Biochemical responses of freshwater mussel Unio tumidus to titanium oxide nanoparticles, Bisphenol A, and their combination

Gnatyshyna, Lesya; Falfushynska, Halina; Horyn, Oksana; Khoma, Vira; Martinyuk, Viktoria; Mishchuk, Olena; Mishchuk, N. A.; Stoliar, Оksana

doi:10.1007/s10646-019-02090-6

Cited by 33 publications

(8 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, in the mussel Unio tumidus exposure to 75 nm nano-TiO 2 (100 µg L −1 for 14 days) suppressed the SOD activity in the digestive glands. [116] In R. decussatus exposure to 12.5 nm nano-TiO 2 (100 µg L −1 for 14 days) elevated the SOD level in the digestive glands. [112] Similarly, following exposure to 55 nm nano-TiO 2 (1 mg L −1 ), the level of SOD in several bivalves was decreased, while exposure to 20-21nm nano-TiO 2 stimulated the SOD activity.…”

Section: Mollusksmentioning

confidence: 89%

“…[114,115] In the freshwater mussel Unio tumidus, exposure to nano-TiO 2 resulted in elevated levels of ROS, depletion of GSH and activation of SOD. [116] Increased lipid peroxidation (indicated by MDA accumulation) was found in the nano-TiO 2 exposed clams R. decussatus, C. fluminea, oysters Crassostrea virginica, and abalone Haliotis diversicolor . [86,102,112,117] The intensity of oxidative stress responses of mollusks exposed to nano-TiO 2 appears to depend on the size of nano-TiO 2 .…”

Section: Mollusksmentioning

confidence: 95%

See 1 more Smart Citation

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Luo

Xie

et al. 2020

Small

133

View full text Add to dashboard Cite

Titanium dioxide nanoparticles (nano‐TiO2) are widely used in consumer products, raising environmental and health concerns. An overview of the toxic effects of nano‐TiO2 on human and environmental health is provided. A meta‐analysis is conducted to analyze the toxicity of nano‐TiO2 to the liver, circulatory system, and DNA in humans. To assess the environmental impacts of nano‐TiO2, aquatic environments that receive high nano‐TiO2 inputs are focused on, and the toxicity of nano‐TiO2 to aquatic organisms is discussed with regard to the present and predicted environmental concentrations. Genotoxicity, damage to membranes, inflammation and oxidative stress emerge as the main mechanisms of nano‐TiO2 toxicity. Furthermore, nano‐TiO2 can bind with free radicals and signal molecules, and interfere with the biochemical reactions on plasmalemma. At the higher organizational level, nano‐TiO2 toxicity is manifested as the negative effects on fitness‐related organismal traits including feeding, reproduction and immunity in aquatic organisms. Bibliometric analysis reveals two major research hot spots including the molecular mechanisms of toxicity of nano‐TiO2 and the combined effects of nano‐TiO2 and other environmental factors such as light and pH. The possible measures to reduce the harmful effects of nano‐TiO2 on humans and non‐target organisms has emerged as an underexplored topic requiring further investigation.

show abstract

Section: Mollusksmentioning

confidence: 89%

Section: Mollusksmentioning

confidence: 95%

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Luo

Xie

et al. 2020

Small

133

View full text Add to dashboard Cite

show abstract

“…The climate changes can add unpredictable consequences to the biological effect of waterborne chemical substances, in particular associated with the local extreme events that are likely to occur in summer in most rivers [3,4]. Therefore, the model experiments with subjecting of the organism to single suspected adverse effect in the optimal conditions can give distorted evidence of its ability to withstand this effect [2][3][4][5].…”

mentioning

confidence: 99%

Metallothioneins contribution to the response of bivalve mollusk to xenobiotics

Khoma¹,

Gnatyshyna²,

Мартынюк³

2020

Ukr.Biochem.J

View full text Add to dashboard Cite

Estimation of cellular thiols metallothioneins (mts) sensitivity to continuous pressure of environmental chemical 'cocktail' of xenobiotics needs investigation in correct model experiments. the aim of this study was to elucidate mts contribution into bivalve mollusk response to co-exposure to xenobiotics and elevated temperature. We treated the mussels Unio tumidus Philipson, 1788 (Unionidae) with drugs diclofenac (Dc, 2 nm), nifedipine (Nf, 2 nm) or with organophosphonate herbicide glyphosate (Gl, formulation roundup maX, 79 nm) separately at 18°c and in combination at 18°c (DcNfGl) and 25°c (DcNfGl+t) during 14 days. mts were isolated from digestive gland by size-exclusion chromatography. the concentration of mts in the tissue was assessed according to metals (Zn, cu, cd) in the eluted peak of mts (mt-me) and thiols (mt-Sh) content. tissue redox status was assessed using lactate/pyruvate ratio. the assay of cells viability was based on the lysosomes ability of hemocytes to concentrate the Neutral red (Nr) dye. It was found, that mt-Sh content in the digestive gland was increased under all exposures. treatment with Dc increased the level of mt-me, whereas treatment with Gl decreased it and increased lactate/pyruvate ratio. Nf decreased this ratio by elevating pyruvate level and increased lysosomal membrane stability in hemocytes. at co-exposure to xenobiotics and elevated temperature the number of hemocytes with nuclear abnormalities was increased indicating the exceeding of organisms' adaptive limits. multivariate statistical analyses showed negative correlations in pairs mt-Sh/mt-me and mt-Sh/pyruvate and distinguished Gl and DcNfGl+t exposed groups from other groups.

