Biocompatibility and resorption of a brushite calcium phosphate cement

Theiss, Felix; Apelt, Detlef; Brand, Bastian; Kutter, Annette P N; Zlinszky, K.; Bohner, Marc; Matter, S.; Frei, C.; Auer, Jörg A; Rechenberg, Brigitte von

doi:10.1016/j.biomaterials.2004.11.056

Cited by 276 publications

(260 citation statements)

References 36 publications

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“…Osteoclast size was quantified in SEM micrographs (x 100) by calculating the mean surface area of cells at 3 random locations per disc using automated threshold, edge detection, and particle NL Davison et al Surface architecture of tricalcium phosphate analysis functions in ImageJ software, as previously described (Dalby et al, 2004). Only cells whose area was > 200 μm 2 were included in the analysis to safely exclude mononuclear cells (Theiss et al, 2005). n = 3 replicate discs were analysed per condition.…”

Section: Sem Of Osteoclast Morphologymentioning

confidence: 99%

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Davison

Luo²,

Schoenmaker³

et al. 2014

eCM

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A current challenge of synthetic bone graft substitute design is to induce bone formation at a similar rate to its biological resorption, matching bone's intrinsic osteoinductivity and capacity for remodelling. We hypothesise that both osteoinduction and resorption can be achieved by altering surface microstructure of beta-tricalcium phosphate (TCP). To test this, two TCP ceramics are engineered with equivalent chemistry and macrostructure but with either submicron-or micron-scale surface architecture. In vitro, submicron-scale surface architecture differentiates larger, more active osteoclasts -a cell type shown to be important for both TCP resorption and osteogenesis -and enhances their secretion of osteogenic factors to induce osteoblast differentiation of human mesenchymal stem cells. In an intramuscular model, submicrostructured TCP forms 20 % bone in the free space, is resorbed by 24 %, and is densely populated by multinucleated osteoclast-like cells after 12 weeks; however, TCP with micron-scale surface architecture forms no bone, is essentially not resorbed, and contains scarce osteoclast-like cells. Thus, a novel submicron-structured TCP induces substantial bone formation and is resorbed at an equivalent rate, potentially through the control of osteoclast-like cells.

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Section: Sem Of Osteoclast Morphologymentioning

confidence: 99%

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Davison

Luo²,

Schoenmaker³

et al. 2014

eCM

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“…Brushite is an injectable cement for use in bone replacement. It is commercially available, and is known to be a biocompatible material 7,8) that is easily absorbed and has bone-inducing properties. Carlisle reported silicon is a possible factor in bone calcifi cation 9) in 1970.…”

Section: Application Of Trial Calcium Silicate Powder To Dental Cementmentioning

confidence: 99%

Production of a calcium silicate cement material from alginate impression material

2012

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The purpose of this study was to synthesize biomaterials from daily dental waste. Since alginate impression material contains silica and calcium salts, we aimed to synthesize calcium silicate cement from alginate impression material. Gypsum-based investment material was also investigated as control. X-ray diffraction analyses revealed that although fi ring the set gypsum-based and modifi ed investment materials at 1,200°C produced calcium silicates, fi ring the set alginate impression material did not. However, we succeeded when fi ring the set blend of pre-fi red set alginate impression material and gypsum at 1,200°C. SEM observations of the powder revealed that the featured porous structures of diatomite as an alginate impression material component appeared useful for synthesizing calcium silicates. Experimentally fabricated calcium silicate powder was successfully mixed with phosphoric acid solution and set by depositing the brushite. Therefore, we conclude that the production of calcium silicate cement material is possible from waste alginate impression material.

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“…Cranial sections were obtained frontally, at the level of the maximum diameter of the craniotomy, and femoral sections were obtained sagittally. Sections were radiographed, ground down to 30-40 μm and polished as described elsewhere [33]. They were stained with toluidine blue, which in its orthochromatic phase, stains bone components in various shades of blue, enabling the distinction between the non-mineralized osteoid and the mineralized bone matrix.…”

Section: Histological Evaluationmentioning

confidence: 99%

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Montjovent

Mark

Mathieu

et al. 2008

Bone

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Fetal bone cells were shown to have an interesting potential for therapeutic use in bone tissue engineering due to their rapid growth rate and their ability to differentiate into mature osteoblasts in vitro. We describe hereafter their capability to promote bone repair in vivo when combined with porous scaffolds based on poly(L-lactic acid) (PLA) obtained by supercritical gas foaming and reinforced with 5 wt.% β-tricalcium phosphate (TCP).Bone regeneration was assessed by radiography and histology after implantation of PLA/TCP scaffolds alone, seeded with primary fetal bone cells, or coated with demineralized bone matrix. Craniotomy critical size defects and drill defects in the femoral condyle in rats were employed. In the cranial defects, polymer degradation and cortical bone regeneration were studied up to 12 months postoperatively. Complete bone ingrowth was observed after implantation of PLA/TCP constructs seeded with human fetal bone cells. Further tests were conducted in the trabecular neighborhood of femoral condyles, where scaffolds seeded with fetal bone cells also promoted bone repair.We present here a promising approach for bone tissue engineering using human primary fetal bone cells in combination with porous PLA/TCP structures. Fetal bone cells could be selected regarding osteogenic and immune-related properties, along with their rapid growth, ease of cell banking and associated safety.

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Biocompatibility and resorption of a brushite calcium phosphate cement

Cited by 276 publications

References 36 publications

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Submicron-scale surface architecture of tricalcium phosphate directs osteogenesis in vitro and in vivo

Production of a calcium silicate cement material from alginate impression material

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

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