Biocompatibility and drug release behavior of scaffolds prepared by coaxial electrospinning of poly(butylene succinate) and polyethylene glycol

Abstract

Scaffolds constituted by electrospun microfibers of poly(ethylene glycol) (PEG) and poly(butylene succinate) (PBS) were studied. Specifically, coaxial microfibers having different core-shell distributions and compositions were considered as well as uniaxial micro/nanofibers prepared from mixtures of both polymers. Processing conditions were optimized for all geometries and compositions and resulting morphologies (i.e. diameter and surface texture) characterized by scanning electron microscopy. Chemical composition, molecular interactions and thermal properties were evaluated by FTIR, NMR, XPS and differential scanning calorimetry. The PEG component of electrospun fibers could be solubilized by immersion of scaffolds in aqueous medium, giving rise to high porosity and hydrophobic samples. Nevertheless, a small amount of PEG was retained in the PBS matrix, suggesting some degree of mixing. Solubilization was slightly dependent on fiber structure; specifically, the distribution of PEG in the core or shell of coaxial fibers led to higher or lower retention levels, respectively. Scaffolds could be effectively loaded with hydrophobic drugs having antibacterial and anticarcinogenic activities like triclosan and curcumin, respectively. Their release was highly dependent on their chemical structure and medium composition. Thus, low and high release rates were observed in phosphate buffer saline (SS) and SS/ethanol (30:70 v/v), respectively. Slight differences in the release of triclosan were found depending on fiber distribution and composition. Antibacterial activity and biocompatibility were evaluated for both loaded and unloaded scaffolds.

Electrosprayed poly(butylene succinate) microspheres loaded with indole derivatives: A system with anticancer activity

“…It was clearly observed that the unloaded PBS mat with a 3D structure supports a higher cell growth than the control (2D surface of the tissue culture plate). This point is meaningful since it suggests that the mat has a structure of pores and channels similar to other scaffolds developed for tissue growth, and also that PBS has good biocompatibility [61]. On the other hand, mats loaded with indole and indole-derivatives showed inhibitory effects on proliferation of cell lines.…”

Antimicrobial Electrospun Fibers of Polyester Loaded with Engineered Cyclic Gramicidin Analogues

“…High-resolution XPS spectra in the C 1s region are displayed in Figure 5, while those in the N 1s and O 1s regions are displayed in Figure 6. The three Gaussian curves derived from the deconvolution of the C 1s peak for unloaded polyester fibers correspond to the saturated C-C/C-H (284.8 eV), C-O (286.1 eV) and C=O (288.7 eV) bonds of the PE44 chains [50,51]. The three peptide-loaded PE44 fibers display an additional fourth Gaussian curve at 287.7 eV that has been attributed to the (O=)C-NH peptide bonds [52,53].…”

“…The three peptide-loaded PE44 fibers display an additional fourth Gaussian curve at 287.7 eV that has been attributed to the (O=)C-NH peptide bonds [52,53]. On the other hand, two Gaussian curves, which correspond to the C=O (531.87 eV) and C-O bonds (533.42), were derived from the O 1s signal [50][51][52][53][54]. The intensity of the C=O component is lower than that of the C-O one for unloaded PE44, while the opposite is obtained for PE44/GA-S1, PE44/GA-S2, and PE44/GA-L due to the incorporation of peptides.…”

Poly (Butylene Succinate) Scaffolds Prepared by Leaching

“…Literature shows PBS associated with other polymers as an efficient matrix to the preparation of scaffold. [13][14][15][16] Mostly, expensive techniques, such as electro-spinning, are used to the preparation of these materials. 16 Thus, PBS was used here to prepare scaffolds by leaching using NaCl as a cheap porogenic agent.…”

“…[13][14][15][16] Mostly, expensive techniques, such as electro-spinning, are used to the preparation of these materials. 16 Thus, PBS was used here to prepare scaffolds by leaching using NaCl as a cheap porogenic agent. The obtained materials were characterized using several techniques, and the results allowed inferring that leaching is a fast, little expensive and efficient way to prepare PBS scaffolds.…”