show abstract

“…Instead, an antiestrogenic potential of TAM (0.1, 1, 10, 50 μg/L) was recorded in sexually undifferentiated freshwater clams (Corbicula fluminea), as reduced levels of VTG were found in treated animals when compared to controls (Aguirre-Martínez et al 2018). Exposure for 14 days to titanium oxide nanoparticles (TiO 2 1.25 μM, corresponding to 100 μg/L), bisphenol A (BPA 0.88 nM, corresponding to 200 ng/L), and their combination (TiO 2 + BPA) caused a significant increase in ALP levels in gonads from U. tumidus (Gnatyshyna et al 2019). However, in that study, the authors stated that increases in ALP levels in the gonads of freshwater mussels cannot be solely attributed to BPA, but also to increased supply of the gonad activity with phospholi-poproteins and Zn (Gnatyshyna et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Exposure for 14 days to titanium oxide nanoparticles (TiO 2 1.25 μM, corresponding to 100 μg/L), bisphenol A (BPA 0.88 nM, corresponding to 200 ng/L), and their combination (TiO 2 + BPA) caused a significant increase in ALP levels in gonads from U. tumidus (Gnatyshyna et al 2019). However, in that study, the authors stated that increases in ALP levels in the gonads of freshwater mussels cannot be solely attributed to BPA, but also to increased supply of the gonad activity with phospholi-poproteins and Zn (Gnatyshyna et al 2019). In M. galloprovincialis, a 2fold increase in ALP levels was recorded in males after 14 days of exposure to 250 ng/L of ibuprofen (Gonzalez-Rey and Bebianno 2012).…”

Section: Introductionmentioning

confidence: 99%

Molecular and biochemical responses of vitellogenin in the mussel Mytilus galloprovincialis exposed to the glyphosate-based herbicide Roundup® Power 2.0

Fabrello

Grapputo

Munari

et al. 2020

Environ Sci Pollut Res

View full text Add to dashboard Cite

Glyphosate-based herbicides (GBHs) occur in aquatic ecosystems at concentrations of hundreds of micrograms per liter. As formulation adjuvants are suspected to be endocrine-disrupting chemicals, we assessed the effects of the recent GBH formulation Roundup® Power 2.0 on vitellogenin (VTG) in Mytilus galloprovincialis. Mussels were exposed for 7, 14, and 21 days to two concentrations of the commercial formulation, corresponding to 100 and 1000 μg/L of glyphosate. The expression of the vtg gene in gonads of females and males, as well as the levels of alkali labile phosphates (ALP) in gonads and non-gonadal tissues from the two sexes were measured. No significant alterations were observed in vtg expression values during the exposure. Conversely, a significant reduction in gonadal ALP levels was observed in females exposed for 21 days and in males exposed for 7 days. In addition, ALP levels increased significantly in gonads from males exposed for 21 days to the two concentrations of Roundup®. As for non-gonadal tissues, ALP levels did not change significantly in females, whereas ALP levels decreased significantly in non-gonadal tissues from males exposed for 21 days to the lowest concentration tested. An overall statistically significant difference in ALP levels was found between females and males. Although preliminary, our study suggests that GBH can affect reproduction-related parameters in mussels.

show abstract

Biochemical responses of freshwater mussel Unio tumidus to titanium oxide nanoparticles, Bisphenol A, and their combination

Cited by 33 publications

References 92 publications

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Metallothioneins contribution to the response of bivalve mollusk to xenobiotics

Molecular and biochemical responses of vitellogenin in the mussel Mytilus galloprovincialis exposed to the glyphosate-based herbicide Roundup® Power 2.0

Contact Info

Product

Resources

About

Biochemical responses of freshwater mussel Unio tumidus to titanium oxide nanoparticles, Bisphenol A, and their combination

Cited by 33 publications

References 92 publications

Rethinking Nano‐TiO2 Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Rethinking Nano‐TiO2 Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Metallothioneins contribution to the response of bivalve mollusk to xenobiotics

Molecular and biochemical responses of vitellogenin in the mussel Mytilus galloprovincialis exposed to the glyphosate-based herbicide Roundup® Power 2.0

Contact Info

Product

Resources

About

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals

Rethinking Nano‐TiO₂ Safety: Overview of Toxic Effects in Humans and Aquatic Animals