Biocompatibility and adhesion response of magnesium-hydroxyapatite/strontium-titania (Mg-HAp/ Sr-TiO<sub>2</sub>) bilayer coating on titanium

Praharaj, Rinmayee; Mishra, Snigdha; Misra, R.D.K.; Rautray, Tapash R.

doi:10.1080/10667857.2020.1825898

Cited by 23 publications

(4 citation statements)

References 40 publications

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“…MgHA-NCMCH-sSilk showed two distinct peaks in XRD patterns at 31.8° and 32.2°; these peaks are designated as ( 211) and (112), respectively. Broadening and overlap of peaks have revealed the semicrystalline nature of the HA structure [25,26]. The diffraction peaks are seen to be progressively changing to higher 2θ values upon closer inspection.…”

Section: Resultsmentioning

confidence: 99%

Magnesium-Substituted Hydroxyapatite /Spider Silk fibroin /N-Carboxymethyl Chitosan Based Microsphere: A potential template for bone regeneration

Swain,

Mishra,

Das

et al. 2024

Preprint

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Ageing, sedentary lifestyle and bone trauma have contributed to a sequel of clinical manifestation and risk factors associated with bone health. However, there are constant efforts made by researchers on both invasive and non-invasive strategies for a revival of bone architecture. In recent years, biomaterials, primarily biocomposites, have improved the facet of bone repair and regeneration. In the present work, a biocomposite of spider silk fibroin (SSF), N-caboxymethyl chitosan (N-CMCh) and magnesium (Mg2+) incorporated hydroxyapatite (HA), i.e. SSF/N-CMCh/Mg-HA was prepared to fabricate microspheres for bone regenerative applications. Initially, synthetic stoichiometric HA doped with Mg was synthesized by wet chemical method. The crystallographic property of Mg2+ incorporation in HA was investigated by XRD analysis. SEM-EDX and FTIR confirmed even distribution of the element and stoichiometric ratio of Ca:P along with Mg2+ in the biocomposite. A comparative analysis of the polarized SSF/N-CMCh/Mg-HA and SSF/N-CMCh was carried out to analyse the porosity and swelling of the fabricated microspheres. In vitro evaluation confirms the cellular metabolic viability of the microspheres. Further, Mg-HA-incorporated microspheres showed better protein absorption with enhanced ALP activity. Improved biocompatibility of the microspheres could be confirmed with high expression of osteogenic gene markers such as ALP, OCN, RUNX2, and COL-1. The studies confirmed that the fabricated SSF/N-CMCh/Mg-HA microspheres could be used as a potential template for bone regeneration.

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Section: Resultsmentioning

confidence: 99%

Magnesium-Substituted Hydroxyapatite /Spider Silk fibroin /N-Carboxymethyl Chitosan Based Microsphere: A potential template for bone regeneration

Swain,

Mishra,

Das

et al. 2024

Preprint

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“…Enhanced osseointegration should supply ample mechanical strength for the regeneration. During the resorption process, the mechanical strength of the scaffolds must be retained until the implantation area is totally replaced by the host tissues so that it can resume its structural role [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Mangiferin-Enriched Mn–Hydroxyapatite Coupled with β-TCP Scaffolds Simultaneously Exhibit Osteogenicity and Anti-Bacterial Efficacy

2023

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Biphasic calcium phosphate (BCP) containing β-tricalcium phosphate and manganese (Mn)-substituted hydroxyapatite (HAP) was synthesized. Biomedical scaffolds were prepared using this synthesized powder on a sacrificial polyurethane sponge template after the incorporation of mangiferin (MAN). Mn was substituted at a concentration of 5% and 10% in HAP to examine the efficacy of Mn at various concentrations. The phase analysis of the as-formed BCP scaffold was carried out by X-ray diffraction analysis, while the qualitative observation of morphology and the osteoblast cell differentiation were carried out by scanning electron microscopy and confocal laser scanning microscopy techniques. Gene expressions of osteocalcin, collagen 1, and RUNX2 were carried out using qRT-PCR analyses. Significantly higher (p < 0.05) levels of ALP activity were observed with extended osteoblast induction on the mangiferin-incorporated BCP scaffolds. After characterization of the specimens, it was found that the scaffolds with 10% Mn-incorporated BCP with mangiferin showed better osteogenicity and simultaneously the same scaffolds exhibited higher anti-bacterial properties as observed from the bacterial viability test. This study was carried out to evaluate the efficacy of Mn and MAN in BCP for osteogenicity and antibacterial action.

