due to the absence of detectable N-formyl peptide receptors [19,20].…”

Section: Introductionmentioning

confidence: 99%

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Platelet-activating factor induces a concentration-dependent spectrum of functional responses in bovine neutrophils

Swain

Bunger

Sipes

et al. 1998

Journal of Leukocyte Biology

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We characterized the dose response of bovine neutrophils to platelet-activating factor (PAF) with respect to the following functions: calcium flux and membrane potential changes, actin polymerization, degranulation, and the production and/or priming of the oxidative burst. PAF at very low concentrations (10 -10 and 10 -9 M) caused changes in intracellular calcium and membrane potential in bovine neutrophils, whereas moderate PAF concentrations (H10 -7 M) resulted in increased actin polymerization. Degranulation responses to PAF were more complex: low concentrations (10 -9 M) caused secretory granule degranulation, moderate doses (H10 -7 M) caused specific granule degranulation, whereas azurophil degranulation only occurred at high (10 -5 M) PAF concentrations. Treatment of bovine neutrophils with PAF at concentrations H10 -7 M also caused up-regulation of the adhesion molecules Mac-1 and L-selectin. PAF stimulation resulted in a very weak [compared to phorbol myristate acetate (PMA)] oxidative burst in bovine neutrophils, and only at high (10 -6 M) concentrations. Unlike human neutrophils, bovine neutrophils were poorly primed by PAF treatment. Only high concentrations of PAF (10 -5 M) caused an increased rate of PMA-stimulated superoxide production, although lower doses of PAF did reduce the lag time preceding the PMA-induced oxidative burst. The overall pattern that can be inferred is that lower concentrations of PAF promote neutrophil sensitivity and interaction by selective degranulation, up-regulation of adhesion molecules, and increased actin polymerization. In contrast, higher PAF concentrations can promote, albeit weakly, more direct bactericidal responses, such as the release of reactive oxygen species and granule enzymes. The ability of PAF to modulate a graded response in bovine neutrophils would allow the cell to respond proportionally to the severity of a stimulus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Platelet-activating factor induces a concentration-dependent spectrum of functional responses in bovine neutrophils

Swain

Bunger

Sipes

et al. 1998

Journal of Leukocyte Biology

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“…Neutrophils were prepared from bovine blood as described (13,23,24) and suspended at 37°C either in 137 mM NaCl/2.7 mM KCl/0.5 mM MgCl2/0.9 mM CaCl2/1.5 mM KH2PO4/8.1 mM Na2HPO4, pH 7.4 (phosphate-buffered saline) or in 120 mM NaCl/5 mM KCl/0.5 mM MgSO4/1 mM CaCl2/1 mM Na2HPO4/5 mM glucose/20 mM Hepes, pH 7.4 (Hepes medium). Neutrophil cytoplasts were obtained as described by Roos et al (19) after centrifugation of neutrophils at 33°C (30 min at 81,000 x g) through a discontinuous gradient of Ficoll containing 5 A.M cytochalasin B.…”

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confidence: 99%

Monitoring of cytosolic free Ca2+ in C5a-stimulated neutrophils: loss of receptor-modulated Ca2+ stores and Ca2+ uptake in granule-free cytoplasts.

Gennaro¹,

Pozzan²,

Romeo³

1984

Proc. Natl. Acad. Sci. U.S.A.

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“…Catalase activity and guaiacol peroxidase activity were measured as previously described (13,14). Glutathione peroxidase and glutathione reductase were measured according to the method of Reed (15), as modified by Gennaro et al ( 16). To test the influence of 1,3-bis(chloroethyl)-I-nitrosourea (BCNU) on glutathione reductase activity, 107 MEC were incubated for 10 min at 37'C in 1 ml KRP containing 5.5 mM glucose and 100 ug/ml BCNU or 0.1% ethanol control.…”

Section: Introductionmentioning

confidence: 99%

Neutrophil-endothelial cell interaction. Evidence for and mechanisms of the self-protection of bovine microvascular endothelial cells from hydrogen peroxide-induced oxidative stress.

Dobrina¹,

Patriarca²

1986

J. Clin. Invest.

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MethodsBovine microvascular endothelial cells (MEC) were able to degrade the H202 generated by phorbol myristate acetate-activated bovine neutrophils or by glucose oxidase with a maximal capacity of 4.0±1.2 (SD) nmol/10' cells/min, corresponding to the H202 released by about 3 X 10' neutrophils. H202 degradation occurred via the glutathione redox cycle and catalase. Degradation via the glutathione redox cycle was coupled with a marked stimulation of the hexose monophosphate shunt activity. The effect of H202 on ethidium bromide exclusion and on succinate oxidation was studied. Neither parameter was altered when MEC were exposed to H202 produced at rates within their degradative capacity. As soon as this was exceeded, impairment of both functions occurred. It is concluded that endothelial cells can protect themselves from H20rinduced i 'ury in a well-defined range of environmental H202 concentrations by actively degrading the peroxide.

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Biochemical Properties of Bovine Granulocytes

Cited by 48 publications

References 0 publications

Platelet-activating factor induces a concentration-dependent spectrum of functional responses in bovine neutrophils

Platelet-activating factor induces a concentration-dependent spectrum of functional responses in bovine neutrophils

Monitoring of cytosolic free Ca2+ in C5a-stimulated neutrophils: loss of receptor-modulated Ca2+ stores and Ca2+ uptake in granule-free cytoplasts.

Neutrophil-endothelial cell interaction. Evidence for and mechanisms of the self-protection of bovine microvascular endothelial cells from hydrogen peroxide-induced oxidative stress.

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