Biochemistry of insecticide resistance

Oppenoorth, F. J.

doi:10.1016/0048-3575(84)90088-9

Cited by 169 publications

(166 citation statements)

References 27 publications

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“…catalyse the hydrolysis of a wide range of xenobiotic carboxyesters and aromatic amides and is involved in a major route for the detoxification and the activation of such compounds (Krueger et al, 1953;Hosokawa et al, 1987Hosokawa et al, , 1990Kett erman et al, 1992) and subsequently in insecticide resistance (Oppenoorth, 1985).…”

Section: Discussionmentioning

confidence: 99%

Purification and characterization of a carboxylesterase involved in malathion-specific resistance from Tribolium castaneum (Coleoptera: Tenebrionidae)

Haubruge

Amichot²,

Cuany³

et al. 2002

Insect Biochemistry and Molecular Biology

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Specific resistance to malathion in a strain of Tribolium castaneum is due to a 44-fold increase in malathion carboxylesterase (MCE) activity relative to a susceptible strain, whereas non-specific esterase levels are slightly lower. Unlike the overproduced esterase of some mosquito and aphid species, MCE in Tribolium castaneum accounts for only a small fraction (0.033-0.045%) of the total extractable protein respectively in resistant and susceptible strains. The enzyme was purified to apparent homogeneity from these two strains and has a similar molecular weight of 62,000. However, preparative isoelectricfocusing indicated that resistant insects possess one MCE with pI of 7.3, while susceptible insects possess a MCE with a pI of 6.6. Purified MCE from both populations had different K m and V m values for hydrolysis of malathion as well as for α-naphthyl acetate. The kinetic analysis suggests that MCE of resistant insects hydrolyses malathion faster than the purified carboxylesterase from susceptible beetles and that this enzyme has greater affinity for malathion than for naphthyl esters. Malathion-specific resistance is due to the presence of a qualitatively different esterase in the resistant strain.

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Section: Discussionmentioning

confidence: 99%

Purification and characterization of a carboxylesterase involved in malathion-specific resistance from Tribolium castaneum (Coleoptera: Tenebrionidae)

Haubruge

Amichot²,

Cuany³

et al. 2002

Insect Biochemistry and Molecular Biology

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“…The resistance level to deltamethrin in the highest resistant sample (Salta), was similar to those reported for Brazilian T. infestans (RR 7.0) and Venezuelan R. prolixus (RR 11.4). Resistance to pyrethroids in insects has been correlated with enhanced metabolism by enzymes (Oppenoorth 1985, Wilkinson 1983, and particularly a higher cytochrome P450s activity was measured in pyrethroid resistance in housefly (Lee & Scott 1989). Hung & Sun (1989), using ethoxycoumarine as substrate, found that larvae homogenates of fenvalerate resistant diamond moth, possessed 100 fold higher activity than susceptible insects.…”

Section: Discussionmentioning

confidence: 99%

Role of enhanced detoxication in a deltamethrin-resistant population of Triatoma infestans (Hemiptera, Reduviidae) from Argentina

Audino

Vassena

Barrios

et al. 2004

Mem. Inst. Oswaldo Cruz

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“…This shows that DEF enhanced synergism in acephate, fenvalerate and endosulfan. Pyrethroid resistance has been attributed to reduced neural sensitivity, enhanced metabolism and reduced penetration ratio in many insects [Oppenoorth, 1985;Zerba et al, 1987). Atkinson et al (1991) reported that permethrin and cypermethrin resistance in a highly pyrethroid resistant strain of Blattella germanica was partially suppressed with PBO and DEF, thus suggesting the involvement of enhanced metabolism as well as target site insensitivity in the mechanism of resistance.…”

Section: Synergism Studiesmentioning

confidence: 99%

Detection of insecticide resistance and mechanisms of resistance in field populations of Chrysoperla zastrowi sillemi (Neuroptera: Chrysopidae) collected from different geographical locations in India

Venkatesan

Mahiba²,

Jalali

et al. 2018

Journal of Biological Control

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ABSTRACT:The toxic effect of commonly used insecticides in cotton fields was studied on 9 populations of Chrysoperla zastrowi sillemi (Esben-Petersen), an important predator of sucking pests collected in India. The dose mortality bioassay against 3-days old larvae was determined using three insecticides viz., endosulfan, fenvalerate and acephate by topical bioassay method. Mechanism of resistance to the above mentioned insecticides were determined without and with three metabolic inhibitors (synergists), viz., piperonyl butoxide (PBO), S,S,S-tributyl-phosphorotrithioate (DEF) and diethyl maleate (DEM). Among the populations, resistant ratios (RR) of CZS-8 was significantly higher i.e. 50.36., 66.11 and 277.51-fold for endosulfan, fenvalerate and acephate, respectively compared to susceptible population (CZS-10). The CZS-8 was selected for synergism study it showed higher LC 50 values and resistance ratio for all three insecticides. It showed 8.97-fold, 18.49-fold and 6.38-fold increase in synergism ratio for endosulfan indicating the resistance was strongly synergised by PBO, DEF and DEM. Similarly for fenvalerate, CZS-8 showed 8.69-fold and 3.63-fold significant increase in synergism ratio by DEF and DEM, respectively and for acephate, CZS-8 showed 54.82-fold, 150.87-fold and 113.52-fold significant increase in synergism ratio indicating that the resistance could be due to cytochrome p-450, esterase and glutathione s-transferase activity. The study indicated that the field population of C. z. sillemi developed resistance to different groups of insecticides. Among different geographical populations, CZS-8 collected from Sriganganagar, was recorded as most resistant.

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Biochemistry of insecticide resistance

Cited by 169 publications

References 27 publications

Purification and characterization of a carboxylesterase involved in malathion-specific resistance from Tribolium castaneum (Coleoptera: Tenebrionidae)

Purification and characterization of a carboxylesterase involved in malathion-specific resistance from Tribolium castaneum (Coleoptera: Tenebrionidae)

Role of enhanced detoxication in a deltamethrin-resistant population of Triatoma infestans (Hemiptera, Reduviidae) from Argentina

Detection of insecticide resistance and mechanisms of resistance in field populations of Chrysoperla zastrowi sillemi (Neuroptera: Chrysopidae) collected from different geographical locations in India

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