Biochemistry and physiology of zebrafish photoreceptors

Zang, Jingjing

doi:10.1007/s00424-021-02528-z

Cited by 32 publications

(25 citation statements)

References 219 publications

(244 reference statements)

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“…The adult UV cone-enriched paralogs are all involved in calcium-mediated feedback regulation of the phototransduction shut-off cascade and recovery/adaptation in cones (Fig. 3 ) 8 , 14 , 50 , 64 . Future functional studies will be required to determine the precise functional role of this adult UV cone-enriched gene expression program.…”

Section: Discussionmentioning

confidence: 99%

“…Teleost fishes occupy a wide diversity of aquatic habitats and have expanded their opsin repertoires to adapt to these diverse photic niches. Zebrafish ( Danio rerio ) is widely used as a model system in photoreceptor research 14 , 15 . While the zebrafish genome encodes a single UV cone opsin gene ( opn1sw1 ) and a single blue cone opsin gene ( opn1sw2 ), it contains a syntenic array of four green cone opsins tuned to a range of wavelengths ( opn1mw1 , λ max = 467 nm; opn1mw2, λ max = 476 nm; opn1mw3, λ max = 488 nm and opn1mw4, λ max = 505 nm), as well as a tandem array of two red cone opsin genes ( opn1lw1 , λ max = 558 nm; opn1lw2, λ max = 548 nm) 16 .…”

Section: Introductionmentioning

confidence: 99%

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Partitioning of gene expression among zebrafish photoreceptor subtypes

Ogawa

Corbo

2021

Sci Rep

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Vertebrate photoreceptors are categorized into two broad classes, rods and cones, responsible for dim- and bright-light vision, respectively. While many molecular features that distinguish rods and cones are known, gene expression differences among cone subtypes remain poorly understood. Teleost fishes are renowned for the diversity of their photoreceptor systems. Here, we used single-cell RNA-seq to profile adult photoreceptors in zebrafish, a teleost. We found that in addition to the four canonical zebrafish cone types, there exist subpopulations of green and red cones (previously shown to be located in the ventral retina) that express red-shifted opsin paralogs (opn1mw4 or opn1lw1) as well as a unique combination of cone phototransduction genes. Furthermore, the expression of many paralogous phototransduction genes is partitioned among cone subtypes, analogous to the partitioning of the phototransduction paralogs between rods and cones seen across vertebrates. The partitioned cone-gene pairs arose via the teleost-specific whole-genome duplication or later clade-specific gene duplications. We also discovered that cone subtypes express distinct transcriptional regulators, including many factors not previously implicated in photoreceptor development or differentiation. Overall, our work suggests that partitioning of paralogous gene expression via the action of differentially expressed transcriptional regulators enables diversification of cone subtypes in teleosts.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Partitioning of gene expression among zebrafish photoreceptor subtypes

Ogawa

Corbo

2021

Sci Rep

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“…Animals were fasted overnight to eliminate confounding effects of variable feeding behavior on glucose measurements. They were then dark adapted for two hours, which contracts pigments to the basal RPE, in order to minimize pigment granule interference with visualization of photoreceptors [33]. The animals were then euthanised on ice and glucose was measured.…”

Section: Histologymentioning

confidence: 99%

Differential Responses of Neural Retina Progenitor Populations to Chronic Hyperglycemia

Schmitner¹,

Recheis²,

Thönig³

et al. 2021

Cells

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Diabetic retinopathy is a frequent complication of longstanding diabetes, which comprises a complex interplay of microvascular abnormalities and neurodegeneration. Zebrafish harboring a homozygous mutation in the pancreatic transcription factor pdx1 display a diabetic phenotype with survival into adulthood, and are therefore uniquely suitable among zebrafish models for studying pathologies associated with persistent diabetic conditions. We have previously shown that, starting at three months of age, pdx1 mutants exhibit not only vascular but also neuro-retinal pathologies manifesting as photoreceptor dysfunction and loss, similar to human diabetic retinopathy. Here, we further characterize injury and regenerative responses and examine the effects on progenitor cell populations. Consistent with a negative impact of hyperglycemia on neurogenesis, stem cells of the ciliary marginal zone show an exacerbation of aging-related proliferative decline. In contrast to the robust Müller glial cell proliferation seen following acute retinal injury, the pdx1 mutant shows replenishment of both rod and cone photoreceptors from slow-cycling, neurod-expressing progenitors which first accumulate in the inner nuclear layer. Overall, we demonstrate a diabetic retinopathy model which shows pathological features of the human disease evolving alongside an ongoing restorative process that replaces lost photoreceptors, at the same time suggesting an unappreciated phenotypic continuum between multipotent and photoreceptor-committed progenitors.

