Biochemical side effects of genetic transformation of pineapple

Yabor, Lourdes; Arzola, Mayda; Aragón, C.; Hernández, Martha; Arencibia, Ariel D.; Lorenzo, José Carlos

doi:10.1007/s11240-006-9097-z

Cited by 22 publications

(9 citation statements)

References 33 publications

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“…Genetic transformation can produce unintended pleiotropic effects on occasion (Yabor et al, 2006;Abdeen and Miki, 2009) that negatively affect plant phenotype. In tobacco (Nicotiana tabacum), for example, stilbene overexpression led to male sterility (Fischer et al, 1997;Hö fig et al, 2006), probably due to shortages of substrates for flavonoid and sporopollenin production.…”

Section: Genetic Transformation Of Spruce With Pasts Provided New Insmentioning

confidence: 99%

Biosynthesis of the Major Tetrahydroxystilbenes in Spruce, Astringin and Isorhapontin, Proceeds via Resveratrol and Is Enhanced by Fungal Infection

et al. 2011

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V6T 1Z4 (S.G.R., J.B.)Stilbenes are dibenzyl polyphenolic compounds produced in several unrelated plant families that appear to protect against various biotic and abiotic stresses. Stilbene biosynthesis has been well described in economically important plants, such as grape (Vitis vinifera), peanut (Arachis hypogaea), and pine (Pinus species). However, very little is known about the biosynthesis and ecological role of stilbenes in spruce (Picea), an important gymnosperm tree genus in temperate and boreal forests. To investigate the biosynthesis of stilbenes in spruce, we identified two similar stilbene synthase (STS) genes in Norway spruce (Picea abies), PaSTS1 and PaSTS2, which had orthologs with high sequence identity in sitka (Picea sitchensis) and white (Picea glauca) spruce. Despite the conservation of STS sequences in these three spruce species, they differed substantially from angiosperm STSs. Several types of in vitro and in vivo assays revealed that the P. abies STSs catalyze the condensation of p-coumaroyl-coenzyme A and three molecules of malonyl-coenzyme A to yield the trihydroxystilbene resveratrol but do not directly form the dominant spruce stilbenes, which are tetrahydroxylated. However, in transgenic Norway spruce overexpressing PaSTS1, significantly higher amounts of the tetrahydroxystilbene glycosides, astringin and isorhapontin, were produced. This result suggests that the first step of stilbene biosynthesis in spruce is the formation of resveratrol, which is further modified by hydroxylation, O-methylation, and O-glucosylation to yield astringin and isorhapontin. Inoculating spruce with fungal mycelium increased STS transcript abundance and tetrahydroxystilbene glycoside production. Extracts from STSoverexpressing lines significantly inhibited fungal growth in vitro compared with extracts from control lines, suggesting that spruce stilbenes have a role in antifungal defense.

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Section: Genetic Transformation Of Spruce With Pasts Provided New Insmentioning

confidence: 99%

Biosynthesis of the Major Tetrahydroxystilbenes in Spruce, Astringin and Isorhapontin, Proceeds via Resveratrol and Is Enhanced by Fungal Infection

et al. 2011

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“…As mentioned above, during hardening, leaf levels of malondialdehyde, other aldehydes, chlorophyll b, and total chlorophyll pigments decreased because of genetic transformation only (Yabor et al 2006). Such differences between transgenic and non-transgenic materials were not observed after 1 yr of field growth (Table 2).…”

Section: Resultsmentioning

confidence: 87%

“…Based on these prospects, we previously studied some of the biochemical side effects of pineapple genetic transformation in interaction with the herbicide FINALE ® during hardening (Yabor et al 2006;Yabor et al 2008). Levels of aldehydes and chlorophylls, and peroxidase activity were recorded in middle-aged leaves.…”

Section: Introductionmentioning

confidence: 99%

“…However, as expected, nontransgenic plants decreased levels of chlorophylls (a, b, total) after application of FINALE ® . The genetic transformation of pineapple with the bar gene not only conferred resistance to the herbicide FINALE ® but also promoted other biochemical changes during in vitro plantlet hardening (Yabor et al 2006;Yabor et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a field-grown transgenic pineapple clone containing the genes chitinase, AP24, and bar