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“…Lanceros-Méndez et al 35 proposed that this rise may be associated with the interaction between scaffold and water brought on by the surface-bound P OH À groups in composites. Due Furthermore, due to its excellent biocompatibility and bioactivity, 47 HA is taken as a component of biomedical scaffold such that water uptake ability, as well as the roughness of the surface of the PVDF-TrFE polymer, are improved, making them more favorable to adherence of cells. Previously, it was also stated that the charged surface following polarization can considerably boost cell development.…”

Section: Fluorescence Microscopymentioning

confidence: 99%

Synthetic strategy for the production of electrically polarized polyvinylidene fluoride‐trifluoroethylene—co‐polymer osseo‐functionalized with hydroxyapatite scaffold

Swain,

Lenka,

Rautray

2024

J Biomedical Materials Res

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The physiological mechanism of bone tissue regeneration is intricately organized and involves several cell types, intracellular, and extracellular molecular signaling networks. To overcome the drawbacks of autografts and allografts, a number of synthetically produced scaffolds have been manufactured by integrating ceramics, polymers, and their hybrid‐composites. Considering the fact that natural bone is composed primarily of collagen and hydroxyapatite, ceramic‐polymer composite materials seem to be the most viable alternative to bone implants. Here, in this experimental study, copolymer PVDF‐TrFE has been amalgamated with HA ceramics to produce composite scaffolds as bone implants. In order to fabricate PVDF‐TrFE‐HA (polyvinylidene fluoride‐trifluoroethylene—hydroxyapatite) composite scaffolds, solvent casting‐particulate leaching technique was devised. Two scaffold specimens were produced, with different PVDF‐TrFE and HA molar ratios (70:30 and 50:50), and then electrically polarized to observe the subsequent polarization impact on the tissue growth and the suppression of bacterial cell proliferation. Both the specimens underwent characterization to analyze their biocompatibility and bactericidal activities. The bacterial culture of Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) bacteria on the composites was studied to understand the antibacterial characteristics. Moreover, MG63 cells cultured on these as‐formed composites provided information about osteogenesis. Improved osteogenesis and antibacterial efficacy were observed on both the composites. However, the composite with 70 wt% PVDF‐TrFE and 30 wt% HA showed a higher bactericidal effect as well as osteogenesis. It was found that PVDF‐TrFE‐HA‐based biomaterials have the potential for bone tissue engineering applications.

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Biocompatibility and adhesion response of magnesium-hydroxyapatite/strontium-titania (Mg-HAp/ Sr-TiO₂) bilayer coating on titanium

Cited by 23 publications

References 40 publications

Magnesium-Substituted Hydroxyapatite /Spider Silk fibroin /N-Carboxymethyl Chitosan Based Microsphere: A potential template for bone regeneration

Magnesium-Substituted Hydroxyapatite /Spider Silk fibroin /N-Carboxymethyl Chitosan Based Microsphere: A potential template for bone regeneration

Mangiferin-Enriched Mn–Hydroxyapatite Coupled with β-TCP Scaffolds Simultaneously Exhibit Osteogenicity and Anti-Bacterial Efficacy

Synthetic strategy for the production of electrically polarized polyvinylidene fluoride‐trifluoroethylene—co‐polymer osseo‐functionalized with hydroxyapatite scaffold

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Biocompatibility and adhesion response of magnesium-hydroxyapatite/strontium-titania (Mg-HAp/ Sr-TiO2) bilayer coating on titanium

Cited by 23 publications

References 40 publications

Magnesium-Substituted Hydroxyapatite /Spider Silk fibroin /N-Carboxymethyl Chitosan Based Microsphere: A potential template for bone regeneration

Magnesium-Substituted Hydroxyapatite /Spider Silk fibroin /N-Carboxymethyl Chitosan Based Microsphere: A potential template for bone regeneration

Mangiferin-Enriched Mn–Hydroxyapatite Coupled with β-TCP Scaffolds Simultaneously Exhibit Osteogenicity and Anti-Bacterial Efficacy

Synthetic strategy for the production of electrically polarized polyvinylidene fluoride‐trifluoroethylene—co‐polymer osseo‐functionalized with hydroxyapatite scaffold

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Biocompatibility and adhesion response of magnesium-hydroxyapatite/strontium-titania (Mg-HAp/ Sr-TiO₂) bilayer coating on titanium