Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes

Radunović, Milena; Pavić, Aleksandar; Ivanovic, Vera; Milivojević, Marija; Radović, Igor; Carlo, Roberta Di; Pilato, Serena; Fontana, Antonella; Piattelli, Adriano; Petrović, Sanja Matić

doi:10.1016/j.dental.2022.04.024

Cited by 8 publications

(7 citation statements)

References 39 publications

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“…In addition, GO possesses anti-inflammatory and antibacterial properties. As reported by Radunovic et al, many biomaterials, such as titanium disks and collagen membranes functionalized with GO, showed a reduced bacterial biofilm formation when compared with non-functionalized biomaterials [ 15 ]. AFM was used to characterize the surface morphology of the samples.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, it can be easily dispersed in water, and its functional groups allow easy further functionalization or grafting. Studies have proven that graphene and GO are highly biocompatible with low toxicity levels [ 15 ] and excellent cytocompatibility [ 16 ], which enhance their use as a support for tissue regeneration, cell growth, and cell differentiation [ 17 ], at least for the concentration of 10 μg/mL or lower [ 18 ]. The innumerable properties of graphene and its derivatives have prompted biomedical research to evaluate the possible application of these materials in the medical field and to study their interaction with the biological system [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide

Fonticoli

Diomede

Nanci

et al. 2023

IJMS

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Graphene oxide (GO), derived from graphene, has remarkable chemical–physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties. The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E). The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis. Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E. The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR. Together, these results suggest that enriched PPSTBs-GO modulates the inflammatory process through TLR4/MyD 88/NFκB p65/NLRP3 pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide

Fonticoli

Diomede

Nanci

et al. 2023

IJMS

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“…A strong reduction in bacterial growth of the tested bacterial species was observed. 67 Farajzadeh et al synthesized some new metal phthalocyanines bearing fluorinated phenoxy groups and studied their antibiofilm activities against S. aureus and P. aeruginosa. The most effective antibiofilm activity was obtained 93.09% against P. aeruginosa at 500 mg/L.…”

Section: ■ Introductionmentioning

confidence: 99%

Biological Performance of Hexadeca-Substituted Metal Phthalocyanine/Reduced Graphene Oxide Nanobioagents

Farajzadeh Öztürk,

Özdemir,

Yalçın

et al. 2024

ACS Appl. Bio Mater.

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This study presents a tetra-substituted phthalonitrile derivative, namely, diethyl 2-(3,4dicyano-2,5-bis(hexyloxy)-6-(4-(trifluoromethoxy)phenoxy)phenyl)malonate (a), cyclotetramerizing in the presence of some metal salts. The resultant hexadeca-substituted metal phthalocyanines [M= Co, Zn, InCl)] (b−d) were used for the modification of reduced graphene oxide for the first time. The effect of the phthalonitrile/metal phthalocyanines on biological features of reduced graphene oxide (rGO) was extensively examined by the investigation of antioxidant, antimicrobial, DNA cleavage, cell viability, and antibiofilm activities of nanobioagents (1−4). The results were compared with those of unmodified rGO (nanobioagent 5), as well. Modification of reduced graphene oxide with the synthesized compounds improved its antioxidant activity. The antioxidant activities of all the tested nanobioagents also enhanced as the concentration increased. The antibacterial activities of all the nanobioagents improved by applying the photodynamic therapeutic (PDT) method. All the phthalonitrile/phthalocyanine-based nanobioagents (especially phthalocyanine-based nanocomposites) exhibited DNA cleavage activities, and complete DNA fragmentation was observed for nanobioagents (1−4) at 200 mg/L. They can be used as potent antimicrobial and antimicrobial photodynamic therapy agents as well as Escherichia coli microbial cell inhibitors. As a result, the prepared nanocomposites can be considered promising candidates for biomedicine.

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“…Moreover, the natural propensity of graphene-based nanostructures to undergo self-assembly has been widely explored to build up “all-carbon” three-dimensional scaffolds for application in nanomedicine. , In the last decade, different graphene-based biomaterials have been investigated, demonstrating that the properties of the produced scaffold can vary tremendously as a result of the chosen preparation methodology, − surface morphology, type of graphene exploited, its chemical functionalization, defects, and environmental conditions/stimulations . Since many factors and parameters are involved, the engineering or design of the scaffold of interest has to be carefully studied to ensure reproducibility and fine tuning of the properties in view of the desired application. , Among the graphene-based materials, graphene oxide (GO) represents the “hydrophilic derivative” of graphene, and it is usually preferred over graphene for producing homogeneous aqueous suspensions due to the oxygen-containing functional groups on its basal plane (hydroxyl and epoxide groups) and edges (carboxyl groups). , GO exhibits a relatively low electrical conductivity compared to graphene sheets, but it has been shown that GO can support attachment, growth, and differentiation of cells with little or no cytotoxic effects. − Three-dimensional GO foams have been prepared via self-assembly of reduced GO and nanohydroxyapatite composites for tissue engineering applications . Similarly, rolled graphene oxide foams, prepared by sedimentation of GO sheets, have been investigated for the differentiation of stem cells and regeneration of nervous systems …”

Section: Introductionmentioning

confidence: 99%

3D Graphene Oxide-Polyethylenimine Scaffolds for Cardiac Tissue Engineering

Pilato

Moffa

Siani

et al. 2023

ACS Appl. Mater. Interfaces

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The development of novel three-dimensional (3D) nanomaterials combining high biocompatibility, precise mechanical characteristics, electrical conductivity, and controlled pore size to enable cell and nutrient permeation is highly sought after for cardiac tissue engineering applications including repair of damaged heart tissues following myocardial infarction and heart failure. Such unique characteristics can collectively be found in hybrid, highly porous tridimensional scaffolds based on chemically functionalized graphene oxide (GO). By exploiting the rich reactivity of the GO's basal epoxydic and edge carboxylate moieties when interacting, respectively, with NH 2 and NH 3 + groups of linear polyethylenimines (PEIs), 3D architectures with variable thickness and porosity can be manufactured, making use of the layer-by-layer technique through the subsequent dipping in GO and PEI aqueous solutions, thereby attaining enhanced compositional and structural control. The elasticity modulus of the hybrid material is found to depend on scaffold's thickness, with the lowest value of 13 GPa obtained in samples containing the highest number of alternating layers. Thanks to the amino-rich composition of the hybrid and the established biocompatibility of GO, the scaffolds do not exhibit cytotoxicity; they promote cardiac muscle HL-1 cell adhesion and growth without interfering with the cell morphology and increasing cardiac markers such as Connexin-43 and Nkx 2.5. Our novel strategy for scaffold preparation thus overcomes the drawbacks associated with the limited processability of pristine graphene and low GO conductivity, and it enables the production of biocompatible 3D GO scaffolds covalently functionalized with amino-based spacers, which is advantageous for cardiac tissue engineering applications. In particular, they displayed a significant increase in the number of gap junctions compared to HL-1 cultured on CTRL substrates, which render them key components for repairing damaged heart tissues as well as being used for 3D in vitro cardiac modeling investigations.

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Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes

Cited by 8 publications

References 39 publications

Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide

Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide

Biological Performance of Hexadeca-Substituted Metal Phthalocyanine/Reduced Graphene Oxide Nanobioagents

3D Graphene Oxide-Polyethylenimine Scaffolds for Cardiac Tissue Engineering

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