Biochemical responses in farmed mussel Perna perna transplanted to contaminated sites on Santa Catarina Island, SC, Brazil

Bainy, Afonso Celso Dias; Almeida, Eduardo Alves de; Müller, Isabel Cristina; Ventura, Eliana Cristina; Medeiros, Igor Dias

doi:10.1016/s0141-1136(00)00039-8

Cited by 46 publications

(20 citation statements)

References 7 publications

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“…They were taken to laboratory and immediately used. Perna perna is commonly used in experiments regarding biomarkers and bioaccumulation (Bainy et al, 2000;Zaroni et al, 2001;Ferreira et al, 2000) and there is a protocol for employing its hemocytes in studies using the NRRT assay (Abessa et al, 2005). For this study, 20 animals were used per treatment.…”

Section: Neutral Red Retention Time Assaymentioning

confidence: 99%

“…Extracted from Google Earth. have used biomarkers (Bainy et al, 2000), which might be costly or require laboratorial efforts that are normally time consuming. The Neutral Red Retention Time (NRRT) assay has been used in studies concerning to evaluate the lysosomal membrane integrity, which can be used as a broad indicator of exposure to xenobiotics (Moore, 1990;Lowe et al, 1995;Cheung et al, 1997;Zaroni et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Using rapid assessment of marine pollution (RAMP) techniques to assess the quality of marine sediments

Abessa

Bı́cego²,

Sousa³

et al. 2024

Ecotoxicol. Environ. Contam.

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Sediments represent an important environmental compartment, because they provide substrate for a range of species and may accumulate contaminants in high concentrations. However, the universe of methods to assess the quality of sediments is still small. This investigation aimed to assess the quality of sediments from some sites of Ubatuba (SE Brazil). To achieve that, sediments were analyzed for the presence of PAHs, by immunoassay ELISA kit for the carcinogenic PAH (c-PAH) RaPID Assay, and for toxicity to amphipods, sea-urchin embryos and direct exposure of mussel hemocytes and measurements of the nuetral red retention time (NRTT). Results showed higher levels of PAHs in sediments from Itagua and Ribeira, those more intensely affected by contamination sources. The ecotoxicological analyses indicated these two sites as more degraded, together with the sediments from Lamberto Beach, which is also influenced by nautical activities. The NRRT correlated with the quantities of PAHs in sediments. We concluded that Ribeira Bay and Itaguá Beach need more detailed investigation on pollution and that the NRRT assay exposing directly hemocytes to sediment elutriates can be a useful tool to assessing sediment quality.

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Section: Neutral Red Retention Time Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Using rapid assessment of marine pollution (RAMP) techniques to assess the quality of marine sediments

Abessa

Bı́cego²,

Sousa³

et al. 2024

Ecotoxicol. Environ. Contam.

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“…Detoxification enzymes are used widely to detect pollution in aquatic environments, and include the glutathione-stransferases (GSTs) which comprising a superfamily of multifunctional proteins with fundamental roles in the detoxification of a wide range of compounds (Frova, 2006, Davies, 1985, Bainy et al, 2000. Additionally, peroxidase and isomerase activities have been described for GST enzymes, enabling their use as biomarkers for oxidative stress (Sheehan et al, 2001).…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Biological responses to contaminants in the Humber Estuary: Disentangling complex relationships

García‐Alonso

Greenway

Munshi

et al. 2011

Marine Environmental Research

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Due to the ecological importance of estuaries, it is necessary to understand the biological effects that potentially toxic contaminants induce in bioindicator species. A key aspect is whether effects at lower levels of biological organisation transfer through the system to higher levels. In understanding such processes, characterising multivariate relationships between contaminants, sediment toxicities and detoxification processes are important. Worms (Hediste diversicolor) and sediments were collected along the Humber Estuary, England, and inorganic and organic contaminants were quantified. Sediment toxicities and glutathione S-transferases (GSTs) activity in the ragworm were analysed. Concentrations of metals were highest near urban and industrial areas, whereas organic contaminants appeared at upstream locations. GST activity correlated with heavy metals. The genotoxicity, estrogenicity, dioxin and dioxin-like activity were higher at upstream locations. Estrogenicity correlated with alkylphenols and some organochlorines, whilst genotoxicity correlated with organochlorines and heavy metals. Despite this, higher level biological responses could not be predicted, indicating that homeostasis is operating.

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“…Multi-level studies can produce more reliable information on the conditions of the studied organisms and provide the data necessary for the understanding of the mode of action of pollutants, as well as the mechanistic comprehension of the processes related to intoxication and detoxication, which is especially desirable when assessing the early signs of stress. In Brazil, the use of biomarkers in environmental evaluations is recent (Bainy et al, 2000). Few laboratories have the appropriate equipment and trained staff to perform such assays.…”

Section: Introductionmentioning

confidence: 99%

“…As a suspension feeder, P. perna may accumulate chemical compounds in its tissues. In Brazil, it has been used in studies regarding effects of xenobiotics at the biochemical (Bainy et al, 2000) and cellular levels (Zaroni et al, 2001), and also in bioaccumulation studies (Ferreira et al, 2000). Recently, within the project "Early Warning, Chemical and Biological Markers of Pollution in Coastal Waters", a collaboration agreement was established between the University of Plymouth and the Oceanographic Institute of the University of São Paulo, in which some newly validated techniques for the cardiac activity assessment -CAPMON (Depledge and Andersen, 1990) and the Neutral Red retention assay (Moore, 1990) were introduced in Brazil, together with the necessary equipment and training.…”

Section: Introductionmentioning

confidence: 99%

Physiological and cellular responses in two populations of the mussel Perna perna collected at different sites from the coast of São Paulo, Brazil

Abessa

Zaroni

Sousa

et al. 2005

Braz. arch. biol. technol.

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The physiological conditions of mussels from Ubatuba and Santos and also of organisms transplanted from Ubatuba to Santos were studied by using different techniques. Assays for lysosomal stability were conducted on the haemolymph. Heart rate activity was monitored for 6h. The embryonic development of larvae obtained from the collected mussels was analysed. For all the compared groups of mussels, no significant differences were observed for the cardiac activity monitoring and the embryonic bioassays. The mean Neutral Red (NR) retention time was similar for the animals from Santos and Ubatuba, whereas the organisms transplanted to Santos showed a reduction in the retention time of the dye, indicating damage in the lysosomal membranes. These differences were possibly due to environmental factors, but further investigations are required to confirm this hypothesis.

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Biochemical responses in farmed mussel Perna perna transplanted to contaminated sites on Santa Catarina Island, SC, Brazil

Cited by 46 publications

References 7 publications

Using rapid assessment of marine pollution (RAMP) techniques to assess the quality of marine sediments

Using rapid assessment of marine pollution (RAMP) techniques to assess the quality of marine sediments

Biological responses to contaminants in the Humber Estuary: Disentangling complex relationships

Physiological and cellular responses in two populations of the mussel Perna perna collected at different sites from the coast of São Paulo, Brazil

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