Biochemical studies on native and cross-linked aggregates of Aspergillus awamori feruloyl esterase

Preparation of Cross-Linked Enzyme Aggregates (CLEAs) of an Inulosucrase Mutant for the Enzymatic Synthesis of Inulin-Type Fructooligosaccharides

“…It can be seen that the precipitated enzyme can retain 85.5 ± 9.4% of its initial enzyme activity using 40% (w/v) of ammonium sulfate ( Figure 1B). These results are consistent with earlier reports that ammonium sulfate has been used to prepare a high enzyme activity of various CLEAs, such as α-amylase [22], β-galactosidase [23], lipase [28], tyrosinase [29,30], subtilisin [31], phytase [32], feruloyl esterase [33], invertase [34,35], and levansucrase [24].…”

Expression of feruloyl esterase from Aspergillus awamori in Escherichia coli: Characterization and crystal studies of the recombinant enzyme

“…The values are similar to that reported previously in the literature [13][14][15][16][17][18][19] for the purified native A. awamori feruloyl esterase (FAE-II) enzyme. The K m and V max values were observed to be 1.38 mM and 22.63 mol min −1 , respectively.…”

“…To understand both the catalytic mechanism and the exquisite substrate recognition displayed by these enzymes, the structure of recombinant Aspergillus awamori feruloyl esterase catalytic module should be investigated by enzymology researchers. In continuation of our research programs oriented toward the study of feruloyl esterase [11][12][13][14][15][16][17][18][19][20], the aim of the present work is to produce the enzyme FAE-A using recombinant DNA technology, characterize the rDNA enzyme and compare it with the wild type one. In this study, the rDNA enzyme was successfully crystallized and some conclusions from the X-ray data were obtained.…”

Cross-Linked Enzyme Aggregates of Naringinase: Novel Biocatalysts for Naringin Hydrolysis

“…Therefore, the technology of CLEAs an alternative immobilization procedure has several advantages in the industrial context and appears as a simple, economical, and easy optimization for the immobilization of enzymes, allowing the selection and retention of enzymatic activity in aqueous media and within a wide range of pH and temperature [5, 7, 8]. …”