Biocidal effects of volatile organic compounds produced by the myxobacterium Corrallococcus sp. EGB against fungal phytopathogens

Ye, Xianfeng; Yang, Chen; Ma, Shiyun; Yuan, Tian; Wu, Y. C.; Li, Yingxuan; Zhao, Yuqiang; Chen, Shuying; Zhang, Yiwen; Li, Liuyan; Li, Zhoukun; Huang, Yan; Cao, Hui; Cui, Zhongli

doi:10.1016/j.fm.2020.103502

Cited by 36 publications

(23 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A dual culture method was used to evaluate the inhibitory effect of antagonistic bacteria produced VOCs on A. iridiaustralis mycelial growth and conidial germination ( Ye et al, 2020 ; Di Francesco et al, 2020b ). SDW was used to inoculate the control plates with the same amounts of bacteria as the test plates, which was 80 μl of bacterial suspension at a concentration of 10 6 –10 9 colony forming units (CFU)/ml.…”

Section: Methodsmentioning

confidence: 99%

Volatile organic compounds produced by Bacillus velezensis L1 as a potential biocontrol agent against postharvest diseases of wolfberry

et al. 2022

View full text Add to dashboard Cite

Volatile organic compounds (VOCs) produced by antagonistic microorganisms have good biocontrol prospects against postharvest diseases. Infection caused by Alternaria iridiaustralis and 10 other significant fungal diseases can be successfully inhibited by VOCs produced by an identified and screened endophytic strain L1 (Bacillus velezensis). This study revealed the in vivo and in vitro biocontrol effects of VOCs released by B. velezensis L1 on A. iridiaustralis, a pathogenic fungus responsible for rot of wolfberry fruit. The inhibition rates of VOCs of B. velezensis L1 on the mycelial growth of A. iridiaustralis in vitro were 92.86 and 90.30%, respectively, when the initial inoculum concentration on the plate was 1 × 109 colony forming unit (CFU)/ml. Spore germination and sporulation were 66.89 and 87.96%, respectively. VOCs considerably decreased the wolfberry’s disease index and decay incidence in vivo. Scanning electron microscopy revealed that the morphological and structural characteristics of A. iridiaustralis could be altered by VOCs. Ten VOCs were identified through headspace-gas chromatography-ion mobility spectrometry. Pure chemical tests revealed that 2.3-butanedione had the strongest antifungal effects, totally inhibiting A. iridiaustralis in wolfberry fruit at a 60 μl/L concentration. The theory underpinning the potential application of VOCs from B. velezensis is provided herein. This is also the first study to document the antifungal capabilities of the B. velezensis strain on postharvest wolfberry fruit.GRAPHICAL ABSTRACT

show abstract

Section: Methodsmentioning

confidence: 99%

Volatile organic compounds produced by Bacillus velezensis L1 as a potential biocontrol agent against postharvest diseases of wolfberry

et al. 2022

View full text Add to dashboard Cite

show abstract

“…EGB. At a concentration of 114.2 μl L −1 , it could control postharvest blue mold disease caused by Penicillium digitatum in citrus and reduce the lesion area by 53% ( Ye et al, 2020 ). After fumigation of mango with 2-Ethyl-5-Methylpyrazine, a VOC of strain Pseudomonas putida BP25, for 24 h, the severity of anthracnose on fruits decreased by >76% compared with the control ( Archana et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…For instance, Isooctanol produced by Corallococcus sp. exhibits antifungal activity in Fusarium oxysporum ( Ye et al, 2020 ). 3-Methylbutan-1-ol has suppressed Botrytis cinerea growth and infection on tomato fruit at 0.442 ml L −1 ( Chaouachi et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity of the volatile organic compounds produced by Ceratocystis fimbriata strains WSJK-1 and Mby