Yabor

Valle

Carvajal

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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We previously introduced the bar gene, along with chitinase and AP24 genes, into the pineapple genome. The present report focuses on the evaluation of the first vegetative generation of a transgenic clone containing these genes. Three materials were compared: macropropagated controls (non-transformed), micropropagated controls (nontransformed), and micropropagated transformed plants. From each group, 50% of the plants were sprayed with FINALE ® 3 mo after the experiment initiation. The characterization was performed after 1 yr of field growth. FINALE ® killed all non-transgenic plants. Plants that survived the herbicide application showed 2n=50 chromosomes in their roots after 1 yr in the field. Micropropagated transformed plants sprayed with FINALE ® did not show phenotype differences from micropropagated transformed plants not sprayed with the herbicide. Between the micropropagated transformed plants sprayed with FINALE ® and the micropropagated control plants, the following differences were observed: modifications in levels of cell wall-linked, free and total phenolics, and total proteins. Moreover, changes of the fruit mass without crown were also recorded. Between the micropropagated transformed plants sprayed with FINALE ® and the macropropagated control plants, levels of chlorophyll b, total chlorophyll pigments, and proteins were different. Furthermore, activities of phenylalanine ammonia-lyase, superoxide dismutase, and glutamine synthetase were dissimilar. The plant height and diameter, and the crown height were also different. Until now, we have evaluated transformed pineapple plants during hardening and field growth. Although some unexpected variations were recorded, we believe they are not relevant enough to justify rejection of transgenesis as an important tool for pineapple genetic improvement.

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“…Regression analyses of the effects of sodium nitrate, potassium phosphate, magnesium sulfate, potassium chloride, and sucrose on banana leaf lesion area caused by Fusarium culture filtrates did not generate reasonable models (data in Companioni et al 2004). We also failed to find mathematical equations to establish the relationship between the time course and the levels of malondialdehyde, other aldehydes, chlorophyll pigments (a, b, total), phenolics (free and cell-wall linked) and protein in transgenic pineapple plantlets (data in Yabor et al 2006). Consequently, based on our experience, adequate regression analyses are not always obtained.…”

Section: Resultsmentioning

confidence: 96%

Use of regression analysis in plant cell, tissue, and organ culture experiments

Lorenzo

García-Borroto

2008

In Vitro Cell.Dev.Biol.-Plant

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Several authors have suggested that plant biotechnologists perform regression or trend analysis to compare means of related quantitative treatments (e.g., doses of inositol). The present paper compares two statistical strategies to determine the effect of inositol (0-400 mg l −1 ) on proteolytic activity in the culture medium during pineapple growth in temporary immersion bioreactors. Strategy 1 involved one-way analysis of variance (ANOVA) followed by Tukey's Honestly Significant Difference (HSD). Strategy 2 consisted in the development of different regression analyses to determine the best fit equations to describe the experimental results. Curvefit software (version 2.10-0, May 15, 1987, Thomas S. Cox) was used. Cauchy, Normal, Parabola, and Hoerl equations were the best fitted according to their determination coefficients (R 2 ). The optimal inositol concentrations to increase proteolytic activity were determined from the equations. Quite different results were obtained following strategy 2: 126.76 mg l −1 inositol from Cauchy, 131.29 mg l −1 from Normal, 145.06 mg l −1 from Parabola, and 14.05 mg l −1 from Hoerl equations. In contrast, experimental data identified 200 mg l −1 inositol as the most adequate concentration to increase proteolytic activity in the culture medium. The statistical strategy 1, one-way ANOVA followed by Tukey HSD clearly supported this biological observation. In this paper, regression analysis was not useful to describe our experimental results.

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Biochemical side effects of genetic transformation of pineapple

Cited by 22 publications

References 33 publications

Biosynthesis of the Major Tetrahydroxystilbenes in Spruce, Astringin and Isorhapontin, Proceeds via Resveratrol and Is Enhanced by Fungal Infection

Biosynthesis of the Major Tetrahydroxystilbenes in Spruce, Astringin and Isorhapontin, Proceeds via Resveratrol and Is Enhanced by Fungal Infection

Characterization of a field-grown transgenic pineapple clone containing the genes chitinase, AP24, and bar

Use of regression analysis in plant cell, tissue, and organ culture experiments

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