Biochemical Studies of Physiological Function of Tocopheronolactone. 1

Uchiyama, Mitsuru; Suzuki, Yasuo; Fukuzawa, Kenji

doi:10.1248/yakushi1947.88.6_678

Cited by 52 publications

(50 citation statements)

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“…HPLC analysis of the sugar fraction under the same conditions as in the case of that of 2 showed the presence of D-glucose (positive optical rotation). DPPH Radical Scavenging Activity DPPH radical scavenging activities of the EtOH extract and compounds were investigated according to the method of Uchiyama et al 11) Briefly, a solution of the test compound in EtOH (1.0 ml), acetate buffer (pH 5.5, 1.0 ml) and 200 mM DPPH radical in EtOH (0.5 ml) was incubated at room temperature for 30 min. Reduction of the DPPH radical was measured at 517 nm.…”

Section: Methodsmentioning

confidence: 99%

Constituents with Radical Scavenging Effect from Opuntia dillenii: Structures of New .ALPHA.-Pyrones and Flavonol Glycoside.

et al. 2002

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Section: Methodsmentioning

confidence: 99%

Constituents with Radical Scavenging Effect from Opuntia dillenii: Structures of New .ALPHA.-Pyrones and Flavonol Glycoside.

et al. 2002

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“…Examination of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Radical-Scavenging Activity DPPH radical-scavenging activity was investigated according to the method of Uchiyama et al 8) Briefly, a solution of sample in acetate buffer (pH 5.5, 2 ml) and ethanol (EtOH, 2 ml) was treated with 2ϫ10 Ϫ4 M DPPH (Nacalai Tesque) in EtOH solution (1 ml) and the mixture was incubated at room temperature for 30 min (final concentration of DPPH, 40 mM), and the absorbance change at 517 nm was measured. The reaction solution without DPPH was used as a blank test.…”

Section: )mentioning

confidence: 99%

“…8,12) When this compound accepts an electron or a hydrogen radical to become a more stable compound, its absorption vanishes. DPPH radical-scavenging activities of the phenolic constituents were examined.…”

Section: )mentioning

confidence: 99%

Hepatoprotective and Antioxidative Properties of Salacia reticulata: Preventive Effects of Phenolic Constituents on CCl4-Induced Liver Injury in Mice.

Yoshikawa

Ninomiya

Shimoda

et al. 2002

Biological & Pharmaceutical Bulletin

113

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The Hippocrateaceae 1) Salacia (S.) reticulata and S. oblonga, woody climbers distributed in Sri Lanka and the southern region of India, have been used in the treatment of rheumatism, gonorrhea, and skin diseases, particularly the initial stages of diabetes, in Sri Lankan traditional medicine.2) In India, these plants have been prescribed as an anodyne, antiinflammatory agent, and liver tonic, and also for wound healing and treatment of amenorrhea in traditional Indian preparations.3) Recently, we isolated potent a-glucosidase inhibitors called salacinol and kotalanol from the watersoluble portion of the roots and stems of S. reticulata 4) and S. oblonga 5) as antidiabetogenic principles, and characterized their unique spiro-like inner salt structure composed of 1-deoxy-4-thio-D-arabinofuranosyl sulfonium cation and 1-deoxysugar alcohol sulfate anion.4) Furthermore, we found that the triterpene and diterpene constituents from the ethyl acetate-soluble portion of S. oblonga showed aldose reductase inhibitory activity, 5) which is involved in the development of diabetogenic complications, while the polyphenol constituents from S. reticulata inhibited a-glucosidase and aldose reductase.6) However, the other traditional effects and their pharmacologically active constituents were left uncharacterized. In particular, although S. reticulata is used as a liver tonic, no scientific evidence has been reported.As a continuing study, we found that the water-soluble (SRHW) and methanol-soluble (SRM) extracts showed inhibitory effects against CCl 4 -induced liver injury in mice. In this report, we describe the hepatoprotective and antioxidative effects of S. reticulata and its active constituents. MATERIALS AND METHODS AnimalsMale ddY mice were purchased from Kiwa Laboratory Animal Co., Ltd. (Wakayama, Japan). The animals were maintained at a constant temperature of 23Ϯ2°C and were fed standard laboratory chow (MF, Oriental Yeast Co., Ltd., Tokyo, Japan) and tap water.Materials Preparation of SRHW and SRM extracts, and determination of amount of phenolic compounds: Dried roots and stems of S. reticulata obtained from Sri Lanka and India were crushed and extracted with 5 times the volume of water (H 2 O) at 80°C for 3 h. The solvent was evaporated under reduced pressure at 40°C to give the SRHW extract. The residue was continuously extracted with 4 times the volume of MeOH for 3 h and the SRM extract was obtained. The amount of phenolic compounds was determined by the colorimetric method using a ferrous tartrate. Briefly, each extract dissolved in H 2 O (1 mg/ml) was mixed with 5 ml of ferrous tartrate reagent [FeSO 4 · 7H 2 O (1 mg/ml) and C 4 H 4 KNaO 6 · 4H 2 O (5 mg/ml) dissolved in H 2 O]. This was added to 15 ml of Sörensen's phosphate buffer (67 mM Na 2 HPO 4 , 67 mM KH 2 PO 4 , pH 7.5) and shaken. The absorbance of the mixture was measured at 540 nm. The amount of phenolic constituents was calculated using the calibration curve of ethyl gallate solution as a standard. (6) The hepatoprotective effects of the hot water...

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“…Assay of Scavenging Effect on DPPH The method of Uchiyama et al 19) was applied in a slightly modified manner. The EtOH solution (1.00 ml) of each test sample was added to a mixture of 0.1 M acetic acid buffer (pH 5.5, 1.00 ml) and 0.5 mM DPPH EtOH solution (0.50 ml) in a test tube and left to stand at room temperature for 30 min.…”

mentioning

confidence: 99%

Two New Steroid Glycosides and a New Sesquiterpenoid Glycoside from the Underground Parts of Trillium kamtschaticum

Ono

Takamura

Sugita

et al. 2007

CHEMICAL & PHARMACEUTICAL BULLETIN

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Two new steroid glycosides, named trikamsterosides A and B, and a new sesquiterpenoid glycoside named trikamsesuquiside A were isolated from the underground parts of Trillium kamtschaticum PALL. along with 18 known compounds comprising 12 steroids, one sesquiterpenoid glycoside, one phenylpropanoid, one flavonoid glycoside, and three phenylpropanoid sucrose esters. Their chemical structures were determined on the basis of spectroscopic data and chemical evidence. Among them, one phenylpropanoid sucrose ester showed almost the same radical-scavenging effect on 1,1-diphenyl-2-picrylhydrazyl as that of a a-tocopherol.

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Biochemical Studies of Physiological Function of Tocopheronolactone. 1

Cited by 52 publications

References 0 publications

Constituents with Radical Scavenging Effect from Opuntia dillenii: Structures of New .ALPHA.-Pyrones and Flavonol Glycoside.

Constituents with Radical Scavenging Effect from Opuntia dillenii: Structures of New .ALPHA.-Pyrones and Flavonol Glycoside.

Hepatoprotective and Antioxidative Properties of Salacia reticulata: Preventive Effects of Phenolic Constituents on CCl4-Induced Liver Injury in Mice.

Two New Steroid Glycosides and a New Sesquiterpenoid Glycoside from the Underground Parts of Trillium kamtschaticum

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