Biochemical quality of marine unicellular algae with special emphasis on lipid composition. II. Nannochloropsis sp.

Sukenik, Assaf; Zmora, Odi; Carmeli, Yael

doi:10.1016/0044-8486(93)90328-v

Cited by 193 publications

(95 citation statements)

References 31 publications

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“…This is consistent with known models of photo-oxidative stress [5]. As Sukenik [13] demonstrated, the C16 fatty acids are the main components of triacylglycerols (TAGs) of Nannochloropsis sp. and TAGs are known to increase and become richer in C16 fatty acids with high light.…”

Section: Light Effectssupporting

confidence: 70%

“…Since biomass harvest is most efficient in high-density cultures, condition changes from Figure 3(a) to 3(b)]. As the fraction of EPA among lipid classes changes little with illumination according to Sukenik [13], the relative increase in EPA was likely a result of the decrease in TAG, which contains little EPA. As cultures reached high densities and all cells were exposed to similar low average light intensities, the fatty acid composition converged in all bioreactors, as oleic acid became more prevalent than EPA [ Figure 3(c)].…”

Section: Light Effectsmentioning

confidence: 99%

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Effects of Light and Temperature on Fatty Acid Production in Nannochloropsis Salina

et al. 2012

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Accurate prediction of algal biofuel yield will require empirical determination of physiological responses to the environment, particularly light and temperature. One strain of interest, Nannochloropsis salina, was subjected to ranges of light intensity (5-850 μmol m −2 s −1 ) and temperature (13-40 °C) and its exponential growth rate, total fatty acids (TFA) and fatty acid composition were measured. The maximum acclimated growth rate was 1.3 day −1 at 23 °C and 250 μmol m −2 s −1 . Fatty acids were detected by gas chromatography with flame ionization detection (GC-FID) after transesterification to corresponding fatty acid methyl esters (FAMEs). A sharp increase in TFA containing elevated palmitic acid (C16:0) and palmitoleic acid (C16:1) during exponential growth at high light was observed, indicating likely triacylglycerol accumulation due to photo-oxidative stress. Lower light resulted in increases in the relative abundance of unsaturated fatty acids; in thin cultures, increases were observed in palmitoleic and eicosapentaenoic acids (C20:5ω3). As cultures aged and the effective light intensity per cell converged to very low levels, fatty acid profiles became more similar and there was a notable increase of oleic acid (C18:1ω9). The amount of unsaturated fatty acids was inversely proportional to temperature, demonstrating physiological adaptations to increase membrane fluidity. These data will improve prediction of fatty acid characteristics and yields relevant to biofuel production. OPEN ACCESSEnergies 2012, 5 732

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Section: Light Effectssupporting

confidence: 70%

Section: Light Effectsmentioning

confidence: 99%

Effects of Light and Temperature on Fatty Acid Production in Nannochloropsis Salina

et al. 2012

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“…3, Sigma-Aldrich). Total carbohydrate levels were determined by colorimetry using phenol and sulfuric acid [30]; following acid hydrolysis (2.5 M HCl at 80-90°C for 3 h; [31]). Final results were determined against a dextrose standard curve (0-100 μgm L −1 ; D-glucose, solid, N99% pure, Sigma-Aldrich, Cat.…”

Section: −3mentioning

confidence: 99%

Biochemical characterization of microalgal biomass from freshwater species isolated in Alberta, Canada for animal feed applications

et al. 2015

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“…Seaweeds are rich in vitamins, polysaccharides, and various functional polyphenols (Lordan et al 2011). Therefore, and not surprisingly, they have been identified as a rich source of bioactive compounds (Dykens et al 1992, Sukenik et al 1993, Matsukawa et al 1997.…”

Section: Isolation Of Dieckol From Ecklonia Cavamentioning

confidence: 99%

Thermostability of a marine polyphenolic antioxidant dieckol, derived from the brown seaweed Ecklonia cava

Kang¹,

Kim²,

Kang³

et al. 2012

ALGAE

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The thermostability of antioxidant activity of dieckol, a phlorotannin isolated from brown seaweed Ecklonia cava was investigated. The thermostable antioxidant properties of dieckol were evaluated at 30, 60, and 90°C for 7 days using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activities, and comparing its performance to that of ascorbic acid. The intracellular reactive oxygen species (ROS) scavenging activity and apoptotic body formation were investigated using DCF-DA assay and nuclear staining with Hoechst 33342, propidium iodide and flow cytometry. Dieckol treated at different temperatures during 7 days showed stable scavenging activities on towards DPPH and hydroxyl radicals. In addition, dieckol showed a stable protective effect against H 2 O 2 -induced apoptotic body formation in Vero cells. On the other hand, the radical scavenging activities and intracellular ROS scavenging activities of ascorbic acid, used as a positive control, were significantly decreased at 60°C and 90°C from on the 4th day and 3rd days, respectively. In conclusion, the results indicated that food grade antioxidant extracts containing dieckol derived from E. cava remain a stable during the temperatures encountered during the processing of food and cosmetics.

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Biochemical quality of marine unicellular algae with special emphasis on lipid composition. II. Nannochloropsis sp.

Cited by 193 publications

References 31 publications

Effects of Light and Temperature on Fatty Acid Production in Nannochloropsis Salina

Effects of Light and Temperature on Fatty Acid Production in Nannochloropsis Salina

Biochemical characterization of microalgal biomass from freshwater species isolated in Alberta, Canada for animal feed applications

Thermostability of a marine polyphenolic antioxidant dieckol, derived from the brown seaweed Ecklonia cava

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