Biochemical, Transcriptional and Translational Evidences of the Phenol-meta-Degradation Pathway by the Hyperthermophilic Sulfolobus solfataricus 98/2

Comte, Alexia; Christen, Pierre; Davidson, Sylvain; Pophillat, Matthieu; Lorquin, Jean; Auria, Richard; Simon, Gwenola; Casalot, Laurence

doi:10.1371/journal.pone.0082397

Cited by 19 publications

(17 citation statements)

References 40 publications

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“…Separation was achieved with an LC column Waters XTerra C18 column, eluted with a gradient of acetonitrile in water containing acetic acid (0.1% v/v); from 0 to 40% acetonitrile, using the following parameters-ionization: electro spray positive (ES+), low rate: 0.15 ml/min. For mass spectrometer, the following parameters were used: desolvation gas: 550 L/h; cone gas: 30 L/h; desolvation temperature: 250°C; source temperature: 110˚C; capillary voltage: 3000 V; cone voltage: 30 V; collision energy: 4 eV; nebulize gas: nitrogen 30 ml/min; collision gas: argon 0.5 µl /min (Comte et al, 2013;and Glish and Vachet, 2003).…”

Section: Phenol Estimation By Analytical Methods and Lc-ms Analysismentioning

confidence: 99%

Biodegradation of phenol by free and immobilized Candida tropicalis NPD1401

Kumar¹,

Neeraj²,

Mishra³

et al. 2018

Afr. J. Biotechnol.

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The present research aimed to evaluate the free and immobilized cell of Candida tropicalis NPD1401 for phenol degradation. Immobilized cell of C. tropicalis degraded efficiently up to 98% at a concentration of 1000 mg/l of phenol whereas free cells degraded up to 63% of the same concentration under 9 days of incubation. Stored immobilized beads were reused after 15 days and found to have successfully degraded 62.1% of phenol in the mineral salt medium (MSM). Growth of C. tropicalis was observed in the phenol containing medium by measuring the dry weight of biomass (0.89 g/l at concentration 1000 mg/l) and the degradation was monitored using analytical techniques. Liquid chromatography-mass spectroscopy (LC-MS) analysis confirmed that phenol was degraded by ortho-pathways by the finding of metabolite cis, cis-muconic acid, phenyl phosphate and catechol. Next, isolated strain was identified on the basis of PCR amplification of sequence D2 region of the large subunit of 28S rDNA and it was confirmed as C. tropicalis. By observing the efficiency of the isolate it may be used for the further bioremediation purpose of the phenol contaminated site in the environments.

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Section: Phenol Estimation By Analytical Methods and Lc-ms Analysismentioning

confidence: 99%

Biodegradation of phenol by free and immobilized Candida tropicalis NPD1401

Kumar¹,

Neeraj²,

Mishra³

et al. 2018

Afr. J. Biotechnol.

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“…Most microorganisms usually degrade phenol through the aerobic pathway in which the phenol hydroxylase catalyzes phenol to catechol, which is the first and key step of phenol degradation and often directly affects the degradation rate of phenol. Then, this pathway can be divided into the ortho-pathway and meta-pathway, which are two separate metabolic systems according to the opening mode of the aromatic ring[ 38 , 39 ]. Interestingly, different kinds and even concentrations of aromatic hydrocarbons can activate different metabolic pathways of some certain microorganisms, with Pseudomonas cepacia as an example in which salicylate can only activate the ortho-pathway while benzoate can activate both the ortho-pathway and meta-pathway[ 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Degradation of phenol via ortho-pathway by Kocuria sp. strain TIBETAN4 isolated from the soils around Qinghai Lake in China

Ali

Liu

et al. 2018

PLoS ONE

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Based on the feature of high-altitude permafrost topography and the diverse microbial ecological communities of the Qinghai-Tibetan Plateau, soil samples from thirteen different collection points around Qinghai lake were collected to screen for extremophilic strains with the ability to degrade phenol, and one bacterial strain recorded as TIBETAN4 that showed effective biodegradation of phenol was isolated and identified. TIBETAN4 was closely related to Kocuria based on its observed morphological, molecular and biochemical characteristics. TIBETAN4 grew well in the LB medium at pH 7–9 and 0–4% NaCl showing alkalophilicity and halophilism. The isolate could also tolerate up to 12.5 mM phenol and could degrade 5 mM phenol within 3 days. It maintained a high phenol degradation rate at pH 7–9 and 0–3% NaCl in MSM with 5 mM phenol added as the sole carbon source. Moreover, TIBETAN4 could maintain efficient phenol degradation activity in MSM supplemented with both phenol and glucose and complex water environments, including co-culture Penicillium strains or selection of non-sterilized natural lake water as a culture. It was found that TIBETAN4 showed enzymatic activity of phenol hydroxylase and catechol 1,2-dioxygenase after induction by phenol and the corresponding genes of the two enzymes were detected in the genome of the isolate, while catechol 2,3-dioxygenase or its gene was not, which means there could be a degradation pathway of phenol through the ortho-pathway. The Q-PCR results showed that the transcripts of both the phenol hydroxylase gene and catechol 1,2-dioxygenase gene were up-regulated under the stimulation of phenol, demonstrating again that the strain degraded phenol via ortho-degradation pathway.

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“…Genomic sequencing of Sulfolobus solfataricus st. P2 found genes for aromatic degradation and it was found to be able to degrade phenol aerobically through meta -ring cleavage [ 58 ]. A strain of the closely related thermophilic Sulfolobus solfataricus st. 98/2 was later found to be able to degrade phenol at 80°C and 3.2 pH [ 59 , 60 ] through meta -ring cleavage also [ 61 ]. A dienelactone hydrolase from Sulfolobus solfataricus st. P1 was also identified and characterized [ 62 ].…”

Section: Degradation Of Organics With Thermophilic Sulfomentioning

confidence: 99%

Diversity and Niche of Archaea in Bioremediation

Krzmarzick

Taylor

Xiang

et al. 2018

Archaea

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Bioremediation is the use of microorganisms for the degradation or removal of contaminants. Most bioremediation research has focused on processes performed by the domain Bacteria; however, Archaea are known to play important roles in many situations. In extreme conditions, such as halophilic or acidophilic environments, Archaea are well suited for bioremediation. In other conditions, Archaea collaboratively work alongside Bacteria during biodegradation. In this review, the various roles that Archaea have in bioremediation is covered, including halophilic hydrocarbon degradation, acidophilic hydrocarbon degradation, hydrocarbon degradation in nonextreme environments such as soils and oceans, metal remediation, acid mine drainage, and dehalogenation. Research needs are addressed in these areas. Beyond bioremediation, these processes are important for wastewater treatment (particularly industrial wastewater treatment) and help in the understanding of the natural microbial ecology of several Archaea genera.

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Biochemical, Transcriptional and Translational Evidences of the Phenol-meta-Degradation Pathway by the Hyperthermophilic Sulfolobus solfataricus 98/2

Cited by 19 publications

References 40 publications

Biodegradation of phenol by free and immobilized Candida tropicalis NPD1401

Biodegradation of phenol by free and immobilized Candida tropicalis NPD1401

Degradation of phenol via ortho-pathway by Kocuria sp. strain TIBETAN4 isolated from the soils around Qinghai Lake in China

Diversity and Niche of Archaea in Bioremediation

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