Biochemistry of Bakanae Fungus. Part VII

Yabuta, T.; Sumiki, Yusuke; Katayama, Eisaku; Motoyama, Hiroshi

doi:10.1271/nogeikagaku1924.16.11_1157

Cited by 7 publications

(7 citation statements)

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“…fujikuroi is well known for the production of the plant hormone gibberellic acid (GA), the causative agent of bakanae disease on rice, for more than 100 years (25). Besides gibberellins, the fungus produces a wide range of other secondary metabolites, such as the red polyketide pigments bikaverin (26,27) and fusarubins (28,29) and mycotoxins such as fusarin C (30), fusaric acid (31), or fumonisins (32).…”

mentioning

confidence: 99%

Two Histone Deacetylases, FfHda1 and FfHda2, Are Important for Fusarium fujikuroi Secondary Metabolism and Virulence

Studt

Schmidt

Jahn

et al. 2013

Appl Environ Microbiol

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Histone modifications are crucial for the regulation of secondary metabolism in various filamentous fungi. Here we studied the involvement of histone deacetylases (HDACs) in secondary metabolism in the phytopathogenic fungus Fusarium fujikuroi, a known producer of several secondary metabolites, including phytohormones, pigments, and mycotoxins. Deletion of three Zn 2؉ -dependent HDAC-encoding genes, ffhda1, ffhda2, and ffhda4, indicated that FfHda1 and FfHda2 regulate secondary metabolism, whereas FfHda4 is involved in developmental processes but is dispensable for secondary-metabolite production in F. fujikuroi. Single deletions of ffhda1 and ffhda2 resulted not only in an increase or decrease but also in derepression of metabolite biosynthesis under normally repressing conditions. Moreover, double deletion of both the ffhda1 and ffhda2 genes showed additive but also distinct phenotypes with regard to secondary-metabolite biosynthesis, and both genes are required for gibberellic acid (GA)-induced bakanae disease on the preferred host plant rice, as ⌬ffhda1 ⌬ffhda2 mutants resemble the uninfected control plant. Microarray analysis with a ⌬ffhda1 mutant that has lost the major HDAC revealed differential expression of secondarymetabolite gene clusters, which was subsequently verified by a combination of chemical and biological approaches. These results indicate that HDACs are involved not only in gene silencing but also in the activation of some genes. Chromatin immunoprecipitation with the ⌬ffhda1 mutant revealed significant alterations in the acetylation state of secondary-metabolite gene clusters compared to the wild type, thereby providing insights into the regulatory mechanism at the chromatin level. Altogether, manipulation of HDAC-encoding genes constitutes a powerful tool to control secondary metabolism in filamentous fungi.

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mentioning

confidence: 99%

Two Histone Deacetylases, FfHda1 and FfHda2, Are Important for Fusarium fujikuroi Secondary Metabolism and Virulence

Studt

Schmidt

Jahn

et al. 2013

Appl Environ Microbiol

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“…lycopersici에서 분리한 대사산물 중 하나라고 하였다 (Gaumann, 1957;Yabuta, 1937). 또한 Bacon et al(1996) (Davis, 1969;Luz et al, 1990;Marasas et al, 1984;Porter et al, 1990;Smith and Sousadias, 1993) (Greenberg and Yao, 2004;Kim et al, 1998;Kuzniak, 2001).…”

Section: Fusaric Acid는 Yabuta Et Al(1937)에 의하여 처음 보고되unclassified

Role of a Phytotoxin Produced by Fusarium oxysporum f. sp. raphani on Pathogenesis of and Resistance to the Fungus

Shim¹,

Kim²,

Jang³

et al. 2013

Korean Journal of Horticultural Science and Technology

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Abstract. In the course of a developing screening method for resistant radish to Fusarium oxysporum f. sp. raphani, we found that the fungus produces phytotoxic compound against Raphanus sativus. The culture filtrate of F. oxysporum f. sp. raphani KR1 represented the strongest phytotoxicity when the fungus was incubated in the malt extract broth with 150 rpm at 25°C for 14 days. Under bioassay-guided purification, we isolated a substance from liquid culture of F. oxysporum f. sp. raphani KR1, with phytotoxic effect against R. sativus. The compound was identified as fusaric acid by mass and nuclear magnetic resonance spectral analyses. Phytotoxicity of the compound against cruciferous vegetable crops, including radish, cabbage, and broccoli, was investigated. Fusaric acid represented phytotoxicity on radish seedlings by concentration dependant manner. And the phytotoxin demonstrated strong phytotoxicity on the resistant cultivars as well as susceptible cultivars of radish to F. oxysporum f. sp. raphani. In addition, fusaric acid isolated from the fungus also showed a potent phytotoxic efficacy against non-host Brassicaceae crops of the fungus such as cabbage and broccoli. The results demonstrate that fusaric acid produced by F. oxysporum f. sp. raphani is non-host-specific toxin and for screening of resistant radish to the fungal pathogen, spore suspension of the fungus without the phytotoxin has to be used.

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“…Infected plants would grow excessively taller than normal healthy plants, and being fall over and be unharvestable. This disease was found to be caused by a fungus known as Gibberella fujikuroi (Yabuta, T., 1935). Thus, Work on this special disease in Japan occurred by Japanese scientists who isolated a growth promoting substances from cultures of a fungus that parasitizes rice plants in the 1930s.…”

Section: Biosynthesis Of Gibberellinsmentioning

confidence: 99%

Terpenoids and Gibberellic Acids Interaction in Plants

Asrar¹

2012

Advances in Selected Plant Physiology Aspects

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Biochemistry of Bakanae Fungus. Part VII

Cited by 7 publications

References 0 publications

Two Histone Deacetylases, FfHda1 and FfHda2, Are Important for Fusarium fujikuroi Secondary Metabolism and Virulence

Two Histone Deacetylases, FfHda1 and FfHda2, Are Important for Fusarium fujikuroi Secondary Metabolism and Virulence

Role of a Phytotoxin Produced by Fusarium oxysporum f. sp. raphani on Pathogenesis of and Resistance to the Fungus

Terpenoids and Gibberellic Acids Interaction in Plants

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