Gao

Ren²,

ShuQi³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Microorganism-produced volatile organic compounds (VOCs) are considered promising environmental-safety fumigants in food preservation. In this study, the VOCs from fungal Ceratocystis fimbriata strains (WSJK-1, Mby) were tested against postharvest fungi Monilinia laxa, Fusarium oxysporum, Monilinia fructicola, Botrytis cinerea, Alternaria solani, and Aspergillus flavus in vitro. The mycelial growth was significantly inhibited, in particular M. fructicola and B. cinerea (76.95, 76.00%), respectively. VOCs were identified by headspace solid-phase microextraction coupled with Gas Chromatography–Mass Spectrometry (HS-SPME-GC–MS); 40 compounds were identified. The antifungal activity of 21 compounds was tested by the minimum inhibitory concentrations (MIC) value. Benzaldehyde, 2-Phenylethanol, and 1-Octen-3-ol showed strong antifungal activity with the MIC in vitro ranging from 0.094 to 0.284 ml L−1 depending on the pathogen tested. The optical microscope showed serious morphological damage, including cell deformation, curling, collapse, and deficiency in mycelial or conidia cell structures treated with C. fimbriata VOCs and pure compounds. In vivo tests, C. fimbriata VOCs decreased brown rot severity in peaches, and compounds Benzaldehyde and 2-Phenylethanol could reduce peach brown rot in peaches at 60 μl L−1. The VOCs produced by C. fimbriata strain have good antifungal effects; low concentration fumigation could control peach brown rot. Its fragrance is fresh, safe, and harmless, and it is possible to replace chemical fumigants. It could be used as a potential biofumigant to control fruit postharvest transportation, storage, and food preservation. To the best of our knowledge, this is the first report on the antifungal activity and biocontrol mechanism of VOCs produced by C. fimbriata.

show abstract

“…Many biological control agents (BCAs), such as Pseudomonas , Streptomyces , Bacillus and Myxobacteria, have been used for disease control. The antagonisms of microorganisms against phytopathogenic fungi are mainly dependent on their secretion of active antimicrobial compounds (Kurniawan et al, 2018), competition for nutrients (Eljounaidi et al, 2016), induced systemic resistance (Arriel‐Elias et al, 2019), productions of cell wall degrading enzymes (Li et al, 2019) and volatile organic compounds (Ye et al, 2020). Currently, Burkholderia is a common gram‐negative bacterium that is typically isolated from soil, water and plant tissues, which has been regarded as a member of the growing list of BCAs (Ahmad et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol potential of Burkholderia sp. BV6 against the rice blast fungus Magnaporthe oryzae

Luo

Yang

Wang

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aim:To investigate the broad-spectrum antifungal activity of Burkholderia sp. BV6, that is isolated from rice roots and its biocontrol potential against rice blast caused by Magnaporthe oryzae. Methods and Results:We evaluated the ability to isolate BV6 in the biological control of rice blast disease and investigated its antifungal mechanisms. BV6 strongly inhibited the hyphal growth of M. oryzae Guy11 and other plant pathogenic fungi, and pot experiments showed that BV6 significantly decreases the disease index of rice blast from 47.5 to 24.6. The secreted small-molecule secondary metabolites were regarded as weapons during the antifungal process by inhibiting the germination of M. oryzae conidia and mycelial growth, and thereby prevent the following infection. Liquid chromatography-mass spectrometry analysis of the metabolites from the supernatant of isolate BV6 showed that the antifungal weapons of isolate BV6 are novel, small, molecular hydrophilic compounds that are different from reported antifungal compounds. Conclusions:The isolate BV6 inhibits the M. oryzae infection by the production of small-molecule antifungal compounds. Significance and Impact of the Study:The current study discovers the role of the Burkholderia sp. BV6 in the biocontrol of plant pathogenic fungi. Therefore, isolate BV6 is a potential candidate for developing a microbial formulation for the biocontrol of the most common disease of rice blast.

show abstract

Biocidal effects of volatile organic compounds produced by the myxobacterium Corrallococcus sp. EGB against fungal phytopathogens

Cited by 36 publications

References 49 publications

Volatile organic compounds produced by Bacillus velezensis L1 as a potential biocontrol agent against postharvest diseases of wolfberry

Volatile organic compounds produced by Bacillus velezensis L1 as a potential biocontrol agent against postharvest diseases of wolfberry

Antifungal activity of the volatile organic compounds produced by Ceratocystis fimbriata strains WSJK-1 and Mby

Biocontrol potential of Burkholderia sp. BV6 against the rice blast fungus Magnaporthe oryzae

Contact Info

Product

Resources

About