Biochemistry and molecular biology of the anaerobic response

Dennis, E. S.; Olive, Mark R.; MILLAR, A. Harvey; Peacock, W. James; Setter, Tim L.; Wray, John L.

doi:10.1017/cbo9780511600395.012

Cited by 6 publications

(3 citation statements)

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“…Induction of ADH may occur as a result of such signals but is probably more likely to represent a classic response to a nominal 'anaerobic' state which exists for a sufficiently long time window [ 1,10,12,26,30]. ADH is one of a number of enzymes involved in glycolysis and fermentation which are induced in response to a low partial pressure of 02, i.e.…”

Section: Discussionmentioning

confidence: 99%

Rapid changes in oxidative metabolism as a consequence of elicitor treatment of suspension-cultured cells of French bean (Phaseolus vulgaris L.)

et al. 1995

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Stressed plant cells often show increased oxygen uptake which can manifest itself in the transient production of active oxygen species, the oxidative burst. There is a lack of information on the redox status of cells during the early stages of biotic stress. In this paper we measure oxygen uptake and the levels of redox intermediates NAD/NADH and ATP and show the transient induction of the marker enzyme for redox stress, alcohol dehydrogenase. Rapid changes in the redox potential of elicitor-treated suspension cultures of French bean cells indicate that, paradoxically, during the period of maximum oxygen uptake the levels of ATP and the NADH/NAD ratio fall in a way that indicates the occurrence of stress in oxidative metabolism. This period coincides with the maximum production of active oxygen species particularly H2O2. The cells recover and start producing ATP immediately of H2O2 production. This indicates that the increased O2 uptake is primarily incorporated into active O2 species. A second consequence of these changes is probably a transient compromising of the respiratory status of the cells as indicated in expression of alcohol dehydrogenase. Elicitor-induced bean ADH was purified to homogeneity and the M(r) 40,000 polypeptide was subjected to amino acid sequencing. 15% of the whole protein was sequenced from three peptides and was found to have nearly 100% sequence similarity to the amino acid sequence for pea ADH1 (PSADH1). The cDNA coding for the pea enzyme was used to demonstrate the transient induction of ADH mRNA in elicitor-treated bean cells. Enzyme activity levels also increased transiently subsequently. Increased oxygen uptake has previously been thought to be associated with provision of energy for the changes in biosynthesis that occur rapidly after perception of the stress signal. However the present work shows that this rapid increase in oxygen uptake as a consequence of elicitor action is not wholly associated with respiration.

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Section: Discussionmentioning

confidence: 99%

Rapid changes in oxidative metabolism as a consequence of elicitor treatment of suspension-cultured cells of French bean (Phaseolus vulgaris L.)

et al. 1995

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“…glycet'aldehyde-.3-phosphutc dehydrogenase and sucrose synthase (Bailey-SetTes et ak 1988. Dennis et al 1992. Hake et al 1985.…”

Section: Introductionmentioning

confidence: 99%

Purification and differential expression of enolase from maize

Lal¹,

Kelley²,

Elthon³

1994

Physiol Plant

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“…A partir do piruvato são produzidos os principais produtos da fermentação em tecidos vegetais: etanol, lactato e alanina. Embora a atividade conjunta da glicólise e fermentação permitam apenas a síntese limitada de ATP, pela fosforilação ao nível de substrato, regenera-se NAD + e remove-se o excesso de prótons (Dennis et al, 1992;Armstrong et al, 1994, Ricard et al, 1994Morard & Silvestre, 1996, Drew, 1997Sousa & Sodek, 2002). A injúria e morte das raízes tem sido atribuída ao acúmulo de metabólitos tóxicos produzidos durante a fermentação (principalmente etanol), diminuição no metabolismo energético e a falta de substratos para a respiração (Drew, 1997).…”

Section: Fixação Simbiótica De N 2 E Alterações Metabólicas Em Planta...unclassified

Modificações morfologicas e assimilação de nitrogenio em plantas de soja (Glycine max) com sistemas radiculares sob deficiencia de O2

Thomas¹,

Sodek²

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Biochemistry and molecular biology of the anaerobic response

Cited by 6 publications

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Rapid changes in oxidative metabolism as a consequence of elicitor treatment of suspension-cultured cells of French bean (Phaseolus vulgaris L.)

Rapid changes in oxidative metabolism as a consequence of elicitor treatment of suspension-cultured cells of French bean (Phaseolus vulgaris L.)

Purification and differential expression of enolase from maize

Modificações morfologicas e assimilação de nitrogenio em plantas de soja (Glycine max) com sistemas radiculares sob deficiencia de O2

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