Biocompatibility evaluation of a novel hydroxyapatite-polymer coating for medical implants (in vitro tests)

Negroiu, Gabriela; Piticescu, Roxana Mioara; Chiţanu, Gabrielle Charlotte; Mihăilescu, I. N.; Zdrentu, L.; Miroiu, Marimona

doi:10.1007/s10856-007-3300-6

Cited by 47 publications

(15 citation statements)

References 26 publications

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“…The examples include deposition calcium orthophosphate-based biocomposites with sodium maleate (Negroiu et al 2008), alendronate (Bigi et al 2009) and silk fibroin (Miroiu et al 2010). Thermally unstable calcium orthophosphates, such as OCP (Boanini et al 2012), might be deposited as well.…”

Section: Reviewmentioning

confidence: 99%

Calcium orthophosphate coatings, films and layers

Dorozhkin¹

2012

Prog Biomater

119

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In surgical disciplines, where bones have to be repaired, augmented or improved, bone substitutes are essential. Therefore, an interest has dramatically increased in application of synthetic bone grafts. As various interactions among cells, surrounding tissues and implanted biomaterials always occur at the interfaces, the surface properties of the implants are of the paramount importance in determining both the biological response to implants and the material response to the physiological conditions. Hence, a surface engineering is aimed to modify both the biomaterials, themselves, and biological responses through introducing desirable changes to the surface properties of the implants but still maintaining their bulk mechanical properties. To fulfill these requirements, a special class of artificial bone grafts has been introduced in 1976. It is composed of various mechanically stable (therefore, suitable for load bearing applications) biomaterials and/or bio-devices with calcium orthophosphate coatings, films and layers on their surfaces to both improve interactions with the surrounding tissues and provide an adequate bonding to bones. Many production techniques of calcium orthophosphate coatings, films and layers have been already invented and new promising techniques are continuously investigated. These specialized coatings, films and layers used to improve the surface properties of various types of artificial implants are the topic of this review.Electronic supplementary materialThe online version of this article (doi:10.1186/2194-0517-1-1) contains supplementary material, which is available to authorized users.

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Section: Reviewmentioning

confidence: 99%

Calcium orthophosphate coatings, films and layers

Dorozhkin¹

2012

Prog Biomater

119

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“…Primary hMSC were obtained from bone marrow aspirates and isolated by density gradient centrifugation, as previously described in Ref. [31]. Bone marrow was harvested from one healthy patient undergoing surgery for the orthopaedic implant procedure, with the approval of the Ethics Committee of the University of Medicine and Pharmacy of Craiova (Reference No.…”

Section: Cell Culturementioning

confidence: 99%

Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts

et al. 2019

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This study is focused on the adhesion and differentiation of the human primary mesenchymal stem cells (hMSC) to osteoblasts lineage on biological-derived hydroxyapatite (BHA) and lithium-doped BHA (BHA:LiP) coatings synthesized by Pulsed Laser Deposition. An optimum adhesion of the cells on the surface of BHA:LiP coatings compared to control (uncoated Ti) was demonstrated using immunofluorescence labelling of actin and vinculin, two proteins involved in the initiation of the cell adhesion process. BHA:LiP coatings were also found to favor the differentiation of the hMSC towards an osteoblastic phenotype in the presence of osteoinductive medium, as revealed by the evaluation of osteoblast-specific markers, osteocalcin and alkaline phosphatase. Numerous nodules of mineralization secreted from osteoblast cells grown on the surface of BHA:LiP coatings and a 3D network-like organization of cells interconnected into the extracellular matrix were evidenced. These findings highlight the good biocompatibility of the BHA coatings and demonstrate that the use of lithium as a doping agent results in an enhanced osteointegration potential of the synthesized biomaterials, which might therefore represent viable candidates for future in vivo applications.

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“…In vitro , hydroxyapatite materials have been demonstrated to be compatible with mesenchymal stem cells, stimulating their adhesion and differentiation into osteoblastic cell lines, primary human osteoblast cells, human embryonic kidney cell lines and human keratinocyte cell lines (Douglas et al , 2009;Guo et al , 2009;Negroiu et al , 2008;Sandeman et al , 2009;Shadanbaz and Dias, 2012;Zhang et al , 2009b). Superior osteoconductivity of hydroxyapatite was confi rmed in vivo using several animal © Woodhead Publishing Limited, 2014 8.5 Different morphologies of hydroxyapatite nano-and microcrystals obtained via a facile hydrothermal process at different pH values: (a,b) nanorods, pH = 7.0; (c,d) burr-like microspheres, pH = 5.0;…”

Section: Hydroxyapatitementioning

confidence: 99%