Biochemische Studien über die Gifte der Schlangengattung Bothrops

Klobusitzky, D. Von; König, Peter

doi:10.1007/bf01924816

Cited by 49 publications

(2 citation statements)

References 4 publications

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“…Some serine proteases from South American Bothrops snakes were named Batroxobin, with the first being described as early as 1939 [33]. These enzymes have been widely used in the pharmaceutical industry, as defibrinogenating drugs or as reagents for the diagnosis of dysfibrinogenemias.…”

Section: Discussionmentioning

confidence: 99%

New Insights on Moojase, a Thrombin-Like Serine Protease from Bothrops moojeni Snake Venom

Amorim

Menaldo

Carone

et al. 2018

Toxins

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Snake venom serine proteases (SVSPs) are enzymes that are capable of interfering in various parts of the blood coagulation cascade, which makes them interesting candidates for the development of new therapeutic drugs. Herein, we isolated and characterized Moojase, a potent coagulant enzyme from Bothrops moojeni snake venom. The toxin was isolated from the crude venom using a two-step chromatographic procedure. Moojase is a glycoprotein with N-linked glycans, molecular mass of 30.3 kDa and acidic character (pI 5.80–6.88). Sequencing of Moojase indicated that it is an isoform of Batroxobin. Moojase was able to clot platelet-poor plasma and fibrinogen solutions in a dose-dependent manner, indicating thrombin-like properties. Moojase also rapidly induced the proteolysis of the Aα chains of human fibrinogen, followed by the degradation of the Bβ chains after extended periods of incubation, and these effects were inhibited by PMSF, SDS and DTT, but not by benzamidine or EDTA. RP-HPLC analysis of its fibrinogenolysis confirmed the main generation of fibrinopeptide A. Moojase also induced the fibrinolysis of fibrin clots formed in vitro, and the aggregation of washed platelets, as well as significant amidolytic activity on substrates for thrombin, plasma kallikrein, factor Xia, and factor XIIa. Furthermore, thermofluor analyses and the esterase activity of Moojase demonstrated its very high stability at different pH buffers and temperatures. Thus, studies such as this for Moojase should increase knowledge on SVSPs, allowing their bioprospection as valuable prototypes in the development of new drugs, or as biotechnological tools.

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Section: Discussionmentioning

confidence: 99%

New Insights on Moojase, a Thrombin-Like Serine Protease from Bothrops moojeni Snake Venom

Amorim

Menaldo

Carone

et al. 2018

Toxins

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“…Paper Reactivity Hosoya et al (1951); Kutzim (1952a Visual inspection and dyes von Klobusitzky and Konig (1939) separation of yellow pigment from snake venom; Grassmann (1951) fractionation of five protein-like components in snake toxin Animal toxins ,, Activity Wieland, Wirth, and Fischer (1949); Neumann and Habermann (1952) It is within the capacity of any laboratory to use and maintain simple reversible electrodes. By proper planning of work, time can be allotted in any laboratory for buffer equilibriums to be established before protein boundaries are made and the run started.…”

Section: Antibioticsmentioning

confidence: 99%

The Use and Limitations of Filter-paper Electrophoresis

Martin¹,

Franglen²

1954

Journal of Clinical Pathology

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In 1935 Tiselius, using the apparatus he had devised in 1930, demonstrated that human serum globulin fell into three reasonably defined groups based on their electrophoretic mobilities. For the next 15 years a steady flood of papers reported the fluctuations of albumin and the globulins in young and old, in health and disease, as disclosed by analyses carried out using Tiselius apparatus with and without the numerous minor modifications which were introduced.In spite of brilliant efforts at simplification it was evident that the method, because of its relative complexity, could never have widespread use in routine clinical chemistry. In 1950 three groups of workers, among them Tiselius himself, described methods which indicated that, using filter paper as a matrix, a relatively cheap and rapid method could be evolved of obtaining a rough analysis of the serum proteins akin to the analysis obtained by the classical technique.The method required no more than 0.5 ml. of serum, and though estimates of the total protein concentration would still have to be made by other means, it was conceivable that this simple technique would dispense with the tedious salt fractionation and subsequent analyses required heretofore to obtain the albumin/globulin ratio.The last four years have produced a spate of papers devoted to the description of modifications in methods of separation and analysis with numerous technical and mathematical tricks for improving the correlation of the results with the substantial body of data already obtained with the classical technique. With few notable exceptions scant attention was paid to the fundamental principles underlying the technique or to the points in which their application to it differed from the classical procedure.As paper succeeded paper it became increasingly evident that except within fairly wide limits the two methods were not quantitatively comparable.Even worse, because of lack of time spent on a consideration of the basic principles involved, G it seemed probable that differing techniques for the production of " electrophoretograms " were not comparable among themselves.One was faced with the prospect of scores of laboratories turning out hundreds of results year by year; each group, because of deviations in technique unappreciated and therefore unrecorded, differing to an unknown extent one from the other. The clinical chemical chaos that could arise demanded a reassessment of the situation in the hope that workers in the field would stop, think, and start again.Electrokinetic phenomena, as the derivation of the word implies, are concerned with the

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The Use of Serum Protein Electrophoresis in Clinical Medicine

Wall¹

1958

Arch Intern Med

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Biochemische Studien über die Gifte der Schlangengattung Bothrops

Cited by 49 publications

References 4 publications

New Insights on Moojase, a Thrombin-Like Serine Protease from Bothrops moojeni Snake Venom

New Insights on Moojase, a Thrombin-Like Serine Protease from Bothrops moojeni Snake Venom

The Use and Limitations of Filter-paper Electrophoresis

The Use of Serum Protein Electrophoresis in Clinical Medicine

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