Biochemistry of the effects of asiaticoside on human polymorphonuclear leukocytes

Karan, A; Ozand, P

doi:10.1016/0006-2952(72)90025-1

Cited by 1 publication

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, asiaticoside signaling may involve the TGF‐β signaling pathway, as mentioned previously. Second, asiaticoside may mimic the effect of steroid hormones (Karan and Ozand, ). In this regard, the pentacyclic triterpene structure of asiaticoside is similar to that of steroid‐like compounds; thus, it can diffuse through the target cell membrane and further regulate cell behavior.…”

Section: Discussionmentioning

confidence: 99%

Asiaticoside Induces Type I Collagen Synthesis and Osteogenic Differentiation in Human Periodontal Ligament Cells

Nowwarote

Osathanon

Jitjaturunt

et al. 2012

Phytotherapy Research

View full text Add to dashboard Cite

Asiaticoside, an active ingredient extracted from Centella asiatica, has been widely used to promote wound healing. In this study, the effects of asiaticoside on proliferation, protein synthesis, and osteogenic differentiation in human periodontal ligament cells (HPDLs) were investigated. HPDLs were treated with asiaticoside at concentrations of 25, 50, and 100 µg/mL. Cell number was determined by MTT assay. The mRNA expression was analyzed by reverse transcription-polymerase chain reaction. Western blot analysis and immunocytochemistry were used to confirm protein synthesis. Osteogenic differentiation was determined by alkaline phosphatase activity, osteoblast marker gene expression, and in vitro mineralization. The results showed that asiaticoside treatment, ranging from 25 to 100 mg/mL, had no effect on cytotoxicity or cell proliferation. When HPDLs were treated with asiaticoside in serum-free medium, dose-dependent increases in the levels of fibronectin and collagen type I mRNA and protein were observed at 72 h. Moreover, asiaticoside attenuated matrix metalloproteinase-1 but enhanced tissue inhibitor of metalloproteinase-1 mRNA expression. The addition of asiaticoside to osteogenic medium resulted in an increase in alkaline phosphatase enzymatic activity, up-regulation of osteoblast marker gene mRNA expression, and enhancement of mineralization by HPDLs. These results suggest the potential application of asiaticoside for enhancing periodontal tissue healing.

show abstract

Section: Discussionmentioning

confidence: 99%