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“…The photoreceptor cells contain two types, namely cones and rods. Cones are cone cells responsible for bright vision and color differential, while rods are rod cells responsible for low vision and bright level information provider (Vinberg et al, 2018;Zang & Neuhauss, 2021). Several freshwater fish species are included in a blue-sensitive cone that only have one cone cell with dominated rod cells (Nabiu et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

The effects of LED light spectrum manipulation on growth and color performance of giant gourami Osphronemus gouramy Lacepede Padang strain

Gunawan

Nirmala

Soelistyowati

et al. 2022

Jurnal Akuakultur Indonesia

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This study aimed to evaluate the effect of light spectrum on growth and color performance of giant giant gourami Padang strain. The experiment used a completely randomized design (RAL) with four light emitting diode (LED) treatments in different emission spectra (white, red, green, and blue) at 550 Lux intensity, compared to the control treatment (light room with white tubular lamp at 50 Lux intensity). The irradiation was carried out for 12 hours of photoperiod. The fish used had the total length of 82.90±4.2 mm and body weight of 9.87 ± 0.99 g. The highest growth performance was found in blue LED treatment with the specific growth rate of 2.73 ± 0.2% and feed efficiency of 86.26 ± 2.71%. The best color performance was found in red LED treatment with the RGB ratio of 44.57 ± 0.62% in dorsal fin, 38.41 ± 1.36% in pectoral fins, and 45.33 ± 2.25% in anal fin with the chromatophore cell concentration at 1.973±58 cells/mm2. Keywords : Osphronemus gouramy, blue LED, spectrum, chromatophore, light ABSTRAK Penelitian ini bertujuan untuk mengevaluasi pengaruh spektrum cahaya terhadap kinerja pertumbuhan dan warna ikan gurami strain Padang. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengan 4 perlakuan cahaya lampu light emitting diodes (LED) yang memiliki spektrum panjang gelombang berbeda (putih, merah, hijau dan biru) intensitas 550 Lux dan kontrol (cahaya ruang berasal dari lampu tubular putih intensitas 50 Lux). Penyinaran dilakukan selama 12 jam mengikuti fotoperiod. Ikan uji yang digunakan memiliki panjang total 82,90 ± 4,2 mm, dengan bobot 9,87 ± 0,99 g. Kinerja pertumbuhan terbaik terdapat pada perlakuan LED biru dengan laju pertumbuhan spesifik sebesar 2,73 ± 0,2% dan efisiensi pakan sebesar 86,26 ± 2,71%. Performa warna terbaik terdapat pada perlakuan LED merah dengan rasio warna merah pada RGB bagian dorsal sebesar 44,57 ± 0,62%, sirip pektoral sebesar 38,41 ± 1,36%, dan sirip anal sebesar 45,33 ± 2,25% dengan jumlah sel kromatofor sebanyak 1973 sel/mm2. Kata kunci : Osphronemus gouramy, LED biru, spektrum, kromatofor, cahaya

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Biochemistry and physiology of zebrafish photoreceptors

Cited by 32 publications

References 219 publications

Partitioning of gene expression among zebrafish photoreceptor subtypes

Partitioning of gene expression among zebrafish photoreceptor subtypes

Differential Responses of Neural Retina Progenitor Populations to Chronic Hyperglycemia

The effects of LED light spectrum manipulation on growth and color performance of giant gourami Osphronemus gouramy Lacepede Padang strain

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