Biocompatibility enhancement of hemodialysis membranes using a novel zwitterionic copolymer: Experimental, in situ synchrotron imaging, molecular docking, and clinical inflammatory biomarkers investigations

Saadati, Shaghayegh; Westphalen, Heloisa; Eduok, Ubong; Abdelrasoul, Amira; Shoker, Ahmed; Choi, Phillip; Doan, Huu; Ein‐Mozaffari, Farhad; Zhu, Ning

doi:10.1016/j.msec.2020.111301

Cited by 38 publications

(26 citation statements)

References 40 publications

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“…Our recently published paper reveals the utility of docking simulations for assessing the interactions of PES as well as ZW-PES with human serum proteins. 34 Our research group recently reported a series of investigations on the common clinical HD membranes available in Canadian hospitals (PAES and CTA) to explore the key reasons behind their susceptibility to blood activation and unstable cytokines. [35][36][37] We also reported the improved biocompatibility of PES clinical HD membranes modified with novel ZW copolymers.…”

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confidence: 99%

“…Our recently published paper reveals some aspects of FB interaction with the aryl sulfone structure. 34 Herein, we propose the immobilization of HEMA and PMEA structures on PAES and CTA membranes using a real and feasible chemical scenario. An MD simulation framework we recently introduced 29,34 is used here to measure the binding energy between three blood proteins (fibrinogen, FB; human serum albumin, HAS; and human serum transferrin, TRF) and different membrane models to further understand the differences and extent of interaction of each structure with the proteins.…”

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confidence: 99%

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Effects of mussel‐inspired co‐deposition of 2‐hydroxymethyl methacrylate and poly (2‐methoxyethyl acrylate) on the hydrophilicity and binding tendency of common hemodialysis membranes: Molecular dynamics simulations and molecular docking studies

2021

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Despite advances in the field, hemoincompatibility remains a critical issue for hemodialysis (HD) as interactions between various human blood constituents and the polymeric structure of HD membranes results in complications such as activation of immune system cascades. Adding hydrophilic polymer structures to the membranes is one modification approach that can decrease the extent of protein adsorption. This study conducted molecular dynamics (MD) simulations to understand the interactions between three human serum proteins (fibrinogen [FB], human serum albumin, and transferrin) and common HD membranes in untreated and modified forms. Poly(aryl ether sulfone) (PAES) and cellulose triacetate were used as the common dialyzer polymers, and membrane modifications were performed with 2-hydroxymethyl methacrylate (HEMA) and poly (2-methoxyethyl acrylate) (PMEA), using polydopamine-assisted co-deposition. The MD simulations were used as the framework for binding energy simulations, and molecular docking simulations were also performed to conduct molecular-level investigations between the two modifying polymers (HEMA and PMEA) and FB. Each of the three proteins acted differently with the membranes due to their unique nature and surface chemistry. The simulations show PMEA binds less intensively to FB with a higher number of hydrogen bonds, which reflects PMEA's superior performance compared to HEMA. The simulations suggest PAES membranes could be used in modified forms for blood-contact applications as they reflect the lowest binding energy to blood proteins.

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confidence: 99%

Effects of mussel‐inspired co‐deposition of 2‐hydroxymethyl methacrylate and poly (2‐methoxyethyl acrylate) on the hydrophilicity and binding tendency of common hemodialysis membranes: Molecular dynamics simulations and molecular docking studies

2021

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“…On the other hand, the slight decrease in IL-6 may indicate an improvement in anti-inflammatory response, which is in accordance with our previous observations. 21 Serpin is a clotting factor and its release upon interaction with HD membrane has been observed to be strongly dependent of the hydrodynamic conditions applied, but not significantly affected by membrane properties. 36 Therefore, as expected, the concentration of Serpin was not significantly different between coated and bare membranes (p <.346) as seen in Figure S3e.…”

Section: Proposing Anti-fb Adsorption Mechanism For Zwitterionic Coated Membranementioning

confidence: 99%

“…19,20 Our research group has investigated the hemobiocompatibility of clinical polyarylethersulfone (PAES) and cellulose triacetate HD membranes 7,18 as well as PES membranes modified with new sulfonatederived ZW polymers. 21 Enhanced biocompatibility was induced by adding neutral ZW materials on the PES HD membrane, and docking studies suggest the membrane's sulfone functional groups play an essential key role during fibrinogen (FB) interaction and adsorption.…”

Section: Introductionmentioning

confidence: 99%

“…We have also recently investigated the influence of hydrophilicity as an important surface parameter toward biocompatibility enhancement for ZW-coated PAES membranes and PVDF membranes. [19][20][21] The docking studies revealed the sulfone functional groups of the PES membranes or sulfobetaine ZW-coated membranes play an important role in the adsorption of proteins and receptors 7,21 ; while the phosphobetaine polymer has ability to form an electrostatically neutral zwitterionic hydration layer that is capable of acting as a physical barrier against the adsorption of FB. 19,20 With the aim to design HD membrane that improve the biocompatibility profiles of clinical membranes utilized in modern dialyzers, this study focused on the hemocompatibility of PES clinical HD membranes using novel phosphobtaine ZW.…”

Section: Introductionmentioning

confidence: 99%

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Influence of UV‐irradiation intensity and exposure duration on the hemobiocompatibility enhancement of a novel synthesized phosphobetaine zwitterions polyethersulfone clinical hemodialysis membranes

et al. 2021

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To improve the biocompatibility of polyethersulfone (PES) membranes utilized for biomedical hemodialysis (HD) applications, surface grafting with hydrophilic polymers has become a reliable modification strategy. Like most photochemical catalyzed reactions, UV-assisted grafting is distinctly advantageous for inducing permanent surface chemistry, enhancing hydrophilicity, improving morphology, and surface charge of membranes. PES membranes may be hydrophilic and chemically stable; however, they also have low protein-binding capacity and very susceptible to fouling and target analyte binding. In this study, novel zwitterionic polymers (PVP-ZW) have been synthesized by UV-assisted grafting PVP to a phosphobetaine monomer in a reaction involving dimethylamino and dioxaphospholane-2-oxide terminal groups in an NVP monomer solution at varying UV exposure conditions. The highlight of the present study is the investigation of the hemocompatibility of coated PES HD membranes at varying UV exposure conditions with respect to membrane chemistry and morphology and its influence on human serum protein adsorption. A clinical investigation of inflammatory biomarker release from incubated coated membranes within uremic blood samples of HD patients reveals they are weak complement and coagulation activators compared to bare PES membrane. The trend of fibrinogen adsorption on coated PES membranes was observed to increase with reducing UV intensity and exposure duration. Fibrinogen adhesion only increased with roughened membrane surfaces, and this also led to the formation of biological activation pathways hindering biocompatibility. Resistance against fibrinogen absorption on zwitterionic modified PES membrane could be linked with the creation of electrostatically induced neutral zwitterionic PVP-phosphobetaine hydration layer with hydrophilic character.Experimental results are accompanied by spectroscopic and morphological imaging evidence. Zwitterion coated PES membranes (PES-PVP-ZW) fabricated from higher UV intensities through longer exposure durations showed significant presence of surface deformations in the forms of inherent exfoliations due to harsh UV reaction conditions. The zeta potential and surface roughness of coated membranes also played significant role in the fibrinogen adsorption on PES membranes during ultrafiltration.

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Robust fabrication of poly(lactic acid) membrane with good hemocompatibility over heparin‐mimetic graphene‐based nanosheets

Huang

Chen

et al. 2022

J of Applied Polymer Sci

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Developing dislysis membranes with outstanding hemocompatibility is the key of hemodialysis used for curing the kidney malfunction. Herein, polylactic acid (PLA) membrane with high water flux (640.2 L m À2 h À1 ) and bovine serum albumin (BSA) rejection (>97%) is prepared and subsequently modified by heparin-mimetic sulfonated graphene oxide (SGO) via modified musselinspired method. The (NH 4 ) 2 Fe(SO 4 ) 2 serves as Fenton catalyst significantly accelerates the oxidized polymerization of dopamine (DA) in DA-g-SGO to form polydopamine-g-SGO (PDA-g-SGO). The uniform immobilization of SGO significantly improves the hydrophilicity and electronegativity of the PLA membranes, inhibiting platelets adhesion. Moreover, the sulfonic group of SGO plays the role of anticoagulant as heparin. The obtained PLA/PDA-g-SGO membrane exhibits excellent hemocompatibility with longer recalcification time (230 s) and lower hemolysis ratio (1.6%) than those of original PLA membrane (120 s, 10.7%). This study demonstrates a potential way to improve the hemocompatibility of polymeric membranes.

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Biocompatibility enhancement of hemodialysis membranes using a novel zwitterionic copolymer: Experimental, in situ synchrotron imaging, molecular docking, and clinical inflammatory biomarkers investigations

Cited by 38 publications

References 40 publications

Effects of mussel‐inspired co‐deposition of 2‐hydroxymethyl methacrylate and poly (2‐methoxyethyl acrylate) on the hydrophilicity and binding tendency of common hemodialysis membranes: Molecular dynamics simulations and molecular docking studies

Effects of mussel‐inspired co‐deposition of 2‐hydroxymethyl methacrylate and poly (2‐methoxyethyl acrylate) on the hydrophilicity and binding tendency of common hemodialysis membranes: Molecular dynamics simulations and molecular docking studies

Influence of UV‐irradiation intensity and exposure duration on the hemobiocompatibility enhancement of a novel synthesized phosphobetaine zwitterions polyethersulfone clinical hemodialysis membranes

Robust fabrication of poly(lactic acid) membrane with good hemocompatibility over heparin‐mimetic graphene‐based nanosheets

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